Similar studies for GEO DataSets (Select 200130974) - GEO DataSets

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Items: 20

Select item 2001309741.

Transcriptome analysis of ITGA3+ and ITGA3- from expanded CB cells

(Submitter supplied) Cell purification technology combined with whole transcriptome sequencing and small molecule agonist of hematopoietic stem cell self-renewal has allowed us to identify ITGA3 as a surface maker that defines a rare subpopulation of human cells which is highly enriched for stem cell activity in vivo. ITGA3-positive cells (within the EPCR+CD90+CD133+CD34+CD45RA- fraction) exhibit a robust multi-lineage differentiation potential and serial reconstitution in immunocompromised mice. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18573
7 Samples

Download data: TSV

Series

Accession:: GSE130974

ID:: 200130974

Select item 2000771282.

EPCR Expression Defines the Most Primitive Subset of Human HSPC and Is Required for Their In Vivo Activity

(Submitter supplied) Cell purification technology combined with whole transcriptome sequencing and small molecule agonist of hematopoietic stem cell self-renewal has allowed us to identify the endothelial protein c receptor protein (EPCR) as a surface maker that defines a rare subpopulation of human cells which is highly enriched for stem cell activity in vivo. EPCR-positive cells exhibit a robust multi-lineage differentiation potential and serial reconstitution in immunocompromised mice. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL11154
12 Samples

Download data: TXT

Series

Accession:: GSE77128

ID:: 200077128

Select item 2001519643.

Transcriptome data of uncultured and HGFs along with 0.1% DMSO or LY plus Rapa or SR1-treated hUCB CD34+ cells

(Submitter supplied) mRNA profiling of uncultured and HGFs along with 0.1% DMSO (HGFs) or LY plus Rapa (LR) or SR1-treated hUCB CD34+ cells

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL20795
4 Samples

Download data: TXT

Series

Accession:: GSE151964

ID:: 200151964

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 2001003544.

Highly purified human cord blood-derived CD34+/- hematopoietic stem cells

(Submitter supplied) Global gene expressions of human cord blood-derived 18Lineage-negative (18Lin-)CD34+CD38-CD133+GPI-80+ cells (CD34+ HSCs), 18Lin-CD34-CD133+GPI-80+ cells (CD34- HSCs) and 18Lin-CD34+CD133- cells (non-HSCs) were analyzed. Results provide an insight into the molecular mechanisms underlying the self-renewal, maintenance and differentiation of human cord blood-derived CD34+/- HSCs.

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL20844
9 Samples

Download data: TXT

Series

Accession:: GSE100354

ID:: 200100354

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Select item 2001327475.

Gene Expression data human HSCs with 2-LSD1 inhibitors

(Submitter supplied) Human CB HSC enriched fractions were treated with LSD1 inhibitor 2-PCPA and UM171 to identify differntially regulated genes by these compounds.

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL16686
16 Samples

Download data: CEL

Series

Accession:: GSE132747

ID:: 200132747

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Select item 2001551806.

Mitochondrial activity linked to CD34+CD90+EPCR+ phenotype determines the functional fitness of ex vivo expanded HSCs

(Submitter supplied) We report the transcritpome of purified CD34+ cells from cultures initiated with cord blood CD34+ hematopoietic stem cells that were expanded ex vivo in Stemline II media supplemented with a cytokine cocktail in the presence or absence of valproic acid for 6 days .

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL16791
6 Samples

Download data: TXT

Series

Accession:: GSE155180

ID:: 200155180

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Select item 2001925197.

Single cell transcriptome sequencing of expanded human hematopoieic stem/progenitor cells cultured in PCL-PVAc-PEG based 3a medium and StemSpan with UM171/SR-1 medium.

(Submitter supplied) We demonstrated that chemically difined PCL-PVAc-PEG based 3a medium was superior to conventional StemSpan besed medium in expansion of human hematopoietic stem cells. In fact, more phenotypic HSCs (CD34+EPCR+CD90+CD45RA-ITGA3+) were expanded in our culture condition and higher donor chmerism was achieved in transplantation assay using humanized mice. But detail transcriptome profiles of expanded HSCs was unknown and therefore single-cell RNA sequencing was done to reveal it. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL20795
3 Samples

Download data: MTX, RDS, TSV

Series

Accession:: GSE192519

ID:: 200192519

PubMed Similar studies

Select item 2001913388.

Transcriptomes of cultured human hematopoietic stem cells.

(Submitter supplied) We compared transcriptomes of CD34(high)EPCR-positive with CD34(high)EPCR-negative cells after 10-days culture in PCL-PVAc-PEG based 3a medium. It was because our culture condition had not previously reported and so we demonstrated that phenotypic HSCs were concentrated in EPCR-positive fraction as conventional UM171-based culture system. As a result, CD34(high)EPCR-positive cells highly expressed HSC markers (HLF, AVP, PRDM16 and FGD5) compared with CD34(high)EPCR-negative cells.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18573
6 Samples

Download data: TXT

Series

Accession:: GSE191338

ID:: 200191338

Select item 2000349749.

