GEO DataSets

(Submitter supplied) The glucose/mannose-PTS permease EIIMan encoded by manLMN in the dental caries pathogen Streptococcus mutans has a dominant influence on sugar-specific, CcpA-independent catabolite repression (CR). Mutations in manL affect energy metabolism and virulence-associated traits, including biofilm formation, acid tolerance and competence. Using promoter:reporter fusions, expression of the manLMN and the fruRKI operons, encoding a transcriptional regulator, a fructose-1-P kinase and a fructose-PTS permease EIIFru, respectively, was monitored in response to carbohydrate source and in mutants lacking CcpA, FruR, and components of EIIMan. more...

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21130

9 Samples

Download data: XLSX

(Submitter supplied) The phosphoenolpyruvate:sugar phosphotransferase system (PTS) is the major carbohydrate transport system in oral streptococci. The mannose-PTS of Streptococcus mutans, which transports mannose and glucose, is involved in carbon catabolite repression (CCR) and regulates the expression of known virulence genes. In this study, we investigated the role of EIIGlc and EIIABMan in sugar metabolism, gene regulation, biofilm formation, and competence. more...

Organism:

Streptococcus mutans

Type:

Expression profiling by array

Platform:

GPL4340

12 Samples

Download data: MEV

(Submitter supplied) In this study we analyzed the transcriptional regulation of the operon (OBio) encoding the PTSBio and showed that it was repressed by NigR, a LacI-like transcriptional regulator. To elucidate the role of NigR in regulation of S. mutans carbohydrate transporters we performed full genome expression microarrays using custom Affymetrix chip. Expression microarray analysis was performed with ΔnigR grown in CDM supplemented with glucose and compared to that of WT grown under identical conditions. more...

Organism:

Streptococcus mutans UA159; Streptococcus mutans

Type:

Expression profiling by array

Platform:

GPL4769

4 Samples

Download data: CEL, CHP

(Submitter supplied) Streptococcus mutans, the organism most frequently associated with the development of dental caries, is able to utilize a diverse array of carbohydrates for energy metabolism. One such molecule is trehalose, a disaccharide common in human foods, which has recently been implicated in enhancing the virulence of epidemic strains of the pathogen, Clostridium difficile. In this study, deletion mutants of all three genes in the putative S. more...

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24591

18 Samples

Download data: TXT, XLSX

(Submitter supplied) The Gram-negative bacterium Vibrio cholerae adapts to changes in environment by selectively producing the necessary machinery to uptake and metabolize available carbohydrates. The import of the six-carbon sugar fructose by the fructose-specific phosphoenolpyruvate (PEP) phosphotransferase system (PTS) is of particular interest because of its putative connection to biofilms, which have been shown to promote host colonization and cholera pathogenesis. more...

Organism:

Vibrio cholerae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27062

8 Samples

Download data: TXT

(Submitter supplied) For Streptococcus mutans, the major etiologic agent of human dental caries, sucrose is the carbohydrate that contributes in the most significant manner to establishment, persistence and virulence of the organism. However, because this organism produces multiple extracellular sucrolytic enzymes that can release hexoses from sucrose, it has not been possible to study the specific effects of sucrose transport and metabolism on gene expression in the absence of carbohydrates that by themselves can elicit catabolite repression and induce expression of multiple genes. more...

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21130

12 Samples

Download data: XLSX

(Submitter supplied) Streptococcus mutans, the primary etiologic agent of human dental caries, and a variety of oral Streptococcus and Actinomyces spp. synthesize high molecular mass homopolymers of fructose (fructans) with predominantly β2,1- (inulins) or β2,6-linkages (levans). The ability of S. mutans to degrade fructans contributes to the severity of dental caries. The extracellular product of fruA of S. mutans is an exo-β-D-fructofuranosidase that releases fructose from levan and inulin. more...

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21130

6 Samples

Download data: CSV

(Submitter supplied) The transport of carbohydrates by Streptococcus mutans is accomplished by the phosphoenolpyruvate-phosphotrasferase system (PTS) and ATP-binding cassette (ABC) transporters. To undertake a global transcriptional analysis of all S. mutans sugar transporters simultaneously, we used a whole-genome expression microarray. Global transcription profiles of S. mutans UA159 were determined for several carbohydrates. more...

Organism:

Streptococcus mutans; Streptococcus mutans UA159

Type:

Expression profiling by array

Platform:

GPL4769

48 Samples

Download data: CEL, EXP

(Submitter supplied) The genetic and phenotypic responses of Streptococcus mutans, an organism known to be strongly associated with the development of dental caries, to changes in carbohydrate availability were investigated. S. mutans UA159 or a derivative of UA159 lacking ManL, which is the EIIAB component (EIIABMan) of a glucose/mannose permease of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) and a dominant effector of catabolite repression, were grown in continuous culture to steady-state in conditions of excess (100 mM) or limiting (10 mM) glucose. more...

Organism:

Streptococcus mutans UA159; Streptococcus mutans

Type:

Expression profiling by array

Platform:

GPL15711

6 Samples

Download data: MEV

(Submitter supplied) In this study we showed that one PTS of S. mutans is specific for biofilm mode of growth in the presence of sucrose. Our data indicates that this PTS is involved in transport and metabolism of disaccharides and possibly oligosaccharides synthesized by GtfI and GtfSI.

