Similar studies for GEO DataSets (Select 200066254) - GEO DataSets

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Items: 20

Select item 2000662541.

Frequent derepression of the mesenchymal transcription factor gene in acute myeloid leukemia (human)

(Submitter supplied) Bone marrow samples from normal adult male donors were collected into EDTA. Red cells were removed by ammonium chloride lysis. Leukocytes were washed in SM buffer and CD34+ cells were separated from CD34- cells using an AutoMACS device and anti-CD34 immunomagnetic beads (Miltenyi Biotec), according to manufacturer’s instructions. For mature cell populations, CD34- cells were FACS purified according to the following immunophenotypes, with 7-AAD used to exclude dead cells: Neutrophils: side scatter high CD15+ CD16+. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL5188
8 Samples

Download data: CEL, TXT

Series

Accession:: GSE66254

ID:: 200066254

PubMed Similar studies

Select item 2000662562.

Frequent derepression of the mesenchymal transcription factor gene in acute myeloid leukemia

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens; Mus musculus

Type:: Expression profiling by array

Platforms:: GPL6096 GPL5188
17 Samples

Download data: CEL

Series

Accession:: GSE66256

ID:: 200066256

PubMed Similar studies

Select item 2000662533.

Frequent derepression of the mesenchymal transcription factor gene in acute myeloid leukemia (mouse)

(Submitter supplied) Murine CD45.1+ CD117+ BM cells were co-transduced pairwise with retroviral vectors expressing Hoxa9, Meis1, Foxc1 or a control empty vector. Ninety-six hours later 10^6 drug resistant cells were transplanted into CD45.2+ irradiated congenic recipients. To investigate the consequences of FOXC1 expression on the transcriptome in murine AML, we performed exon array analysis using flow sorted CD117+Gr1+ leukemia cells recovered from sick mice (Hoxa9/control; Moxa9/Meis1; or Hoxa9/FOXC1 leukemias; 3 mice per cohort).

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6096
9 Samples

Download data: CEL, TXT

Series

Accession:: GSE66253

ID:: 200066253

PubMed Similar studies

Select item 2001596934.

Enhancer binding of RUNX1 and Groucho family repressor TLE3 is stabilized by FOXC1 to block differentiation in acute myeloid leukemia

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:: GPL24676 GPL18573
30 Samples

Download data: BW

Series

Accession:: GSE159693

ID:: 200159693

PubMed Full text in PMC Similar studies

Select item 2001596915.

Enhancer binding of RUNX1 and Groucho family repressor TLE3 is stabilized by FOXC1 to block differentiation in acute myeloid leukemia [ChIP-Seq]

(Submitter supplied) A differentiation block is the cardinal pathologic feature of acute myeloid leukaemia (AML) but the underlying mechanisms are incompletely understood. Despite absent expression in normal hematopoietic lineages, the Forkhead family transcription factor FOXC1, which is a critical regulator of normal mesenchymal and mesodermal differentiation, is highly expressed in ~20% of cases of AML where it confers a block to monocyte/macrophage lineage differentiation. more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platforms:: GPL18573 GPL24676
26 Samples

Download data: BW

Series

Accession:: GSE159691

ID:: 200159691

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001596906.

Enhancer binding of RUNX1 and Groucho family repressor TLE3 is stabilized by FOXC1 to block differentiation in acute myeloid leukemia [RNA-Seq]

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18573
4 Samples

Download data: BW

Series

Accession:: GSE159690

ID:: 200159690

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 2000974507.

Frequent derepression of the Iroquois homeobox gene IRX3 in human acute leukemia

(Submitter supplied) The Iroquois homeodomain transcription factor gene IRX3 is highly expressed in the developing nervous system, limb buds and heart. In adults, expression levels specify risk of obesity. We now report a significant functional role for IRX3 in human acute leukemia. While transcript levels are very low in normal human bone marrow cell populations, high level IRX3 expression is observed in ~30% of patients with acute myeloid leukemia (AML), ~50% of patients with T-acute lymphoblastic leukemia and ~20% of patients with B-acute lymphoblastic leukemia, typically in association with high levels of HOXA9. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL19057
6 Samples

Download data: TXT

Series

Accession:: GSE97450

ID:: 200097450

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001131918.

