Similar studies for GEO DataSets (Select 200122339) - GEO DataSets

Select item 2001223391.

An unstructured MET-2/SETDB1 cofactor ensures H3K9me2, focus formation and perinuclear anchoring [RNA-seq]

(Submitter supplied) The segregation of the genome into accessible euchromatin and histone H3 lysine 9 methylated (H3K9me) heterochromatin is essential for the repression of repetitive elements and tissue-specific genes. In C. elegans, the SETDB1 homolog MET-2 catalyzes H3K9me1 and me2. In worms as in vertebrates, the regulation of this crucial enzyme remains enigmatic. Contrary to the localization of overexpressed MET-2, we find endogenous MET-2 to be nuclear throughout development, enriched in perinuclear foci in a cell cycle-dependent manner. more...

Organism:: Caenorhabditis elegans

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18245
12 Samples

Download data: TAB

Series

Accession:: GSE122339

ID:: 200122339

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Select item 2001689252.

A structural role for histone methyltransferase MET-2 represses transcription independent of H3K9me catalysis

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Caenorhabditis elegans

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL18245
47 Samples

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Series

Accession:: GSE168925

ID:: 200168925

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Select item 2001689243.

A structural role for histone methyltransferase MET-2 represses transcription independent of H3K9me catalysis [RNA-seq]

(Submitter supplied) Packaging genomic regions into silenced heterochromatin is critical to maintain organismal viability and tissue identity. Methylation of histone H3 on lysine 9 (H3K9me) marks heterochromatin. Here we show that in addition to catalyzing H3K9me, the C. elegans histone methyltransferase MET-2 (SETDB1-like) has a second non-catalytic function that can itself contribute to gene repression. We find that subnuclear concentrates, or foci, of MET-2 correlate with efficient HMT activity, yet foci composed of catalytic-deficient MET-2 are also able to mediate transcriptional silencing. more...

Organism:: Caenorhabditis elegans

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18245
15 Samples

Download data: TAB

Series

Accession:: GSE168924

ID:: 200168924

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Select item 2001689234.

A structural role for histone methyltransferase MET-2 represses transcription independent of H3K9me catalysis [ChIP-seq]

(Submitter supplied) Packaging genomic regions into silenced heterochromatin is critical to maintain organismal viability and tissue identity. Methylation of histone H3 on lysine 9 (H3K9me) marks heterochromatin. Here we show that in addition to catalyzing H3K9me, the C. elegans histone methyltransferase MET-2 (SETDB1-like) has a second non-catalytic function that can itself contribute to gene repression. We find that subnuclear concentrates, or foci, of MET-2 correlate with efficient HMT activity, yet foci composed of catalytic-deficient MET-2 are also able to mediate transcriptional silencing. more...

Organism:: Caenorhabditis elegans

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL18245
32 Samples

Download data: TAB

Series

Accession:: GSE168923

ID:: 200168923

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Select item 2001223415.

An unstructured MET-2/SETDB1 cofactor ensures H3K9me2, focus formation and perinuclear anchoring

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Caenorhabditis elegans

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL18245
30 Samples

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Series

Accession:: GSE122341

ID:: 200122341

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Select item 2001223356.

An unstructured MET-2/SETDB1 cofactor ensures H3K9me2, focus formation and perinuclear anchoring [ChIP-seq]

(Submitter supplied) The segregation of the genome into accessible euchromatin and histone H3 lysine 9 methylated (H3K9me) heterochromatin is essential for the repression of repetitive elements and tissue-specific genes. In C. elegans, the SETDB1 homolog MET-2 catalyzes H3K9me1 and me2. In worms as in vertebrates, the regulation of this crucial enzyme remains enigmatic. Contrary to the localization of overexpressed MET-2, we find endogenous MET-2 to be nuclear throughout development, enriched in perinuclear foci in a cell cycle-dependent manner. more...

Organism:: Caenorhabditis elegans

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL18245
18 Samples

Download data: TAB

Series

Accession:: GSE122335

ID:: 200122335

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Select item 2001565517.

Two parallel pathways recruit the H3K9me3 HMT in somatic cells, requiring the Argonaut NRDE-3, or the MBT-domain protein, LIN-61

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Caenorhabditis elegans

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:: GPL18245
99 Samples

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Series

Accession:: GSE156551

ID:: 200156551

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Select item 2001565508.