Organism:

Streptococcus mutans UA159; Streptococcus mutans

Type:

Expression profiling by array

Platform:

GPL4769

22 Samples

Download data: CEL

(Submitter supplied) Multiple levels of interkingdom signaling have been implicated in maintaining the ecological balance between Candida albicans and commensal streptococci to assure a state of oral health. To better understand the molecular mechanisms involved in the initial streptococcal response to the presence of C. albicans that can initiate oral surface colonization and biofilm formation, hypha-forming cells were incubated with Streptococcus gordonii cells for 30 minutes to assess the streptococcal transcriptome response. more...

Organism:

Streptococcus gordonii

Type:

Expression profiling by array

Platform:

GPL20180

2 Samples

Download data: TXT

(Submitter supplied) Iron and manganese are micronutrientients that serve as important co-factors necessary for enzymatic function. In the host environment, access to metals can be highy restricted. Here, we wanted to identify the transcriptomic response when S. mutans UA159 is grown in media depleted of iron, manganese, or both.

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21130

12 Samples

Download data: CSV

(Submitter supplied) Identification of the major PEP-phosphotransferase systems (PTS) for glucose, mannose and cellobiose of Listeria monocytogenes, and their significance for extra- and intracellular growth. Transcriptional profile of prfA, the dependent genes and the PTS genes after growth in MM (minimal medium (Premaratne et al. 2001)) (supplemented with 10 mM Glucose). Listeria monocytogenes EGD-e possesses 86 pts genes encoding 29 complete and several incomplete PEP-dependent phosphotransferase systems (PTS) for the transport of carbohydrates and sugar alcohols. more...

Organism:

Listeria monocytogenes; Listeria monocytogenes EGD-e

Type:

Expression profiling by array

Platform:

GPL6554

24 Samples

Download data: TXT

(Submitter supplied) CcpA is a global regulator of transcription in Gram-positive bacteria that controls gene expression in response to carbohydrate availability. Using the fructan hydrolase (fruA) gene of S. mutans as a model, we demonstrate that CcpA does indeed play a major role in carbohydrate catabolite repression. Using DNA microarrays, the expression of at least 170 genes differed in CcpA-deficient cells compared to the parental strains when cells are grown in the presence of glucose, but only 90 genes showed altered expression when cells were cultivated in the poorly-repressing substrate galactose. more...

Organism:

Streptococcus mutans

Type:

Expression profiling by array

Platform:

GPL4340

16 Samples

Download data: MEV

(Submitter supplied) H2O2 is an oxidative stress agent known to trigger an upregulation in expression of detoxification genes in Streptococcus mutans. Following discovery of a spontaneously-occurring perR mutation, we wanted to compare the transcriptomic responses of H2O2 exposure in S. mutans strains bearing an intact perR or a perR deletion.

Organism:

Streptococcus mutans UA159

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29159

12 Samples

Download data: CSV, XLSX

(Submitter supplied) In this study, we show that ManR (VC1825) activates the transcription of the mannose operon in M9 medium supplemented with fructose or mannose, and when manR is impaired Vibrio cholerae cells fail to colonize the intestine and host survival is significantly prolonged in a fruit fly Drosophila melanogaster infection model. To identify other target genes of ManR, transcriptome analysis was performed in wild-type V. more...

Organism:

Vibrio cholerae O1 biovar El Tor str. N16961

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31160

8 Samples

Download data: XLSX

(Submitter supplied) Streptococcus mutans is a common constituent of oral biofilms and a primary etiologic agent of human dental caries. The bacteria associated with dental caries have a potent ability to produce organic acids from dietary carbohydrates and to grow and metabolize in acidic conditions. In this study, we observed supplementation with 1.5% arginine (final concentration) had inhibitory effects on the growth of S. more...

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21130

18 Samples

Download data: XLSX

(Submitter supplied) Transcriptional profiling of S. mutans rpoE knockout mutant comparing to the wild type. The mutation of rpoE cause dramatic changes in phenotypes. The goal is to determine the genes affected by rpoE. The delta subunit of RNA polymerase, RpoE, is wide spread in low G+C Gram-positive bacteria and is thought to play a role in enhancing transcriptional specificity by blocking RNA polymerase binding at weak-promoter sites and stimulating RNA synthesis by accelerating core enzyme recycling. more...

Organism:

Streptococcus mutans UA159

Type:

Expression profiling by array

Platform:

GPL10540

15 Samples

Download data: TXT

(Submitter supplied) We assesed the transcriptional response of wild-type and isogenic lrgAB mutant to growth in the presence of three different stress conditions (aerobiosis, high-temperature, and the cell-wall active antibiotic vancomycin) by using RNA-seq

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21130

24 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of Haloferax mediterranei DF50 and ΔdeoR2 with induction by fructose comparing with the strains without this induction. Goal was to explore the effect of induction by fructose on Haloferax mediterranei.

Organism:

Haloferax mediterranei

Type:

Expression profiling by array

Platform:

GPL16102

6 Samples

Download data: TXT