CTCF boundary remodels chromatin domain and drives aberrant HOX gene transcription in acute myeloid leukemia

(Submitter supplied) We analyzed RNA-seq, ATAC-seq, ChIP-seq and 4C-seq data to find that CTCF binding site located between HOXA7 and HOXA9 genes (CBS7/9) is critical for establishing and maintaining aberrant HOXA9-HOXA13 gene expression in AML. Disruption of the CBS7/9 boundary resulted in spreading of repressive H3K27me3 into the posterior active HOXA chromatin domain that subsequently impaired enhancer/promoter chromatin accessibility and disrupted ectopic long-range interactions among the posterior HOXA genes. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Other

Platforms:: GPL21290 GPL18573
16 Samples

Download data: BED, TXT

Series

Accession:: GSE113191

ID:: 200113191

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 2000557139.

TGIF1 is a negative regulator of MLL-rearranged acute myeloid leukemia

(Submitter supplied) The aim of the study was to investigate the role of TGIF1 in MLL-AF9 transformed cells Members of the TALE (Three-amino acid loop extension) family of atypical homeodomain-containing transcription factors are prominent downstream effectors of oncogenic fusion proteins generated from translocations involving the mixed lineage leukemia (MLL) gene. A particular well-characterized member of this protein family is MEIS1, which together with HOXA proteins, orchestrates a transcriptional program required for the maintenance of MLL-rearranged acute myeloid leukemia (AML). more...

Organism:: Mus musculus

Type:: Expression profiling by array

Dataset:: GDS5456
Platform:: GPL6246
6 Samples

Download data: CEL, TXT

Series

Accession:: GSE55713

ID:: 200055713

Select item 20005528710.

TGIF1 is a negative regulator of MLL-rearranged acute myeloid leukemias

(Submitter supplied) Members of the TALE (Three-amino acid loop extension) family of atypical homeodomain-containing transcription factors are prominent downstream effectors of oncogenic fusion proteins generated from translocations involving the mixed lineage leukemia (MLL) gene. A particular well-characterized member of this protein family is MEIS1, which together with HOXA proteins, orchestrates a transcriptional program required for the maintenance of MLL-rearranged acute myeloid leukemia (AML). more...

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platforms:: GPL11002 GPL13112
9 Samples

Download data: BW, TXT

Series

Accession:: GSE55287

ID:: 200055287

PubMed Similar studies SRA Run Selector

Select item 545611.

TGIF1-transduced MLL-AF9-transformed leukemic cells

Analysis of mixed lineage leukemia (MLL)-AF9 transformed cells (MAF9) transduced with TGF-β induced factor 1 (TGIF1). TGIF1 is a member of the TALE (three-amino-acid loop extension) family of homeodomain-containing transcription factors. Results provide insight into the role of TGIF1 in MAF9 cells.

Organism:: Mus musculus

Type:: Expression profiling by array, count, 2 protocol sets

Platform:: GPL6246
Series:: GSE55713
6 Samples

Download data: CEL

DataSet

Accession:: GDS5456

ID:: 5456

Select item 20006864312.

PBX3 cooperates with MEISI in causing rapid acute myeloid leukemia and recapitulates the core transcriptome of MLL-rearranged leukemia

(Submitter supplied) To investigate whether co-expression of PBX3/MEIS1 can mimic that of MLL-AF9, HOXA9/MEIS1 or HOXA9/PBX3 in inducing leukemogenesis, we conducted in vivo mouse bone marrow transplantation (BMT) assays. Briefly, normal mouse bone marrow (BM) progenitor (i.e., lineage negative; Lin-) cells collected from B6.SJL (CD45.1) donor mice (CD45.1) were retrovirally co-transduced with MSCVneo-MLL-AF9+MSCV-PIG (MLL-AF9), MSCVneo-HOXA9+MSCV-PIG (HOXA9), MSCVneo-HOXA9+MSCV-PIG-MEIS1 (HOXA9+MEIS1), MSCVneo-HOXA9+MSCV-PIG-PBX3 (HOXA9+PBX3), MSCV-PIG-PBX3+MSCVneo-MEIS1 (PBX3+MEIS1), MSCVneo+MSCV-PIG-PBX3 (PBX3) , MSCVneo+MSCV-PIG-MEIS1 (MEIS1), or MSCVneo+MSCV-PIG (normal control; NC). more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL16570
20 Samples

Download data: CEL

Series

Accession:: GSE68643

ID:: 200068643

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20006363813.

