GEO DataSets

Analysis of Pseudomonas aeruginosa cultures exposed to the macrolide azithromycin (AZM). P. aeruginosa infection is the major cause of morbidity and mortality in cystic fibrosis patients. Results provide insight into mechanisms underlying the beneficial role of AZM therapy in infection control.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL84

Series:

GSE2430

4 Samples

Download data: CEL, EXP

(Submitter supplied) Experimental Design Type of experiment: In order to evaluate the influence of subinhibitory concentrations of the macrolide antibiotic Azithromycin (AZM) on the global Pseudomonas aeruginosa gene expression, we performed a comparative gene expression analysis of AZM-treated versus untreated P. aeruginosa PAO1 cultures. Experimental factors: P. aeruginosa PAO1 cultures with and without addition of 2 µg/ ml AZM were grown until early stationary phase. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Dataset:

GDS2111

Platform:

GPL84

4 Samples

Download data: CEL, EXP

(Submitter supplied) P. aeruginosa produces serious chronic infections in hospitalized patients and immunocompromised individuals, including cystic fibrosis patients. The molecular mechanisms by which P. aeruginosa responds to antibiotics and other stresses to promote persistent infections may provide new avenues for therapeutic intervention. Azithromycin (AZM), an antibiotic frequently utilized in cystic fibrosis treatment, is thought to improve clinical outcomes through a number of mechanisms including impaired biofilm growth and quorum sensing in P. more...

Organism:

Pseudomonas aeruginosa PA14

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31013

12 Samples

Download data: BED, BEDGRAPH, GFF3, TAB

(Submitter supplied) Antibiotics in low concentrations may have an array of effects. We have exposed growing P. aeruginosa cells to different classes of antibiotics in order to investigate this hypothesis. We have used DNA microarrays to investigate the effect of low concentrations of antibiotics (azithromycin, ceftazidime and ciprofloxacin) on the gene expression of P. aeruginosa aiming to elucidate general trends. Keywords: Treatment with antibiotics

Organism:

Pseudomonas aeruginosa PAO1; Pseudomonas aeruginosa

Type:

Expression profiling by array

Platform:

GPL84

12 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) Purpose : The goal of this study was to use RNA Seq to define the regulon of the transciption factor Anr by comparing global transcriptional profiles of Pseudomonas aeruginosa strain PAO1 and a clinical isolate with their isogenic ∆anr mutants, grown in colony biofilms at 1% oxygen. Methods : mRNA profiles were generated for laboratory strain PAO1 and for a clinical isolate J215, as well as for ∆anr derivatives of each strain, in duplicate, by deep sequencing. more...

Organism:

Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18782 GPL18287

8 Samples

Download data: XLSX

(Submitter supplied) Low-dose macrolides are effective therapy in patients with chronic lung infections, but the mechanisms of action are unclear. We compared global gene expression profiles between P.aeruginosa with and without low-dose Azithromycin (AZM) to study why the low-dose macrolide therapy is effective for cystic fibrosis and diffuse panbronchiolitis. Keywords: dose-response

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Dataset:

GDS3562

Platform:

GPL84

5 Samples

Download data: CEL

Analysis of PAO1 cultures exposed to up to 2.0 ug/ml of the macrolide azithromycin (AZM) for 9 hours. Low-dose macrolides are effective therapy in patients with chronic lung infections such as those caused by P. aeruginosa. Results provide insight into mechanism of action of AZM against infection.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array, count, 2 agent, 3 dose sets

Platform:

GPL84

Series:

GSE12738

5 Samples

Download data: CEL

(Submitter supplied) Microarrays were used to evaluate the effects of azithromycin and an inflammatory stimulus (SMM) on human airway epithelium. Effects of azithromycin treatment were evaluated at 6, 24 and 48 hours. Effects of SMM were evaluated at 6 and 24 hours. In addition, pretreatment with azithromycin was used to evaluate the modulatory effects on SMM-induced inflammation. SMM=supernatant from microcorpulent material from human cystic fibrosis airways. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

34 Samples

Download data: CEL

(Submitter supplied) GAPDHs from human pathogens S. aureus and P. aeruginosa can be readily inhibited by ROS-mediated direct oxidation of their catalytic active cysteines. Because of the rapid degradation of H2O2 by bacterial catalase, only steady-state but not one-dose treatment of H2O2 induces rapid metabolic reroute from glycolysis to pentose phosphate pathway (PPP). We conducted RNA-seq analyses to globally profile the bacterial transcriptomes in response to a steady level of H2O2, which reveals profound transcriptional changes including the induced expression of glycolytic genes in both bacteria. more...

Organism:

Staphylococcus aureus subsp. aureus str. Newman; Pseudomonas aeruginosa MPAO1/P1

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18362 GPL18363

8 Samples

Download data: DIFF