GEO DataSets

(Submitter supplied) Background: Cardiac kinases play a critical role in the development of heart failure, and represent potential tractable therapeutic targets. However, only a very small fraction of the cardiac kinome has been investigated. To identify novel cardiac kinases involved in heart failure, we employed an integrated transcriptomics and bioinformatics analysis and identified Homeodomain-Interacting Protein Kinase 2 (HIPK2) as a novel candidate kinase. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

9 Samples

Download data: CEL

(Submitter supplied) We use Affymetrix Gene Chip Array to screen the targets of HIPK2 in heart. Total RNA was extracted from 8-week-old HIPK2-knockout and Wild Type (WT) mice.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL21163

10 Samples

Download data: TXT

(Submitter supplied) Cardiac hypertrophy can lead to heart failure, and is induced either by physiological stimuli eg postnatal development, chronic exrcise training or pathological stimuli eg pressure or volume overload. This data set looks at microRNA profiles in mouse models to examine whether phosphoinositide 3-kinase (p110 alpha isoform) activity is critical for the maintenance of cardiac function and long term survival in a seeting of heart failure (myocardial infarction). more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL8824

24 Samples

Download data: TXT

(Submitter supplied) Cardiac hypertrophy can lead to heart failure, and is induced either by physiological stimuli eg postnatal development, chronic exercise training or pathological stimuli eg pressure or volume overload. Majority of new therapies for heart failure has mixed outcomes. A combined mouse model and oligo-array approach are used to examine whether phosphoinositide 3-kinase (p110-alpha isoform) activity is critical for maintenance of cardiac function and long-term survival in a setting of heart failure. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

24 Samples

Download data: CEL, CHP

(Submitter supplied) Analysis of the role of Hipk2 in regulating gene expression in medullary thymic epithelial cells. The whole genome gene signatures of purified mTEC subsets (CD80 low, CD80 high) from TEC-specific Hipk2 knockout mice were compared to mating wildtype control mice (floxed, Cre-). Results provide the up- or down-regulated genes, affected by the Hipk2 gene knockout.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL17543

8 Samples

Download data: IDAT, TXT

(Submitter supplied) We used an integrated computational/experimental systems biology approach to identify upstream protein kinases that regulate gene expression changes in kidneys of HIV-1 transgenic mice (Tg26), which have significant tubulo-interstitial fibrosis (TIF) and glomerulosclerosis (GS). We identified the homeo-domain interacting protein kinase 2 (HIPK2) as a key regulator of TIF and GS. HIPK2 was upregulated in kidneys of Tg26 and patients with various kidney diseases. more...

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS4233 GDS4234

Platforms:

GPL1261 GPL6244

24 Samples

Download data: CEL, CHP

Analysis of kidney cortices from HIV-1 transgenic, FVB/N males and females (Tg26). Tg26 models the renal pathologic changes observed in human disease and is used to study HIV-associated nephropathy (HIVAN) pathogenesis. Results provide insight into the molecular mechanisms underlying HIVAN.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE35226

12 Samples

Download data: CEL, CHP

Analysis of HEK293 cells transfected with homeo-domain interacting protein kinase 2 (HIPK2) and HIPK2-DN mutant. HIPK2 mediates renal fibrosis in 3 mouse models of renal fibrosis (Tg26, UUO, folic-acidinduced renal fibrosis). Results provide further insight into role of HIPK2 in kidney fibrosis.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 genotype/variation sets

Platform:

GPL6244

Series:

GSE35226

12 Samples

Download data: CEL, CHP

(Submitter supplied) Non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) is a conserved protein found in higher plants. In photosynthetic cells, the enzyme is involved in a shuttle transfer mechanism to export NADPH from the chloroplast to the cytosol. In this work, we demonstrate that GAPN gene express in leaves and roots at similar levels; showing the highest level of expression in flowers. To investigate the role of this enzyme in different plant tissues, we characterized a mutant from Arabidopsis thaliana having an insertion at the GAPN gene locus. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL1941

3 Samples

Download data

(Submitter supplied) Three major MAP kinase signaling cascades, ERK, p38 and JNK, play significant roles in the development of cardiac hypertrophy and heart failure in response to external stress and neural/hormonal stimuli. In order to study the specific function of each MAP kinase branch in adult heart, we have generated three transgenic mouse models with cardiac specific and temporally regulated expression of activated mutants of Ras, MKK3 and MKK7, which are selective upstream activators for ERK, p38 and JNK, respectively. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1306

