GEO DataSets

(Submitter supplied) T cell anergy is one of the mechanisms contributing to peripheral tolerance, particularly in the context of progressively growing tumors and in tolerogenic treatments promoting allograft acceptance. We recently reported that early growth response gene 2 (Egr2) is a critical transcription factor for the induction of anergy in vitro and in vivo, which was identified based on its ability to regulate the expression of inhibitory signaling molecules diacylglycerol kinase (DGK)-a and -z. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by array

Platforms:

GPL9185 GPL1261

15 Samples

Download data: BED, CEL, CHP

(Submitter supplied) We report the application of sequencing technology for mapping the EGR2 transcriptional program in T cell anergy

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9185

3 Samples

Download data: BED

(Submitter supplied) T cell receptor(TCR) engagement in the absence of costimulation leads to a state of T cell tolerance known as anergy. Anergy induction requires new protein synthesis since it is inhibited by cycloheximide. In this experiment, we tried to figure out kinetic properties of the gene expression in anergy induction phase and the subsequent anergy maintenance phase.

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL83 GPL81 GPL82

15 Samples

Download data: CEL

(Submitter supplied) EGR2 is an early growth response transcription factor that negatively regulates T-cell activation, in contrast to its positive regulation by EGR1. Here, we unexpectedly found that EGR2 promotes peripheral naïve T cell differentiation, with delayed TCR-induced proliferation in naïve T cells from Egr2 conditional knockout (CKO) mice, and decreased production of IFN-γ, IL-4, IL-9, and IL-17A in cells subjected to T helper differentiation. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

12 Samples

Download data: BED, TXT

(Submitter supplied) pooled mRNA (2, 4, 6 hrs) from A.E7 T cells under conditions which promote or inhibit anergy induction Keywords: ordered

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS1336 GDS1338 GDS1339

Platforms:

GPL82 GPL83 GPL81

15 Samples

Download data

Analysis of pooled mRNA (2, 4, 6 hours) from A.E7 T cell clones activated in conditions that promote (anti-TCR) or inhibit (anti-TCR + CsA or PKCi or Combo) anergy induction. Results identify the early growth response gene 2 (Egr-2) and Egr-3 as key positive regulators of T cell anergy induction.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 5 agent, 3 protocol sets

Platform:

GPL83

Series:

GSE2323

5 Samples

Download data

Analysis of pooled mRNA (2, 4, 6 hours) from A.E7 T cell clones activated in conditions that promote (anti-TCR) or inhibit (anti-TCR + CsA or PKCi or Combo) anergy induction. Results identify the early growth response gene 2 (Egr-2) and Egr-3 as key positive regulators of T cell anergy induction.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 5 agent, 3 protocol sets

Platform:

GPL82

Series:

GSE2323

5 Samples

Download data

Analysis of pooled mRNA (2, 4, 6 hours) from A.E7 T cell clones activated in conditions that promote (anti-TCR) or inhibit (anti-TCR + CsA or PKCi or Combo) anergy induction. Results identify the early growth response gene 2 (Egr-2) and Egr-3 as key positive regulators of T cell anergy induction.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 5 agent, 3 protocol sets

Platform:

GPL81

Series:

GSE2323

5 Samples

Download data

(Submitter supplied) compare gene expression profiles between normal and anergic T cells and identify upregulated genes in anergic T cells Keywords: cell type comparison

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2400

Platform:

GPL81

4 Samples

Download data

Analysis of T cells anergized in vitro with plate-bound anti-CD3. Anergy is a hyporesponsive state resulting from the engagement of the T cell receptor in the absence of CD28 costimulation. Results provide insight into the mechanistic basis of T cell anergy.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type sets

Platform:

GPL81

Series:

GSE5960

4 Samples

Download data

(Submitter supplied) In this study, we examined the role of the transcriptional regulator EGR2 in CD8+ T cell exhaustion during chronic viral infection. We conducted scRNAseq analysis on sorted virus-specific tetramer+ CD8+ T cells isolated at day 20 post-infection with chronic LCMV-Cl13 from either littermate control or EGR2 T cell conditional knock-out mice. Cells were labelled with hashtags and mixed prior to multiplexed analysis. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24247 GPL19057

2 Samples

Download data: H5

(Submitter supplied) In this study, we examined the role of the transcriptional regulator EGR2 in CD8+ T cell exhaustion during chronic viral infection. Flow cytometric and scRNAseq analysis indicated that EGR2 deficient CD8+ T cells had an abnormal effector-like phenotype. To examine whether this was due to epigenetic alterations, we conducted ATACseq analysis on sorted virus-specific tetramer+ CD8+ T cells isolated at day 20 post-infection with chronic LCMV-Cl13 from either littermate control or EGR2 T cell conditional knock-out mice. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

4 Samples

Download data: BW

(Submitter supplied) In this study, we examined the role of the transcriptional regulator EGR2 in CD8+ T cell exhaustion during chronic viral infection. Flow cytometric analysis indicated that EGR2 is expressed selectively within the progenitor exhausted subset, however a subpopulation of progenitor exhausted cells did not express in EGR2. To define the differences between the EGR2+ and EGR2- progenitor exhausted cells, GFP+ and GFP- polyclonal progenitor exhausted (ie. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

4 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL19057 GPL24247

16 Samples

Download data: BW, H5, NARROWPEAK

(Submitter supplied) In this study, we examined the role of the transcriptional regulator EGR2 in CD8+ T cell exhaustion during chronic viral infection. Flow cytometric and RNAseq analysis indicated that EGR2 deficient CD8+ T cells had a block in differentiation and failed to undergo terminal exhaustion. To examine the direct gene targets of EGR2 during exhaustion, we conducted ChIPseq analysis on enriched bulk splenic CD8+ T cells isolated at day 20 post-infection with chronic LCMV-Cl13 from C57BL/6J mice. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

2 Samples

Download data: NARROWPEAK

(Submitter supplied) In this study, we examined the role of the transcriptional regulator EGR2 in CD8+ T cell exhaustion during chronic viral infection. Flow cytometric analysis indicated that EGR2 deficient CD8+ T cells had a block in differentiation and failed to undergo terminal exhaustion. To confirm this at a whole transcriptome level, we conducted RNAseq analysis on sorted splenic virus-specific tetramer+ CD8+ T cells isolated at day 20 post-infection with chronic LCMV-Cl13 from either littermate control or EGR2 T cell conditional knock-out mice. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

4 Samples

Download data: CSV

(Submitter supplied) Unraveling the molecular mechanisms of NK cell dysfunction and implications for anti-tumor immunotherapy.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

8 Samples

Download data: TXT

(Submitter supplied) IL-10 is an immunomodulant cytokine that plays a central role in controlling inflammation, down-regulating immune responses, and inducing immunological tolerance. IL-10 inhibits the expression of costimulatory molecules and the secretion of inflammatory cytokines by antigen-presenting cells (APC), directly suppresses T-cell proliferation, and promotes T-cell anergy. In this study we demonstrate that IL-10 inhibits primary allogeneic proliferation in total PBMC and induces antigen(Ag)-specific T-cell hyporesponsiveness. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

12 Samples

Download data: TXT

(Submitter supplied) Genetic engineering of hematopoietic cells ex-vivo opens new opportunities for programming immune cells and improving their functionality in response to illness and infections. Molecular methods for genetic manipulation are constantly improving, but it is challenging to reveal which genes are the most beneficial to target and whether multiple genes tackling can further improve the desired outcome. Current screening methods are limited by scale, the number of perturbations, and low content readouts. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL24247

16 Samples

Download data: TAR