Similar studies for GEO DataSets (Select 200069339) - GEO DataSets

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Items: 20

Select item 2000693391.

Genome-wide identification of differentially expressed microRNAs in leaves and the developing head of four durum genotypes during water deficit stress

(Submitter supplied) Background: MicroRNAs are endogenous small noncoding RNAs that play critical roles in plant abiotic stress responses. The interaction between miRNA-mRNA targets and their regulatory pathways in response to water deficit stress has been investigated in many plant species. However, the miRNA transcriptome of durum wheat (Triticum turgidum L. ssp. durum) is poorly characterised, with little known about miRNA functions related to water deficit stress. more...

Organism:: Triticum turgidum subsp. durum

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL20257
96 Samples

Download data: TAB

Series

Accession:: GSE69339

ID:: 200069339

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Select item 2001529732.

Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the Water-Deficit and Heat Stress Response Network in Durum Wheat

(Submitter supplied) Water-deficit and heat stress negatively impact crop production. Mechanisms underlying the response of durum wheat to such stresses are not well understood. With the new durum wheat genome assembly, we conducted the first multi-omics analysis with next-generation sequencing, providing a comprehensive description of the durum wheat small RNAome (sRNAome), mRNA transcriptome, and degradome. Single and combined water-deficit and heat stress were applied to stress-tolerant and -sensitive Australian genotypes to study their response at multiple time-points during reproduction. more...

Organism:: Triticum turgidum subsp. durum

Type:: Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Other

Platforms:: GPL28728 GPL20257
136 Samples

Download data: XLSX

Series

Accession:: GSE152973

ID:: 200152973

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Select item 2001539323.

Multi-omics analysis of small RNA, transcriptome and degradome in T. turgidum – regulatory networks of grain development and abiotic stress response

(Submitter supplied) Crop reproduction is highly sensitive to water-deficit and heat stress. The molecular networks of stress adaptation and grain development in tetraploid wheat (T. turgidum durum) are not well understood. Small RNAs (sRNAs) are important epigenetic regulators connecting the transcriptional and post-transcriptional regulatory networks. This study presents the first multi-omics analysis of the sRNAome, transcriptome and degradome in T. more...

Organism:: Triticum turgidum subsp. durum

Type:: Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Other

Platforms:: GPL28728 GPL20257
134 Samples

Download data: XLSX

Series

Accession:: GSE153932

ID:: 200153932

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Select item 2000505244.

Identification and characterization of microRNAs in the flag leaf and developing seed of wheat (Triticum aestivum L.)

(Submitter supplied) Background: MicroRNAs regulate various biological processes in plants. Considerable data are available on miRNAs involved in the development of rice, maize and barley. In contrast, little is known about miRNAs and their functions in the development of wheat. In this study, five small RNA (sRNA) libraries from wheat seedlings, flag leaves, and developing seeds were developed and sequenced to identify miRNAs and understand their functions in wheat development. more...

Organism:: Triticum aestivum

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL17701
5 Samples

Download data: FA

Series

Accession:: GSE50524

ID:: 200050524

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Select item 2001620085.

Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat

(Submitter supplied) Water-deficit stress negatively affects wheat yield and quality. Abiotic stress on the parental plants during reproduction could have transgenerational effects on the progenies. Here we investigated the transgenerational influence of pre-anthesis water-deficit stress by detailed analysis of the yield components, grain quality traits, and physiological traits in durum wheat. Next-generation sequencing analysis profiled the small RNA-omics, mRNA transcriptomics, and mRNA degradomics in the progenies. more...

Organism:: Triticum turgidum subsp. durum

Type:: Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Other

Platforms:: GPL20257 GPL28728
24 Samples

Download data: XLSX

Series

Accession:: GSE162008

ID:: 200162008

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Select item 2000837386.

