GEO DataSets

(Submitter supplied) The phenolic compound salicylic acid (SA) is a key signaling molecule regulating local and systemic plant defense responses against biotrophs. Many microbial organisms, including pathogens, share the ability to degrade SA. However, the mechanism by which they perceive SA is unknown. We showed that Ustilago maydis, the causal agent of corn smut disease, employs a so far uncharacterized SA sensing mechanism. more...

Organism:

Zea mays; Mycosarcoma maydis

Type:

Expression profiling by array

Platform:

GPL22065

6 Samples

Download data: TXT

(Submitter supplied) Ustilago maydis is a plant-pathogenic fungus that establishes a biotrophic relationship with its host Zea mays. The biotrophic interaction is initiated upon host penetration, and involves expansion of the host plasma membrane around hyphae, which is thought to facilitate the exchange of nutrients and virulence factors. Transcriptional regulators involved in the establishment of an infectious dikaryon and penetration into the host have been identified, however, regulators involved in the post-penetration stages remained to be elucidated. more...

Organism:

Zea mays

Type:

Expression profiling by array

Platform:

GPL4032

9 Samples

Download data: CEL

(Submitter supplied) Hxt1 is a high affinity hexose transporter important for virulence of the phytopathogenic basidiomycete Ustilago maydis on its host plant maize. Hxt1 shows the highest similarities to the glucose sensors Rgt2 and Snf3 from S. cereviciae (42% and 39% identity on amino acid level, respectively). In these sensors, the substitution of a highly conserved arginine to lysine leads to a constitutive active signal, resulting in expression of several glucose induced genes. more...

Organism:

Mycosarcoma maydis

Type:

Expression profiling by array

Platform:

GPL3681

9 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mycosarcoma maydis

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19655

18 Samples

Download data

(Submitter supplied) Goals: characterization of the transcription factor Ros1 in Ustilago maydis Methods: generation of deletion mutants, microscopic observations, ectopic expression of ros1, identification of Ros1 regulated genes by RNAseq and ChIP sequencing Results: Ros1 is not involved in plant colonization but is essential to trigger sporogenesis during late stages of infection. Premature expression of ros1 revealed that Ros1 counteracts the b-dependent filamentation program and induces morphological alterations resembling the early steps of sporogenesis. more...

Organism:

Mycosarcoma maydis

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19655

12 Samples

Download data: CSV

(Submitter supplied) Goals: characterization of the transcription factor Ros1 in Ustilago maydis Methods: generation of deletion mutants, microscopic observations, ectopic expression of ros1, identification of Ros1 regulated genes by RNAseq and ChIP sequencing Results: Ros1 is not involved in plant colonization but is essential to trigger sporogenesis during late stages of infection. Premature expression of ros1 revealed that Ros1 counteracts the b-dependent filamentation program and induces morphological alterations resembling the early steps of sporogenesis. more...

Organism:

Mycosarcoma maydis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19655

6 Samples

Download data: CSV, TXT

(Submitter supplied) The basidiomycete fungus Ustilago maydis causes smut disease in maize and has become an important model for elucidating the strategies used for host colonization by biotrophic fungi. In this study, we performed an in-depth transcriptional profiling of the plant-associated development of a cross between U. maydis FB1 and FB2 wildtype strains. The analysis of eight different stages, including the development on the leaf surface, early colonization, tumor induction and spore maturation, offers an unprecedented view of the changes in the fungal transcriptome associated with the passage through the entirely biotrophic life cycle. more...

Organism:

Zea mays; Mycosarcoma maydis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL19655 GPL24015 GPL15463

45 Samples

Download data: TXT

(Submitter supplied) The basidiomycete Ustilago maydis causes smut disease in maize. Colonization of the host plant is initiated by direct penetration of cuticle and cell wall of maize epidermis cells. The invading hyphae are surrounded by the plant plasma membrane and proliferate within the plant tissue. We identified a novel secreted protein, termed Pep1. Disruption mutants of pep1 are not affected in saprophytic growth and develop normal infection structures. more...

