GEO DataSets

(Submitter supplied) The discovery of FoxO1 as an effector of insulin action on gene expression filled a gap in our knowledge of insulin signaling. The metabolic impact of hepatic FoxO1 has been demonstrated in genetic mouse models showing that FoxO1 inhibition can reverse diabetes. However, the gamut of FoxO1 targets is unknown, due to the lack of robust genome-wide chromatin occupancy data. To elucidate the genomic architecture of the FoxO1 effect on liver metabolism, we integrated genome-wide ChIP-seq and RNA-seq. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

24 Samples

Download data: DIFF, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

32 Samples

Download data: TDF, TXT

(Submitter supplied) The discovery of FoxO1 as an effector of insulin action on gene expression filled a gap in our knowledge of insulin signaling. The metabolic impact of hepatic FoxO1 has been demonstrated in genetic mouse models showing that FoxO1 inhibition can reverse diabetes. However, the gamut of FoxO1 targets is unknown, due to the lack of robust genome-wide chromatin occupancy data. To elucidate the genomic architecture of the FoxO1 effect on liver metabolism, we integrated genome-wide ChIP-seq and RNA-seq. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

8 Samples

Download data: TDF

(Submitter supplied) Insulin integrates hepatic glucose and lipid metabolism, directing nutrients to storage as glycogen and triglyceride. In type 2 diabetes, levels of the former are low and the latter are exaggerated, posing a pathophysiologic and therapeutic conundrum. A branching model1 of insulin signaling, with FoxO1 presiding over glucose production2-5 and Srebp–1c regulating lipogenesis,6-8 provides a potential explanation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6096

12 Samples

Download data: CEL, CHP

(Submitter supplied) Anti-FoxO1 chromatin immunoprecipitation-high throughput sequencing (ChIP-Seq) was performed on mouse heart tissue following 7 days vehicle treatment/sham-operation, isoproterenol injection (subcutaneous, 3mg/kg/day), or transverse aortic constriction (TAC). Pool of 3 hearts per condition.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

4 Samples

Download data: BW, XLSX

(Submitter supplied) ChIP-seq analysis demonstrates FoxK1 binding to proximal promoters and enhancers, especially to genes containing a TGTTTAC motif, which is similar to the FoxA/FoxO motifs.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24676

12 Samples

Download data: BIGWIG, NARROWPEAK

(Submitter supplied) We performed global run-on sequencing in the livers of fasted and refed mice in order to build a comprehensive map of the transcriptional changes in the liver in response to feeding. We further looked at the livers of mice lacking Akt (AktDKO) or Akt and FoxO1 (AktFoxo1TKO) to assess the role of insulin signaling in mediating this feeding response. With this method, we identified 599 feeding-regulated transcripts out of a total of 7,505. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

Download data: BW