Deep sequencing of wheat sRNA transcriptome reveals distinct temporal expression pattern of miRNAs in response to heat, light and UV

(Submitter supplied) Understanding of plant adaptation to abiotic stresses has implications in plant breeding, especially in the context of climate change. MicroRNAs (miRNAs) and short interfering RNAs play a crucial role in gene regulation. Here, wheat plants were exposed to one of the following stresses: continuous light, heat or ultraviolet radiations over five consecutive days and, leaf tissues from three biological replicates were harvested at 0, 1, 2, 3, 7 and 10 days after treatment (DAT). more...

Organism:: Triticum aestivum

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL17701
72 Samples

Download data: XLS

Series

Accession:: GSE83738

ID:: 200083738

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Select item 2000682677.

Identification of conserved and species-specific microRNAs in tea plant (Camellia sinensis) by deep sequencing

(Submitter supplied) In this study, it is noticeable that 32 tea-specific miRNAs were confirmed on the base of genome survey, using deep sequencing and microarray hybridization, and many miRNAs might associate with secondary metabolites synthesis.

Organism:: Camellia sinensis

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL20107
1 Sample

Download data: TXT

Series

Accession:: GSE68267

ID:: 200068267

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Select item 2000617008.

Identification and characterization of novel maize miRNAs involved in different genetic background

(Submitter supplied) MicroRNAs (miRNAs) are a class of small, non-coding regulatory RNAs that regulate gene expression by guiding target mRNA cleavage or translational inhibition in plants and animals. At present there is relatively little information regarding the role of miRNAs in the response to drought stress in maize. In this study, two small RNA libraries were sequenced, and a total of 11,973,711 and 14,326,010 raw sequences were generated from growing leaves of drought-tolerant and drought-sensitive maize seedlings, respectively. more...

Organism:: Zea mays

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL15463
2 Samples

Download data: TXT

Series

Accession:: GSE61700

ID:: 200061700

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Select item 2000525999.

Identification and characterization of miRNAome in root, stem, leaf and tuber developmental stages of potato (Solanum tuberosum L.) by high-throughput sequencing

(Submitter supplied) Purpose: MicroRNAs (miRNAs) are ubiquitous components of endogenous plant transcriptome. miRNAs are small, single-stranded and ~21 nt long RNAs which regulate gene expression at the post-transcriptional level and are known to play essential roles in various aspects of plant development and growth. Previously, a number of miRNAs have been identified in potato through in silico analysis and deep sequencing approach. more...

Organism:: Solanum tuberosum

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL15787
7 Samples

Download data: TXT

Series

Accession:: GSE52599

ID:: 200052599

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Select item 20005054610.

Identification and comparative analysis of miRNAs and their targets associated with curd-forming capacity at high temperature in two broccoli genotypes

(Submitter supplied) We report miRNA expression profiles underlying curd-forming capacity at high temperature in two broccoli lines by high-throughput sequencing.

Organism:: Brassica oleracea var. italica

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL17675
2 Samples

Download data: TXT

Series

Accession:: GSE50546

ID:: 200050546

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Select item 20006579911.

Small RNA and degradome sequencing reveal complex roles of miRNAs and their targets in the developing grains of wheat (Triticum aestivum L.)

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Triticum aestivum

Type:: Non-coding RNA profiling by high throughput sequencing; Other

Platforms:: GPL17701 GPL15632
6 Samples

Download data: TXT

Series

Accession:: GSE65799

ID:: 200065799

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Select item 20006579812.

Degradome sequencing analysis in developing wheat grains

(Submitter supplied) We aimed to identify targets of miRNAs during wheat grain development by using degradome sequencing approach. Two degradome libraries were constructed from wheat grains.

Organism:: Triticum aestivum

Type:: Other

Platform:: GPL15632
2 Samples

Download data: TXT

Series

Accession:: GSE65798

ID:: 200065798

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Select item 20006579713.

Expression profiling of small RNAs in developing wheat grains

(Submitter supplied) We aimed to identify differentially expressed miRNAs during wheat grain development by using high-throughput sequencing approach. Four small RNA libraries were constructed from wheat grains collected at 7, 14, 21 and 28 days post anthesis (DPA). A total of 165 known miRNAs and 37 novel miRNAs were identified in four small RNA libraries. Moreover, a miRNA-like long hairpin locus was first identified to produce 21~22-nt phased siRNAs. more...