Two parallel pathways recruit the H3K9me3 HMT in somatic cells, requiring the Argonaut NRDE-3, or the MBT-domain protein, LIN-61 (smallRNA-seq)

(Submitter supplied) The establishment and maintenance of chromatin domains shape the epigenetic memory of a cell, with histone H3 lysine 9 methylation defining repressed heterochromatin. We show that in C. elegans the SET-25 (SUV39/G9a) HMT that catalyzes H3K9me1-3, is able to establish repressed domains de novo. We identify here two distinct pathways that recruit SET-25 to its targets. One requires LIN-61 (L3MBTL2), a conserved protein with 4 MBT domains that recognizes H3K9me2 deposited by the HMT MET-2 (SETDB1). more...

Organism:: Caenorhabditis elegans

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL18245
18 Samples

Download data: TAB

Series

Accession:: GSE156550

ID:: 200156550

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Select item 2001565499.

Two parallel pathways recruit the H3K9me3 HMT in somatic cells, requiring the Argonaut NRDE-3, or the MBT-domain protein, LIN-61 (RNA-seq)

(Submitter supplied) The establishment and maintenance of chromatin domains shape the epigenetic memory of a cell, with histone H3 lysine 9 methylation defining repressed heterochromatin. We show that in C. elegans the SET-25 (SUV39/G9a) HMT that catalyzes H3K9me1-3, is able to establish repressed domains de novo. We identify here two distinct pathways that recruit SET-25 to its targets. One requires LIN-61 (L3MBTL2), a conserved protein with 4 MBT domains that recognizes H3K9me2 deposited by the HMT MET-2 (SETDB1). more...

Organism:: Caenorhabditis elegans

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18245
21 Samples

Download data: TAB

Series

Accession:: GSE156549

ID:: 200156549

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Select item 20015654810.

Two parallel pathways recruit the H3K9me3 HMT in somatic cells, requiring the Argonaut NRDE-3, or the MBT-domain protein, LIN-61 (ChIP-seq)

(Submitter supplied) The establishment and maintenance of chromatin domains shape the epigenetic memory of a cell, with histone H3 lysine 9 methylation defining repressed heterochromatin. We show that in C. elegans the SET-25 (SUV39/G9a) HMT that catalyzes H3K9me1-3, is able to establish repressed domains de novo. We identify here two distinct pathways that recruit SET-25 to its targets. One requires LIN-61 (L3MBTL2), a conserved protein with 4 MBT domains that recognizes H3K9me2 deposited by the HMT MET-2 (SETDB1). more...

Organism:: Caenorhabditis elegans

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL18245
60 Samples

Download data: TAB

Series

Accession:: GSE156548

ID:: 200156548

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Select item 20014425311.

Histone H3K9 methylation promotes formation of genome compartments in C. elegans via chromosome compaction and perinuclear anchoring

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Caenorhabditis elegans

Type:: Genome binding/occupancy profiling by high throughput sequencing; Other

Platform:: GPL22765
8 Samples

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Series

Accession:: GSE144253

ID:: 200144253

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Select item 20014425212.

Histone H3K9 methylation promotes formation of genome compartments in C. elegans via chromosome compaction and perinuclear anchoring (Hi-C)

(Submitter supplied) Genomic regions preferentially associate with regions of similar transcriptional activity, partitioning genomes into active and inactive compartments within the nucleus. Here we explored mechanisms controlling genome compartment organization in C. elegans and investigated roles for compartments in regulating gene expression. The distal arms of C. elegans chromosomes, which are enriched for heterochromatic histone modifications including H3K9me, interact with each other both in cis and in trans, while interacting less frequently with central regions of chromosomes, leading to genome compartmentalization. more...

Organism:: Caenorhabditis elegans

Type:: Other

Platform:: GPL22765
2 Samples

Download data: TXT

Series

Accession:: GSE144252

ID:: 200144252

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Select item 20014425113.

