Similar studies for GEO DataSets (Select 200057246) - GEO DataSets

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Items: 2

Select item 2000572461.

Array CGH data of A. oryzae strains bearing targeted tandem chromosomal duplications

(Submitter supplied) We here describe the first successful construction of a targeted tandem duplication of a large chromosomal segment in Aspergillus oryzae. The targeted tandem chromosomal duplication was achieved by using strains that had 5’ΔpyrG upstream of the region targeted for tandem chromosomal duplication and 3’ΔpyrG downstream of the target region. Consequently, strains bearing a 210-kb targeted tandem chromosomal duplication near the centromeric region of chromosome 8 and strains bearing a targeted tandem chromosomal duplication of a 700-kb region of chromosome 2 were successfully constructed. more...

Organism:: Aspergillus oryzae RIB40

Type:: Genome variation profiling by array

Platform:: GPL16017
2 Samples

Download data: TXT

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Accession:: GSE57246

ID:: 200057246

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Select item 2001206042.

Array CGH data of A. oryzae strains bearing translocated chromosomal duplication and triplication

(Submitter supplied) In this study, we achieved translocated chromosomal duplication and triplication of a 1.4-Mb targeted chromosomal region by directly introducing I-SceI meganuclease into A. oryzae protoplast cells. Strains with duplication and triplication of chromosome 2 showed substantial increases in the activity of protease and amylase. Gene dosage effects were enhanced by combining the structural gene and its regulatory gene, indicating that segmental duplications of chromosomes play important phenotypic roles in koji mold strains.