GEO DataSets

(Submitter supplied) Transactive response DNA-binding protein of 43 kDa (TDP-43), a heterogeneous nuclear ribonucleoprotein (hnRNP) with diverse activities, is a common denominator in several neurodegenerative disorders including amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Orthologs of TDP-43 exist from mammals to invertebrates, but their functions in lower organisms remain poorly understood. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS4573

Platform:

GPL200

6 Samples

Download data: CEL

Analysis of tdp-1(ok803lf) mutants. TDP-1 loss alleviates proteotoxicity and extends life span. Results provide insight into a role for TDP-1 in the regulation of protein homeostasis and aging.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL200

Series:

GSE34113

6 Samples

Download data: CEL

(Submitter supplied) Purpose: We used RNA-Seq to compare the gene expression profiles in two long-lived C. elegans strains: (1) animals with a loss-of-function mutation in the hsb-1 gene, and (2) animals overexpressing hsf-1 under its endogenous promoter. Previous studies indicated that life span extension in both these strains is hsf-1-dependent. Methods: mRNA-Seq profiles for three biological replicates each of day 1 adult wild-type (N2 strain), hsb-1 mutant and hsf-1 overexpression strains were generated using 50 bp single-end sequencing on an Illumina HiSeq 2500 platform. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18245

9 Samples

Download data: TXT

(Submitter supplied) FoxO transcription factors promote longevity across taxa. How they do so is poorly understood. In the nematode Caenorhabditis elegans, the A- and F-isoforms of the FoxO transcription factor DAF-16 extend life span in the context of reduced DAF-2 insulin-like growth factor receptor (IGFR) signaling. To elucidate the mechanistic basis for DAF-16/FoxO-dependent life span extension, we performed an integrative analysis of isoform-specific daf-16/FoxO mutants. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13657

21 Samples

Download data: TXT

(Submitter supplied) The gene expression profile of WT (N2) C. elegans were compared to that of ets-4(ok165) and ets-4(uz1) mutant animals

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS3747

Platform:

GPL7272

6 Samples

Download data: TXT

Analysis of stage L4 larvae with two distinct deletion alleles of ets-4: ets-4(ok165) and ets-4(uz1). Adults with ets-4 mutations have a significant extension of mean life span. Results provide insight into the transcriptional control of aging effectors by the ETS-4 transcription factor.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, log2 ratio, 2 genotype/variation sets

Platform:

GPL7272

Series:

GSE21851

6 Samples

Download data: TXT

(Submitter supplied) TDP-1 is the C. elegans ortholog of mammalian TDP-43, which is strongly implicated in the etiology of Frontotemporal Dementia (FTD) and Amyotrophic Lateral Sclerosis (ALS). We discovered that deletion of the tdp-1 gene results in enhanced transcriptional gene silencing leading to increased sensitivity to heritable RNA interference (RNAi). As heritable RNAi in C. elegans depends on chromatin changes moderated by HPL-2, a homolog of heterochromatin protein 1 (HP1), we investigated the interaction of TDP-1 and HPL-2. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Other

Platforms:

GPL13657 GPL18245

21 Samples

Download data: BW, TXT, XLSX

(Submitter supplied) Heat shock factor 1 (HSF-1) and forkhead box O (FOXO) are key transcription factors that protect cells from various stresses. InCaenorhabditis elegans, HSF-1 and FOXO together promote a long life span when insulin/IGF-1 signaling (IIS) is reduced. However, it remains poorly understood how HSF-1 and FOXO cooperate to confer IIS-mediated longevity. Here, we show that prefoldin 6 (PFD-6), a component of the molecular chaperone prefoldin-like complex, relays longevity response from HSF-1 to FOXO under reduced IIS. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18245

12 Samples

Download data: TXT

(Submitter supplied) Analysis of gene expression in long-lived daf-2 mutants for the insulin/IGF-1 receptor. daf-2(e1370) and daf-2(m577) alleles each examined as single and as double mutations with the daf-16(df50) allele. DAF-2 regulates DAF-16, a transcription factor. Results provide insight into longevity. Keywords: other

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS770

Platform:

GPL200

20 Samples

Download data

Analysis of gene expression in long-lived daf-2 mutants for the insulin/IGF-1 receptor. daf-2(e1370) and daf-2(m577) alleles each examined as single and as double mutations with the daf-16(df50) allele. DAF-2 regulates DAF-16, a transcription factor. Results provide insight into longevity.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, transformed count, 4 genotype/variation sets

Platform:

GPL200

Series:

GSE1762

20 Samples

Download data

(Submitter supplied) One of the most important issues in the study of aging is to discover compounds with longevity-promoting activity and to unravel their underlying mechanisms. Queen honey bees are continuously fed royal jelly (RJ), and they live more than 10 times longer than hive workers, derived from the same diploid genome, which are fed it only for a short period of time during their larval stages. Therefore, RJ is likely to contain longevity-promoting agents for queens. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL11346

6 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of whole day 1 adult C. elegans, comparing animals carrying the m79 mutation in the daf-10 gene and wild-type isogenic animals. Genes that act downstream of sensory neurons to influence longevity, dauer formation and pathogen responses in Caenorhabditis elegans Manuscript abstract:

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL9450

3 Samples

Download data: GPR

(Submitter supplied) Many studies have addressed the effect of dietary glycemic index on obesity and diabetes, but little is known about its effect on lifespan itself. We found that adding a small amount of glucose to the medium (0.1-2%) shortened the lifespan of C. elegans. Glucose shortened lifespan by inhibiting the activities of lifespan-extending transcription factors that are also inhibited by insulin signaling: the FOXO family member DAF-16 and the heat shock factor HSF-1. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL9450

14 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of adult C. elegans comparing normally cultured control animals with adult animals that were cultured on diet supplemented with 2% D-glucose.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL9450

6 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of adult C. elegans comparing daf-16(-); daf-2(-) animals with adult daf-2(-) animals.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL9450

8 Samples

Download data: GPR

(Submitter supplied) To mitigate the deleterious effects of temperature increases on cellular organization and proteotoxicity, organisms have developed mechanisms to respond to heat stress. In eukaryotes, HSF1 is the master regulator of the heat shock transcriptional response, but the heat shock response pathway is not yet fully understood. From a forward genetic screen for suppressors of heat shock induced gene expression in Caenorhabditis elegans, we found a new allele of hsf-1 that alters its DNA-binding domain, and we found three additional alleles of sup-45, a previously molecularly uncharacterized genetic locus. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18245

12 Samples

Download data: TSV

(Submitter supplied) C. elegans mutants deleted for TDP-1, an ortholog of the neurodegeneration-associated RNA binding protein TDP-43, display only mild phenotypes. Nevertheless, transcriptome sequencing revealed that many RNAs were altered in accumulation and/or processing in the mutant. Analysis of these transcriptional abnormalities demonstrates that a primary function of TDP-1 is to limit formation or stability of double-stranded RNA. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13657

24 Samples

Download data: BED, TXT

(Submitter supplied) We observed that heat shock of Caenorhabditis elegans leads to the formation of nuclear double-stranded RNA (dsRNA) foci, detectable with a dsRNA-specific monoclonal antibody. These foci significantly overlap with nuclear HSF-1 granules. To investigate the molecular mechanism(s) underlying dsRNA foci formation, we used RNA-seq to globally characterize total RNA and immunoprecipitated dsRNA from control and heat-shocked worms. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13657

11 Samples

Download data: TXT

(Submitter supplied) Acyl-coA synthases (ACSs) produce fatty acyl-CoAs that are used in metabolic and signaling pathways. Metazoans have a large number of ACS genes with differing expression patterns and substrate preferences, but the physiological roles of most ACS genes are unknown. Here, we focused on the C. elegans acyl CoA synthase, ACS-3, which is known to regulate fat uptake and de novo fat synthesis through the conserved nuclear hormone receptor, nhr-25. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL5859

6 Samples

Download data: GPR

(Submitter supplied) To determine how gene expression changes in long-lived ash-2-deficient worms expressing the amyloidogenic Q40::YFP, and to assess how simultaneous depletion of hsf-1 effects these transcriptomic changes.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26672

17 Samples

Download data: XLSX