Platform:

GPL1261

36 Samples

Download data

Analysis of adult hearts after induction of activated transgene mutants of Ras, MKK3, or MKK7, which are specific upstream activators of the MAP kinases ERK, p38, and JNK respectively. Hearts examined at various time points up to 4 weeks following each transgene induction using tamoxifen.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 gender, 4 genotype/variation, 3 time sets

Platform:

GPL1261

Series:

GSE3530

36 Samples

Download data

(Submitter supplied) The fatty acid synthase (FASN) is the major fat synthesizing enzyme. FASN is an indispensable enzyme because mice with genetic deletion of Fasn are not viable. Apart from its physiological function, previous studies indicated that FASN could also exert a pathophysiological role, in the heart, because patients with heart failure showed up-reguation of FASN. To investigate the in vivo function of FASN up-regulation in the heart, we generated mice with myocardium-specific expression of FASN under control of the alpha-MHC promoter. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

(Submitter supplied) The anti-diabetic drug and agonist of the peroxisome proliferator-activated receptor gamma (Pparg), rosiglitazone, was recently withdrawn in many countries because the drug use was associated with an increased risk of heart failure. To investigate underlying pathomechanisms, we chose 6-month-old apolipoprotein E (apoE)-deficient mice, which are prone to atherosclerosis and insulin resistance, and thereby mimic the risk profile of patients with cardiovascular disease. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

(Submitter supplied) Primary murine fetal liver cells were freshly isolated from day e14.5 livers and then sorted for successive differentiation stages by Ter119 and CD71 surface expression (ranging from double-negative CFU-Es to Ter-119 positive enucleated erythrocytes) [Zhang, et al. Blood. 2003 Dec 1; 102(12):3938-46]. RNA isolated from each freshly isolated, stage-sorted population was reverse-transcribed, labelled, and then hybridized onto 3' oligo Affymetrix arrays. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

11 Samples

Download data: CEL, TXT

(Submitter supplied) Expression profiling of fetal liver erythroid precursors after either Hipk1 or Hipk2 knockdown by shRNA versus control shRNA

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL8860

4 Samples

Download data: TXT

(Submitter supplied) Background The contribution of glucocorticoids to sexual dimorphism in the heart is essentially unknown. Therefore, we sought to determine the sexually dimorphic actions of glucocorticoid signaling in cardiac function and gene expression. To accomplish this goal, we conducted studies on mice lacking glucocorticoid receptors (GR) in cardiomyocytes (cardioGRKO mouse model). Methods and Results Deletion of cardiomyocyte GR leads to an increase in mortality because of the development of spontaneous cardiac pathology in both male and female mice; however, females are more resistant to GR signaling inactivation in the heart. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

12 Samples

Download data: TXT

(Submitter supplied) Candidate cardiomyocyte (CM) mitogens such as those affecting ERK signaling pathway represent potential targets for functional heart regeneration. We explored whether activating ERK via a constitutively active mutant of BRAF, BRAF-V600E (caBRAF), can induce pro-proliferative effects in neonatal rat engineered cardiac tissues (ECTs). Sustained CM-specific caBRAF expression induced chronic ERK activation, significant tissue growth, deficit in sarcomeres and contractile function, and tissue stiffening, all of which persisted for at least 4 weeks of culture. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL14844

12 Samples

Download data: XLSX

(Submitter supplied) Recently, it is reported that multiple signaling pathways are involved with the pathogenesis of PPCM. Here, we show that 23k PRL activates PERK signaling.

Organism:

Rattus norvegicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18694

9 Samples

Download data: BW

(Submitter supplied) Recently, it is reported that multiple signaling pathways are involved with the pathogenesis of PPCM. Here, we show that PERK kockout affect these pathways in postpartum period.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

12 Samples

Download data: BIGWIG

(Submitter supplied) Numerous murine models of heart failure (HF) have been described, many of which develop progressive deterioration of cardiac function. We have recently demonstrated that several of these can be "rescued" or prevented by transgenic cardiac expression of a peptide inhibitor of the beta-adrenergic receptor kinase (betaARKct). To uncover genomic changes associated with cardiomyopathy and/or its phenotypic rescue by the betaARKct, oligonucleotide microarray analysis of left ventricular (LV) gene expression was performed in a total of 53 samples, including 12 each of Normal, HF, and Rescue. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS411 GDS427

Platforms:

GPL75 GPL76

106 Samples

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