Organism:

Zea mays

Type:

Expression profiling by array

Platform:

GPL4032

6 Samples

Download data: CEL, CHP

(Submitter supplied) Infection-related development of phytopathogenic fungi is initiated by sensing and responding to plant surface cues. This response results in the formation of specialized infection structures, so-called appressoria. To unravel the program inducing appressoria in the biotrophic smut fungus Ustilago maydis, we exposed cells to a hydrophobic surface and the cutin monomer 16-hydroxy hexadecanoic acid. Genome-wide transcriptional profiling under these appressorium-inducing in vitro conditions revealed dramatic transcriptional changes in almost 20% of the genes. more...

Organism:

Mycosarcoma maydis

Type:

Expression profiling by array

Platform:

GPL3681

15 Samples

Download data: CEL

(Submitter supplied) Anthocyanin induction in plant is considered a general defense response against biotic and abiotic stresses. The infection by Ustilago maydis, the corn smut pathogen, is accompanied with anthocyanin induction in leaf tissue. We revealed that anthocyanin is intentionally induced by the virulence promoting secreted effector protein Tin2. Tin2 protein functions inside plant cells where it interacts with cytoplasmic maize protein kinase ZmTTK1. more...

Organism:

Zea mays

Type:

Expression profiling by array

Platform:

GPL4032

9 Samples

Download data: CEL

(Submitter supplied) Ustilago maydis is a biotrophic fungus that causes tumor formation on all aerial parts of maize. U. maydis secretes effector proteins during penetration and colonization to successfully overcome the plant immune response and reprogram host physiology to promote infection. In this study, we functionally characterized the U. maydis effector protein Topless (TPL) interacting protein 6 (Tip6). We found that Tip6 interacts with the N-terminus of RELK2 through its two Ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motifs. more...

Organism:

Zea mays

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25410

9 Samples

Download data: TXT

(Submitter supplied) We show that combinations of organic acids and glucose trigger phenotypes associated with the late stage of biotrophy for the maize pathogen Ustilago maydis. These phenotypes include the expression of a set of effectors normally observed only during biotrophic development as well as the formation of melanin associated with sporulation in plant tumors.

Organism:

Mycosarcoma maydis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31263

18 Samples

Download data: CSV

(Submitter supplied) Ustilago maydis is a basidiomycete fungus that causes smut disease in maize. Most prominent symptoms of the disease are plant tumors, which can be induced by U. maydis on all aerial parts of the plant. We identified two linked genes, pit1 and pit2, which are specifically expressed during plant colonization. Deletion mutants for either pit1 or pit2 are unable to induce tumor development and elicit plant defense responses. more...

Organism:

Zea mays

Type:

Expression profiling by array

Platform:

GPL4032

6 Samples

Download data: CEL, CHP

(Submitter supplied) The biotrophic fungus Ustilago maydis causes smut disease on maize (Zea mays L.), which is characterized by immense plant tumours. To establish disease and reprogram organ primordia to tumours, U. maydis deploys effector proteins in an organ-specific manner. However, the cellular contribution to leaf tumours remains unknown. We investigated leaf tumour formation on the tissue- and cell type-specific level. more...

Organism:

Zea mays; Mycosarcoma maydis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24163 GPL24165

5 Samples

Download data: DIFF, TSV

(Submitter supplied) We use this array to determine genes under the control of the transcriptional regulator Tup1

Organism:

Mycosarcoma maydis

Type:

Expression profiling by array

Platform:

GPL3681

4 Samples

Download data: CEL

(Submitter supplied) Study of gene regulation basidiocarps development in Ustilago maydis using transcriptomic analysis. In 2012, Cabrera-Ponce et al. established conditions allowing a completely different developmental program in U. maydis when grown on solid medium containing Dicamba (synthetic auxin) in dual cultures with maize embryogenic calli.

Organism:

Mycosarcoma maydis

Type:

Expression profiling by array

Platform:

GPL22595

3 Samples

Download data: CALLS, PAIR