Organism:: Triticum aestivum

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL17701
4 Samples

Download data: TXT

Series

Accession:: GSE65797

ID:: 200065797

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Select item 20005324214.

A genome-wide perspective of miRNAome in response to high temperature, salinity and drought stresses in Brassica juncea (Czern) L.

(Submitter supplied) Purpose: To identify high temperature, sailinity and drought-responsive miRNAs at genome wide level in B. juncea var varuna. Results: In this study four small RNA libraries viz. B. juncea control (BJC), B. juncea high temperature stressed (BJH), B. juncea salinity stressed (BJS) and B. juncea drought stressed (BJD) were prepared and sequenced. With the help of UEA sRNA workbench software package 51 conserved miRNAs belonging to 30 miRNA families were identified. more...

Organism:: Brassica juncea

Type:: Expression profiling by high throughput sequencing

Platform:: GPL16439
4 Samples

Download data: TXT

Series

Accession:: GSE53242

ID:: 200053242

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Select item 20005348715.

A Comprehensive Genome-wide Study on Tissue- and Abiotic Stress-specific miRNAs in Triticum aestivum

(Submitter supplied) Purpose: To identify abiotic stress responsive and tissue specific miRNAs at genome wide level in wheat (Triticum aestivum) Results: Small RNA libraries were constructed from four tissues (root, shoot, mature leaf and spikelets) and three stress treatments of wheat seedlings (control, high temperature, salinity and water-deficit). A total of 59.5 million reads were obtained by high throughput sequencing of eight wheat libraries, of which 32.5 million reads were found to be unique. more...

Organism:: Triticum aestivum

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL15632
8 Samples

Download data: TXT

Series

Accession:: GSE53487

ID:: 200053487

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Select item 20002574716.

A Genome-Wide Characterization of New and Drought Stress Responsive MicroRNAs in Populus euphratica

(Submitter supplied) We present an efficient method to genome-wide discover new and drought stress responsive miRNAs in P. euphratica. High throughput sequencing of P. euphratica leaves found 197 conserved miRNAs between P. euphratica and Populus trichocarpa. Meanwhile, 189 new miRNAs which belonged to 120 families were identified, a large increasing to the number of P. euphratica miRNAs. Target prediction and degradome sequencing verification of 22 new and 21 conserved miRNA targets showed these targets were involved in multiple biological processes, including transcription regulation and response to stimulus. more...

Organism:: Populus euphratica

Type:: Expression profiling by high throughput sequencing

Platform:: GPL11262
2 Samples

Download data: FASTA, TXT

Series

Accession:: GSE25747

ID:: 200025747

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Select item 20002635717.

Genome-wide profiling of small RNAs in rice under abiotic stresses

(Submitter supplied) To identify novel miRNA and NAT-siRNAs that are associated with abiotic stresses in rice, we generated small RNA sequences from inflorescences from rice under control and under dought, salt, and cold stress treatments. Over 30 million reads were generated.

Organism:: Oryza sativa

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL9147
4 Samples

Download data: TXT

Series

Accession:: GSE26357

ID:: 200026357

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Select item 20003713418.

Uncovering small RNA-mediated responses to cold stress in a wheat thermosensitive genic male sterile line by deep sequencing (Degradome-Seq)

(Submitter supplied) The male sterility of thermosensitive genic male sterile (TGMS) lines of wheat (Triticum aestivum) is strictly controlled by temperature. The early phase of anther development is especially susceptible to cold stress. MicroRNAs (miRNA) play an important role in plant development and in responses to environmental stress. In this study, deep sequencing of small RNA (smRNA) libraries obtained from spike tissues of the TGMS line under cold and control conditions identified a total of 81 unique miRNA sequences from 30 families, and trans-acting small interfering RNAs (tasiRNAs) derived from two TAS3 genes. more...