GEO DataSets

(Submitter supplied) Quantitative disease resistance (QDR) is a form of plant immunity conserved across species able to limit infections caused by a broad range of pathogens. QDR has a complex genetic determinism, the bases of which are not fully understood. The number of genes contributing to the QDR response and the extent to which molecular components of the QDR response vary across plant species remain elusive. The fungal pathogen Sclerotinia sclerotiorum, causal agent of white mold disease on hundreds of plant species, triggers QDR responses in host populations. more...

Organism:

Beta vulgaris subsp. vulgaris; Phaseolus vulgaris; Ricinus communis; Helianthus annuus

Type:

Expression profiling by high throughput sequencing

4 related Platforms

24 Samples

Download data: TXT

(Submitter supplied) Cooperation is associated with major transitions in evolution such as the emergence of multicellularity. It is central to the evolution of many complex traits in nature, including growth and virulence in pathogenic bacteria. Whether cells of multicellular parasites function cooperatively during infection remains however largely unknown. Here, we show that hyphal cells of the fungal pathogen Sclerotinia sclerotiorum reprogram towards division of labor to facilitate the colonization of host plants. more...

Organism:

Sclerotinia sclerotiorum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20037

15 Samples

Download data: TXT

(Submitter supplied) Plant pathogens with a broad host range are able to infect plant lineages that diverged over 100 million years ago. They exert similar and recurring constraints on the evolution of unrelated plant populations. Plants generally respond with quantitative disease resistance (QDR), a form of immunity relying on complex genetic determinants. In most cases, the molecular determinants of QDR and how they evolve is unknown. more...

Organism:

Arabidopsis thaliana; Solanum lycopersicum

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL17639 GPL19694

12 Samples

Download data: TXT

(Submitter supplied) RNA-seq of Sclerotinia sclerotiorum-infected B. napus leaves during a timecourse after inoculation.

Organism:

Sclerotinia sclerotiorum; Brassica napus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL22102 GPL22103

21 Samples

Download data: XLSX

(Submitter supplied) Sclerotinia sclerotiorum is a broad-host range necrotrophic pathogen which is the causative agent of Sclerotinia stem rot (SSR), and a major disease of soybean (Glycine max). A time course transcriptomic analysis was performed in both compatible and incompatible soybean lines to identify pathogenicity and developmental factors utilized by S. sclerotiorum to achieve pathogenic success.

Organism:

Sclerotinia sclerotiorum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20037

21 Samples

Download data: CSV

(Submitter supplied) The goal of the microarray experiment was to identify defense genes that were differentially expressed in the Arabidopsis mutants med16, med8, elp2, wild type in response to infection of the necrotrophic fungal pathogen Sclerotinia sclerotiorum. Results indicated that, compared with the wild type, the jasmonic acid (JA) biosynthesis genes LOX3, AOC3, and OPR3, ethylene (ET) biosynthesis genes ACS2 and ACS8, as well as salicylic acid (SA) biosynthesis genes ICS1 and EDS5 were up-regulated in med16 in local tissues at 1 day post-inoculation (dpi) and/or systemic tissues at 4 dpi. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL12621

48 Samples

Download data: TXT

(Submitter supplied) In this study, we developed transgenic Arabidopsis thaliana (AT1703) expressing double-stranded (ds)RNA to silence S. sclerotiorum ABHYDROLASE-3 and slow infection through host induced gene silencing (HIGS). Leaf infection assays show reduced S. sclerotiorum lesion size, fungal load, and ABHYDROLASE-3 transcript abundance in AT1703 lines compared to wild-type Col-0. To better understand how HIGS influences host-pathogen interactions, we performed global RNA sequencing on AT1703 and wild-type Col-0 lines directly at the site of S. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21785

17 Samples

Download data: TXT

(Submitter supplied) The Solexa/Illumina’s digital gene expression (DGE) system was used to gain insight into the broad range of transcriptional responses during the vegetative growth, sclerotial development, myceliogenic germination, carpogenic germination, apothecium formation (stipe) and infection of Sclerotinia sclerotiorum. We obtained a sequencing depth of approximately 3.3 million clean tags per cDNA library. Tag mapping indicated that these six cDNA libraries in total represented more than 66.7% of all of the genes presented in the predicted transcript databases of the Broad Institute. more...

Organism:

Sclerotinia sclerotiorum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13429

6 Samples

Download data: TXT

(Submitter supplied) The host range of parasites is an important factor in assessing the dynamics of disease epidemics. The evolution of pathogens to accommodate new hosts may lead to host range expansion, a process the molecular bases of which are largely enigmatic. The fungus Sclerotinia sclerotiorum parasitizes more than 400 plant species from diverse eudicot families while its close relative, S. trifoliorum, is restricted to plants from the Fabaceae family. more...

