GEO DataSets

(Submitter supplied) In adult skin, each hair follicle contains a reservoir of stem cells (the bulge), which can be mobilized to regenerate the new follicle with each hair cycle and to reepithelialize epidermis during wound repair. Here we report new methods that permit their clonal analyses and engraftment and demonstrate the two defining features of stem cells, namely self-renewal and multi-potency. We also show that, within the bulge, there are two distinct populations, one of which maintains basal lamina contact and temporally precedes the other, which is suprabasal and arises only after the start of the first postnatal hair cycle. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL339

4 Samples

Download data: CEL

(Submitter supplied) To assess if Hedgehog (Hh) responding cells in the skin have a unique expression profile, isolated keratinocytes that express the Hh response gene Gli1 were collected by FACS and their gene expression was compared to sorted CD34-expressing cells from the middle bulge region of the hair follicle and to cells from the interfollicular epidermis (IFE) by hybridization of isolated RNA to gene expression microarrays.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

8 Samples

Download data: TXT

(Submitter supplied) The goal of this study was to identify transcripts that were differentially regulated in Lgr5 hair follicle stem cells following skin injury in the presence and absence of regulatory T cells

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

8 Samples

Download data: CSV

(Submitter supplied) Thymic epithelial cells (TECs) are essential for thymopoiesis and form a complex three-dimensional network, the organization of which is strikingly different from other epithelia. Interestingly, TECs express simple epithelia keratins in the cortex, stratified epithelia keratins in the medulla and epidermal differentiation markers in Hassall's bodies. Here we investigate the relationship between thymic epithelium and epidermal differentiation and show that the thymus of the rat contains a population of clonogenic TECs that can be extensively cultured and cloned using conditions developed for epidermal cell therapy in human. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL6247

32 Samples

Download data: CEL

(Submitter supplied) Mouse keratinocytes were isolated from K15-EGFP transgenic mice for FACS sorting. RNA samples from EGFP-high and alpha-6 integrin positive cells (hair follicle stem cells) and from EGFP negative and alpha-6 integrin positive cells were used for Microarray analysis. Keywords = stem cells Keywords = hair follicle Keywords = epidermis Keywords = alopecia Keywords: ordered

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS840

Platform:

GPL81

6 Samples

Download data: CEL

Analysis of hair follicle stem cells (FACS-isolated Keratin1-15-EGFP-positive keratinocytes) from bulge cell population of 50-60 day old female B6SJL/F1 transgenics. Results provide targets for the treatment of hair loss and other disorders of skin and hair.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type sets

Platform:

GPL81

Series:

GSE1096

6 Samples

Download data: CEL

(Submitter supplied) In mammals, organ induction occurs only during embryonic development except for hair follicles (HFs). However, HF-resident epithelial stem cells (HFSCs), which are responsible for repetitive HF regeneration, are not fully characterized. We establish in vitro culture systems that are capable of controlling the ability of HFSCs to regenerate HFs and perform microarray analysis to compare gene expression profile in different culture condition.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

(Submitter supplied) Expression of deltaNB-cateninER allows the time and duration of B-catenin activation to be controlled precisely through the application of 4OHT. We have shown that B-catenin activation in adult mouse epidermis stimulates de novo hair follicles and the formation of a new bulge and melanocyte niche. We utilize Affymetrix Mouse Genome 430A 2.0 oligonucleotide arrays to investigate the differential regulation of RNA in skin from 7 week old female deltaNB-cateninER transgenic mice following 0, 1, or 7 days B-catenin activation in the epidermis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1560

Platform:

GPL339

16 Samples

Download data

Analysis of skin of deltaNB-cateninER transgenics following the activation of beta-catenin for up to 7 days. Onset and duration of beta-catenin activation in deltaNB-cateninER transgenics controlled by 4-hydroxytamoxifen. Results provide insight into how beta-catenin induces hair follicle growth.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 strain, 3 time sets

Platform:

GPL339

Series:

GSE1579

16 Samples

Download data

(Submitter supplied) Foxi3 is a transcription factor expressed in the hair follicle epithelium during development and postnatally. In this study we used a microarray analysis to indentify differentially expressed genes in Foxi3 null epithelium compared to Foxi3 wt epithelium. We used E15.5 stage as the earliest time point when the Foxi3 null hair phenotype bacame obvious, to find out the most early consequences of Foxi3 ablation.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL19752

8 Samples

Download data: CEL, TXT

(Submitter supplied) Mammalian epidermis consists of three self-renewing compartments: the hair follicle, sebaceous gland and interfollicular epidermis. We generated knock-in alleles of murine Lgr6, a close relative to the Lgr5 stem cell gene. Lgr6 was expressed in the earliest embryonic hair placodes. In adult hair follicles, Lgr6+ cells resided in a previously uncharacterized region directly above the follicle bulge. They expressed none of the known bulge stem cell markers. Prenatal Lgr6+ cells established the hair follicle, sebaceous gland and interfollicular epidermis. Postnatally, Lgr6+ cells generated sebaceous gland and interfollicular epidermis, while contribution to hair lineages gradually diminished with age. Adult Lgr6+ cells executed long-term wound repair, including the formation of new hair follicles. We conclude that Lgr6 marks the most primitive epidermal stem cell.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

2 Samples

Download data: TXT

(Submitter supplied) We isolated murine sebaceous gland (SG) cells and performed highly targeted single cell RNA-sequencing (scRNA-seq) to identify novel cell subpopulations and to infer lineage relationships.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

4 Samples

Download data: MTX, RDATA, TSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

17 Samples

Download data

(Submitter supplied) In-depth transcriptional analysis of mature hair placode and interfollicular epithelium using scRNA-seq method.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

3 Samples

Download data: TXT

(Submitter supplied) In-depth transcriptional analysis of mature hair placode and interfollicular epithelium using bulk RNA-seq method.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

14 Samples

Download data: TXT

(Submitter supplied) The purpose of this study was to define, at the single cell level, the transcriptionnal profile of murine tail epidermal basal cells during potsnatal growth and to compare with adult homeostatic basal cells. The analysis was performed on Lgr5DTR-EGFP mice (Tian et al., 2011)(knockin mice containing an Enhanced Green Fluorescent Protein (EGFP) under the control of the Lgr5 regulatory region), allowing to identify and exclude Lgr5-expressing cells of the bulge and basal cells of the interfollicular epidermis were enriched using EGFP negative, CD34 negative, alpha6 integrin positive gating. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

3 Samples

Download data: CSV, MTX, RDS, TSV

(Submitter supplied) SMARTseq2 scRNAseq of interfollicular epidermis basal cells at P20

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

188 Samples

Download data: CSV

(Submitter supplied) Purpose: The goal of this study is to characterise cortical (cTECs) and medullary (mTECs) epithelial cells of the human thymus. Methods: FACS-isolation protocol to separate 4 different epithelial cells populations from total dissociation of human thymus that is negative for hCD45 and either positive for EPCAM or CD205, then loaded into the 10x Genomics Chromium Platform, and sequenced using Illumina HiSeq 4000. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20301

4 Samples

Download data: TSV

(Submitter supplied) Purpose: The goal of this study is to characterise expanding thymic progenitor and stem cells from whole epithelial as well as cortical, medullary thymus sub-compartments. As reference, we have sequenced skin keratinocyte progenitor and stem cells Methods: Cells have been expanded for 4 passages on mouse feeder layer cells then loaded into the 10x Genomics Chromium Platform, and sequenced using Illumina HiSeq 4000. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20301

7 Samples

Download data: TSV

(Submitter supplied) Purpose: The goal of this study is to characterise expanding epithelial progenitor and stem cells in culture from different organs and compare them to thymic ones Methods: FACS-isolation protocol to separate epithelial cells from mouse feeder layer cells then loaded into the 10x Genomics Chromium Platform, and sequenced using Illumina HiSeq 4000. Conclusions: Our study dissected in vitro properties of airways and skin progenitor and stem cells and showed they differ from thymic ones

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20301

2 Samples

Download data: TSV