Histone H3K9 methylation promotes formation of genome compartments in C. elegans via chromosome compaction and perinuclear anchoring (ChIP-seq)

(Submitter supplied) Genomic regions preferentially associate with regions of similar transcriptional activity, partitioning genomes into active and inactive compartments within the nucleus. Here we explored mechanisms controlling genome compartment organization in C. elegans and investigated roles for compartments in regulating gene expression. The distal arms of C. elegans chromosomes, which are enriched for heterochromatic histone modifications including H3K9me, interact with each other both in cis and in trans, while interacting less frequently with central regions of chromosomes, leading to genome compartmentalization. more...

Organism:: Caenorhabditis elegans

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL22765
6 Samples

Download data: BW

Series

Accession:: GSE144251

ID:: 200144251

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Select item 20011384114.

Genomic distribution of H3K9me2 in wild-type vs. arle-14 mutant C. elegans embryos

(Submitter supplied) We discovered arle-14/B0336.5 as a critical gene for H3K9 di-methylation. To test the hypothesis that ARLE-14 targets the H3K9 methyltransferase MET-2 to specific loci, we compared the distribution of H3K9me2 in wild-type and arle-14 mutants by ChIP-Seq. Our results reveal that H3K9me2 genomic distribution is similar in wild-type vs. mutants and ARLE-14 is not required for targeting MET-2.

Organism:: Caenorhabditis elegans

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL18245
8 Samples

Download data: BW

Series

Accession:: GSE113841

ID:: 200113841

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Select item 20003722615.

LMN-1-DamID in N2 and set-25(n5021) met-2(n4256) mutant early C. elegans embryos

(Submitter supplied) We asked if the perinuclear position of chromosome arms in C. elegans depends on the histone methyltransferases MET-2 and SET-25. To this end, we performed LMN-1-DamID in wild-type (N2) and mutant (set-25 met-2) strains. LMN-1-DamID signal on chromosome arms was significantly reduced in the mutant.

Organism:: Caenorhabditis elegans

Type:: Other

Platform:: GPL8134
6 Samples

Download data: PAIR, TAB

Series

Accession:: GSE37226

ID:: 200037226

Select item 20016716816.

H3K9me blocks transcription factor activity in differentiated cells to ensure tissue integrity

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Caenorhabditis elegans

Type:: Genome binding/occupancy profiling by high throughput sequencing; Other; Expression profiling by high throughput sequencing

Platform:: GPL18245
40 Samples

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Series

Accession:: GSE167168

ID:: 200167168

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Select item 20016716717.

H3K9me blocks transcription factor activity in differentiated cells to ensure tissue integrity [RNA-seq]

(Submitter supplied) Development in multicellular organisms is governed by a carefully orchestrated program of gene expression controlled by both genetic and epigenetic factors1,2. Histone H3 lysine 9 methylation (H3K9me) is the defining modification of heterochromatin, which is thought to have two main functions. It silences satellite repeats and transposable elements to ensure genome stability3, and stabilizes differentiated states by repressing tissue-specific genes4,5. more...

Organism:: Caenorhabditis elegans

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18245
22 Samples

Download data: TXT

Series

Accession:: GSE167167

ID:: 200167167

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Select item 20016716618.

H3K9me blocks transcription factor activity in differentiated cells to ensure tissue integrity [CUT&RUN; ChICseq]

(Submitter supplied) Development in multicellular organisms is governed by a carefully orchestrated program of gene expression controlled by both genetic and epigenetic factors1,2. Histone H3 lysine 9 methylation (H3K9me) is the defining modification of heterochromatin, which is thought to have two main functions. It silences satellite repeats and transposable elements to ensure genome stability3, and stabilizes differentiated states by repressing tissue-specific genes4,5. more...

Organism:: Caenorhabditis elegans

Type:: Other

Platform:: GPL18245
6 Samples

Download data: TXT

Series

Accession:: GSE167166

ID:: 200167166

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20016716519.

H3K9me blocks transcription factor activity in differentiated cells to ensure tissue integrity [ATAC-seq]

(Submitter supplied) Development in multicellular organisms is governed by a carefully orchestrated program of gene expression controlled by both genetic and epigenetic factors1,2. Histone H3 lysine 9 methylation (H3K9me) is the defining modification of heterochromatin, which is thought to have two main functions. It silences satellite repeats and transposable elements to ensure genome stability3, and stabilizes differentiated states by repressing tissue-specific genes4,5. more...