Organism:

Sclerotinia trifoliorum; Sclerotinia sclerotiorum 1980 UF-70

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29264 GPL29266

57 Samples

Download data: TXT

(Submitter supplied) The free-living soil fungus Trichoderma hamatum GD12 is notable amongst other Trichoderma strains in exhibiting both biocontrol and plant growth promotion (PGP) activities, which are coincident with a markedly expanded genome when compared to other characterised biocontrol and PGP isolates. Here, we make direct comparisons of T. hamatum GD12 transcription during PGP, and during antagonism of the root-infecting pathogen Sclerotinia sclerotiorum, in peat-based microcosms. more...

Organism:

Sclerotinia sclerotiorum; Trichoderma hamatum GD12

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20037 GPL20047 GPL20048

52 Samples

Download data: TXT

(Submitter supplied) Sclerotinia sclerotiorum is a pathogenic fungus that infects hundreds of crop species, causing extensive yield loss every year. Chemical fungicides are used to control this phytopathogen, but with concerns about increasing resistance and impacts on non-target species, there is a need to develop alternative control measures. In the present study, we engineered Brassica napus to constitutively express a hairpin (hp)RNA molecule to silence ABHYRDOLASE-3 in S. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26890

18 Samples

Download data: XLSX

(Submitter supplied) Background: The biological control agent Pseudomonas chlororaphis PA23 is effective at protecting Brassica napus (canola) from the necrotrophic fungus Sclerotinia sclerotiorum via direct antagonism. Despite the growing importance of biocontrol bacteria in plant protection from fungal pathogens, little is known about how the host plant responds to bacterial priming on the leaf surface or about changes in gene activity genome-wide in the presence and absence of S. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15579

12 Samples

Download data: DIFF, FPKM_TRACKING, TXT

(Submitter supplied) Colletotrichum gloeosporioides, a widely distributed economically important agent in postharvest fruit disease thrives by massive secretion of ammonia to alkalize its environment and activate its pathogenicity genes. We sequenced the C. gloeosporioides transcriptomes of WT and a ∆pacC mutant, a major gene regulator under changing pH, under pH 7.0 in order to examine pacC regulation. Transcriptome analysis of the ∆pacC strain showed that 468 and 458 genes were up or down regulated, respectively. more...

Organism:

Colletotrichum gloeosporioides

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16217

2 Samples

Download data: FASTA, GTF, XLSX

(Submitter supplied) This study provides a first large-scale cloning and characterization of Sclerotinia sclerotiorum milRNAs and milRNAs candidates. Two microRNA-like RNAs (milRNAs) and 42 milRNA candidates were identified by sequence analysis. These milRNAs and candidates provide new insights into the functional roles of small RNAs and adds new resources for the study of plant pathogenic fungi.

Organism:

Sclerotinia sclerotiorum

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL13429

1 Sample

Download data: TXT

(Submitter supplied) Alcohol oxidases are ecologically important enzymes which facilitate a number of plant-fungal interactions. Within Ascomycota they are primarily associated with methylotrophy, as a peroxisomal alcohol oxidase (AOX) catalyzing the conversion of methanol to formaldehyde in methylotrophic yeast. In this study we demonstrate that AOX orthologs are phylogenetically conserved proteins which are common in the genomes of non-methylotrophic, plant-associating fungi. more...

Organism:

Sclerotinia sclerotiorum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32607

6 Samples

Download data: XLS

(Submitter supplied) Secreted laccases are important enzymes on an ecological scale for their role in mediating plant-fungal interactions, but their function in fungal pathogenesis has yet to be elucidated. Ascomycete laccases have been primarily associagted with cell wall melanin deposition, and laccase mutants in ascomycete species often demonstrate reduced pigmentation. In this study, a putatively secreted laccase, Sslac2, was characterized from the broad host-range plant pathogen Sclerotinia sclerotiorum, which is largely unpigmented and is not dependent on melanogenesis for plant infection. more...

Organism:

Sclerotinia sclerotiorum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32607

12 Samples

Download data: TXT

(Submitter supplied) We sequenced the complete genome of an ectophytic fungus, Ramichloridium luteum, which colonizes the surface of apple fruit, and carried out comparative genomic and transcriptome analysis

Organism:

Ramichloridium luteum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23608

6 Samples

Download data: TXT