GEO DataSets

(Submitter supplied) Ectomycorrhizal (ECM) fungi are crucial for tree nitrogen (N) nutrition, however, mechanisms governing N transfer from fungal tissues to the host plant are not well understood. ECM fungal isolates, even from the same species, vary considerably in their ability to support tree N nutrition resulting in a range of often unpredictable symbiotic outcomes. In this study, we used isotopic labelling to quantify the transfer of N to the plant host by isolates from the ECM genus Pisolithus known to have significant variability in colonisation and transfer of nutrients to a host. more...

Organism:

Pisolithus microcarpus; Eucalyptus grandis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL33522 GPL33523 GPL33524

43 Samples

Download data: TXT

(Submitter supplied) Illumina HiSeq technology was used to generate mRNA profiles from Pisolithus microcarpus Si9, MW3, R4 or R10 ectomycorrhizal root tips compared to their respective free-living mycelium.

Organism:

Eucalyptus grandis; Pisolithus microcarpus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL19282 GPL28947

23 Samples

Download data: TXT

(Submitter supplied) Illumina HiSeq technology was used to generate mRNA profiles from in vitro Eucalyptus grandis roots interacting with two different Pisolithus microcarpus strains (SI-9 and SI-12) and under two different CO2 concentrations (400 and 650 ppm) . Control roots or ectomycorrhizal root tips were harvested after 1 month and used for RNA extraction. Paired-end (2X150bp) reads were generated and aligned to Eucalyptus grandis transcripts (http://www.phytozome.net/; primarytranscripts only) using CLC Genomics Workbench 6.

Organism:

Eucalyptus grandis; Pisolithus microcarpus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL19282 GPL19281

23 Samples

Download data: TXT

(Submitter supplied) Illumina HiSeq technology was used to generate mRNA profiles from Serapias vomeracea-Tulasnella calospora mycorrhizal protocorms compared to asymbiotic protocorms . Protocorms were harvested at stage P2 and used for RNA extraction. Reads of 150bp were generated and aligned to a de novo transcriptome assembly from Serapias vomeracea using CLC Genomics Workbench 9.

Organism:

Tulasnella calospora; Serapias vomeracea

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL22451 GPL22437

6 Samples

Download data: FASTA, TXT

(Submitter supplied) Illumina HiSeq technology was used to generate mRNA profiles from Tulasnella calospora mycorrhizal protocorms at stage P2 compared to free-living mycelium grown on Modified Melin-Norkrans medium with two different N sources, ammonium (AL13M) and glutamine (AL1 G) . Protocorms were harvested at stage P2 and mycelium after 15 days and used for RNA extraction. Reads of 150bp were generated and aligned to Tulasnella calospora transcripts (http://genome.jgi-psf.org/Tulca1) using CLC Genomics Workbench 9.

Organism:

Tulasnella calospora; Serapias vomeracea

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL22436 GPL22437

9 Samples

Download data: TXT

(Submitter supplied) Many trees form ectomycorrhizal symbiosis with fungi. During symbiosis, the tree roots supply sugar to the fungi in exchange for nitrogen, and this process is critical for the nitrogen and carbon cycles in forest ecosystems. However, the extents to which ectomycorrhizal fungi can liberate nitrogen and modify the soil organic matter and the mechanisms by which they do so remain unclear since they have lost many enzymes for litter decomposition that were present in their free-living, saprotrophic ancestors. more...

Organism:

Paxillus involutus; Laccaria bicolor

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL19637 GPL19511

24 Samples

Download data: TAB

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Paxillus involutus ATCC 200175; Paxillus involutus

Type:

Expression profiling by array

Platform:

GPL14950

21 Samples

Download data: PAIR

(Submitter supplied) Abstract: Proteins contribute to a major part of the organic nitrogen in forest soils. This nitrogen is mobilized and becomes available to trees as a result of the depolymerizing activities of symbiotic ectomycorrhizal fungi. The mechanisms by which these fungi depolymerize proteins and assimilate the released nitrogen are poorly characterized. Biochemical analysis and transcriptome profiling were performed to examine the proteolytic machinery and the uptake system of the ectomycorrhizal basidiomycete Paxillus involutus during the assimilation of organic nitrogen from various protein sources and extracts of organic matter. more...

Organism:

Paxillus involutus ATCC 200175; Paxillus involutus

Type:

Expression profiling by array

Platform:

GPL14950

12 Samples

Download data: PAIR

(Submitter supplied) Abstract: Proteins contribute to a major part of the organic nitrogen in forest soils. This nitrogen is mobilized and becomes available to trees as a result of the depolymerizing activities of symbiotic ectomycorrhizal fungi. The mechanisms by which these fungi depolymerize proteins and assimilate the released nitrogen are poorly characterized. Biochemical analysis and transcriptome profiling were performed to examine the proteolytic machinery and the uptake system of the ectomycorrhizal basidiomycete Paxillus involutus during the assimilation of organic nitrogen from various protein sources and extracts of organic matter. more...

Organism:

Paxillus involutus ATCC 200175; Paxillus involutus

Type:

Expression profiling by array

Platform:

GPL14950

9 Samples

Download data: PAIR