The IDH2 mutation cooperates with the NPM1 mutation to activate Hoxa9/Meis1 and hypoxia pathways in acute myeloid leukemia

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by array; Methylation profiling by high throughput sequencing

Platforms:: GPL13112 GPL6246
22 Samples

Download data: BED, CEL

Series

Accession:: GSE63638

ID:: 200063638

Select item 20006363514.

The IDH2 mutation cooperates with the NPM1 mutation to activate Hoxa9/Meis1 and hypoxia pathways in acute myeloid leukemia (MeDip-seq)

(Submitter supplied) Mutations in IDH1 and IDH2 are frequently observed in various cancers, including acute myeloid leukemia (AML). Mutant IDHs convert α-ketoglutarate (α-KG) to 2-hydroxyglutarate (2-HG), which dysregulates a set ofα-KG-dependent dioxygenases. To determine whether mutant IDHs are valid targets for cancer therapy, we established a mouse AML model harboring an IDH2 mutation by transplanting mice with nucleophosmin1 (NPM1)+/- mouse hematopoietic stem/progenitor cells that had been co-transduced with four mutant genes (NPMc, IDH2/R140Q, DNMT3A/R882H and FLT3/ITD) that frequently occur simultaneously in human AML patients. more...

Organism:: Mus musculus

Type:: Methylation profiling by high throughput sequencing

Platform:: GPL13112
3 Samples

Download data: BED

Series

Accession:: GSE63635

ID:: 200063635

PubMed Similar studies SRA Run Selector

Select item 20006361815.

The IDH2 mutation cooperates with the NPM1 mutation to activate Hoxa9/Meis1 and hypoxia pathways in acute myeloid leukemia (expression)

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
19 Samples

Download data: CEL

Series

Accession:: GSE63618

ID:: 200063618

Select item 20002794716.

Identification of differentially regulated genes in hematopoietic stem cells and URE leukemia cell line

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
12 Samples

Download data: CEL

Series

Accession:: GSE27947

ID:: 200027947

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20002793917.

Identification of differentially regulated genes upon shRNA-mediated knock-down of HLX in the URE leukemia cell line

(Submitter supplied) To study the role of Hlx in hematopoietic differentiation and tumorigenesis, URE cells were infected with short-hairpin-containing pSIH1-H1-copGFP lentiviral vector (System Biosciences, Mountain View, CA) containing either nucleotide sequences targeting luciferase (shControl) or HLX (shHLX). After 24hrs incubation in Iscove’s modified Dulbecco’s medium (IMDM) containing FBS, mIL-3, mIL-6 and mSCF with lentiviral supernatants in the presence of 8ug/ml polybrene, cells were cultured in fresh medium for several days. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
6 Samples

Download data: CEL

Series

Accession:: GSE27939

ID:: 200027939

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20002793818.

Identification of differentially regulated genes upon overexpression of HLX in wild-type hematopoietic stem cells

(Submitter supplied) The goal was to study the role of Hlx in hematopoiesis.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
6 Samples

Download data: CEL

Series

Accession:: GSE27938

ID:: 200027938

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20007527219.

The role of Hoxa9 and Meis1 in development of acute myeloid leukemia (mRNA)

(Submitter supplied) OBJECTIVE: MEIS1, a HOX cofactor, collaborates with multiple HOX proteins, such as HOXA9, to accelerate the onset of acute myeloid leukemia (AML) through largely unknown molecular mechanisms. To further resolve these mechanisms, we conducted a structure-function analysis of Meis1 and gene expression profiling, in the context of Hoxa9 leukemogenesis. RESULTS: We show, in a murine bone marrow transplantation model, that the homeodomain of Meis1 is required for leukemogenic collaboration with Hoxa9. more...