Gene expression of cultured human fetal liver hematopoietic stem and progenitor cells (HSPC) and their supportive ex vivo OP9 stromal niche cells

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens; Mus musculus

Type:: Expression profiling by array

Platforms:: GPL1261 GPL570
17 Samples

Download data: CEL

Series

Accession:: GSE34974

ID:: 200034974

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Select item 20003497310.

Gene expression data from the mesenchymal stem cell line OP9M2 that supported expansion of human hematopoietic stem and progenitor cells in vitro

(Submitter supplied) One of the long-standing goals in the field has been to establish a culture system that would allow maintenance of HSC properties ex vivo. In the absence of such system, the ability to model human hematopoiesis in vitro has been limited, and there has been little progress in the expansion of human HSCs for clinical application. To that end, we defined a mesenchymal stem cell co-culture system based on a monoclonal OP9 stromal cell line (OP9M2), for expansion of clonally multipotent human HSPCs that were protected from apoptosis and immediate differentiation, and retained the HSPC phenotype. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
4 Samples

Download data: CEL

Series

Accession:: GSE34973

ID:: 200034973

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Select item 20003497211.

Gene expression analysis of human fetal liver hematopoietic stem and progenitor cells (HSPC) in culture

(Submitter supplied) One of the long-standing goals in the field has been to establish a culture system that would allow maintenance of HSC properties ex vivo. In the absence of such system, the ability to model human hematopoiesis in vitro has been limited, and there has been little progress in the expansion of human HSCs for clinical application. To that end, we defined a mesenchyml stem cell co-culture system for expansion of clonally multipotent human HSPCs that are protected from apoptosis and immediate differentiation, and retain the HSPC phenotype. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL570
13 Samples

Download data: CEL

Series

Accession:: GSE34972

ID:: 200034972

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Select item 20007068512.

Musashi-2 attenuates AHR signalling to expand human haematopoietic stem cells

(Submitter supplied) A greater understanding of the molecular pathways that underpin the unique human hematopoietic stem and progenitor cell (HSPC) self-renewal program will improve strategies to expand these critical cell types for regenerative therapies. The post-transcriptional mechanisms guiding HSPC fate during ex vivo expansion have not been closely investigated. Using shRNA-mediated knockdown, we show that the RNA-binding protein (RBP) Musashi-2 (MSI2) is required for human HSPC self-renewal. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL11154
8 Samples

Download data: XLSX

Series

Accession:: GSE70685

ID:: 200070685

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Select item 20006958313.

Musashi-2 Post-transcriptionally Attenuates Aryl Hydrocarbon Receptor Signaling to Expand Human Hematopoietic Stem Cells

Organism:: Homo sapiens

Type:: Other

Platform:: GPL11154
2 Samples

Download data: BED, BW

Series

Accession:: GSE69583

ID:: 200069583

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Select item 20002910514.

Isolation of single human hematopoietic stem cells capable of long-term multilineage engraftment

(Submitter supplied) Gene expression profiling was used to investigate the relationship between human cord blood hematopoietic stem cells (HSC) and multipotent progenitors described in the study. Genes more highly expressed in HSC may be associated with stem cell function and self-renewal.

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL6947
9 Samples

Download data: TXT

Series

Accession:: GSE29105

ID:: 200029105

Select item 20014081315.

The BET inhibitor CPI203 promotes ex-vivo expansion of long-term repopulating human hematopoietic stem cells and megakaryocyte

(Submitter supplied) RNA sequencing reveals the transcriptomic differences between cord blood cells expanded by CPI203/cytokines against DMSO/cytokines.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24676
12 Samples

Download data: TXT

Series

Accession:: GSE140813

ID:: 200140813

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Select item 20015173316.

Gene expression analysis of hematopoietic stem cells based on Syndecan-2 surface expression

(Submitter supplied) Analysis of how Syndecan-2 expression identifies hematopoietic stem cells with unique gene expression profiles. We tested the hypothesis that Syndecan-2 expression enriches for hematopoietic stem cell gene expression programs. The results provide important insight into how Syndecan-2 can be used to isolate hematopoietic stem cells with distinct hematopoietic properties.

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL21493
9 Samples

Download data: XLSX

Series

Accession:: GSE151733

ID:: 200151733

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20025485717.

MYCT1 Controls Environmental Sensing in human Hematopoietic Stem Cells [scRNA-seq Dextran]

(Submitter supplied) We identified a novel HSC regulatory gene, MYCT1 (MYC target 1), that is selectively expressed in undifferentiated human HSPC and is critical for human HSC expansion and engraftment. MYCT1 knockdown (KD) in human fetal liver and cord blood (CB) HSPCs impaired expansion ex vivo and engraftment after transplantation, whereas restoring the compromised MYCT1 expression via lentiviral overexpression (OE) in cultured human CB HSPCs improved ex vivo expansion of the most undifferentiated human HSPCs (CD34+CD38-CD90+CD45RA-EPCR+ITGA3+) and enhanced their engraftment ability. more...