GEO DataSets

(Submitter supplied) Bacillus subtilis is exposed to a wide range of transitory stress and starvation conditions. Here we investigate the expression changes observed in the B. subtilis wild type strain 168 and its isogenic sigB mutant(BSM29) with respect to each stress condition tested.

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL14719

111 Samples

Download data: TXT

(Submitter supplied) The alternative sigma factor sigmaB has an important role in the acquisition of stress-resistance in many gram-positive bacteria. In the foodborne pathogen Bacillus cereus sigmaB is activated strongly upon a heat shock and other stress conditions. Here we describe the identification of the set of sigmaB-regulated genes in B. cereus by DNA microarray analysis of the transcriptome upon a mild heat shock. more...

Organism:

Bacillus cereus ATCC 14579

Type:

Expression profiling by array

Platform:

GPL4406

7 Samples

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(Submitter supplied) Comparing the transcriptional responses of Bacillus subtilis strains WN624 and WN1106 at 5 kPa and 101 kPa. WN1106 is a 5 kPa-evolved strain with increased fitness compared to ancestor-WN624 strain at 5 kPa. This experiment probed the difference in response when the strains are grown at 5 kPa.

Organism:

Bacillus subtilis subsp. subtilis str. 168

Type:

Expression profiling by array

Platform:

GPL17693

8 Samples

Download data: TXT

(Submitter supplied) Background: The Bacillus cereus Sigma B (SigB) dependent general stress response is activated via the two-component RsbKY system, which involves a phosphate transfer from RsbK to RsbY. It has been hypothesized that the Hpr-like phosphocarrier protein (Bc1009) encoded by bc1009 in the SigB gene cluster may play a role in this transfer, thereby acting as a regulator of SigB activation. Alternatively, Bc1009 may be involved in the activation of a subset of SigB regulon members. more...

Organism:

Bacillus cereus ATCC 14579

Type:

Expression profiling by array

Platform:

GPL9493

16 Samples

Download data: TXT

(Submitter supplied) Whole-genome microarray technology and state-of-the-art proteomic techniques were applied to provide a global and time-resolved picture of the physiological response of B. subtilis cells exposed to a severe and sudden osmotic up-shift. This combined experimental approach provided quantitative data for 3961 mRNA profiles, 590 expression profiles of proteins detected in the cytosol and 383 expression profiles of proteins detected in the membrane fraction. more...

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL3502

15 Samples

Download data: CSV

(Submitter supplied) Bacillus subtilis responds to phosphate starvation stress by inducing the PhoP and SigB regulons. While the PhoP regulon provides a specific response to phosphate starvation stress, maximizing the acquisition of phosphate (Pi) from the environment and reducing the cellular requirement for this essential nutrient, the SigB regulon provide non-specific resistance to stress by protecting essential cellular components such as DNA and membranes. more...

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL188

39 Samples

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(Submitter supplied) Abstract of associated manuscript: The Bacillus subtilis extracytoplasmic function (ECF) sigma(M) factor is activated by cell envelope stress elicited by antibiotics, and by acid, heat, ethanol and superoxide stresses. Here, we have used several complementary approaches to identify genes controlled by sigma(M). In many cases, expression is only partially dependent on sigma(M) because of both overlapping promoter recognition with other ECF sigma factors and the presence of additional promoter elements. more...

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL7420

14 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL2760

78 Samples

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(Submitter supplied) Comparison between perR and wild type Bacillus Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL2760

6 Samples

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(Submitter supplied) Bacillus subtilis exhibits a complex adaptive response to low levels of peroxides. We used global transcriptional profiling to monitor the magnitude and kinetics of changes in the mRNA population after exposure to either hydrogen peroxide (H2O2) or tert-butyl peroxide (t-buOOH). The peroxide stimulons could be largely accounted for by three regulons controlled by the PerR, B, and OhrR transcription factors. more...

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL2760

72 Samples

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(Submitter supplied) In previously published work, we identified three Mycobacterium tuberculosis sigma (s) factor genes responding to heat shock (sigB, sigE and sigH ). Two of them (sigB and sigE ) also responded to SDS exposure. As these responses to stress suggested that the s factors encoded by these genes could be involved in pathogenicity, we are studying their role in physiology and virulence. In this work, we characterize a sigE mutant of M. more...

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by array

Platform:

GPL2787

15 Samples

Download data: TXT

(Submitter supplied) Comprison of the transcriptome of wild type Bacillus subtilis to the mutants sigW, rasP and prsW to select all candidate sigW regulated genes. We identified 89 genes as being sigW regulated, including several non-coding RNA's. The effects of rasP and prsW mutations on sigW regulated genes were relatively mild, implying that RasP or PrsW are not strictly required for sigW activation. The rasP mutant has a peliotropic phenotype, affecting competence development, protein secretion and membrane protein production. more...

Organism:

Bacillus subtilis subsp. subtilis str. 168

Type:

Expression profiling by genome tiling array

Platform:

GPL15150

11 Samples

Download data: PAIR

(Submitter supplied) Genome-wide comparative transcriptome analysis of the Bacillus subtilis parent strain PY79 and its yvfI derivative with yvfI::T10::spc (TEK1) grown in PA medium Detailed description of sample preparation and microarray conditions can be also found in Irigul et al to be submitted

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL6031

3 Samples

Download data: TXT

(Submitter supplied) In Gram-positive organisms the general stress response is regulated by the alternative sigma factor sigma B (σB). In C. difficile this sigma factor has been implicated in protection against stressors such as reactive oxygen species and antimicrobial compounds. Here, we show that an anti-σB antibody can be used to demonstrate overexpression of σB in C. difficile and that this overexpression is tolerated for short periods of time, despite its toxicity. more...

Organism:

Clostridioides difficile 630

Type:

Expression profiling by array

Platform:

GPL25054

16 Samples

Download data: TXT, XLS

(Submitter supplied) DNA amplified from Bacillus subtilis strain JH642 chromosomal DNA using ORFmer primer set from Sigmagenosys as well as additional oligos. PCR products were purified, lyophilized, and resuspended in 50% DMSO. Products were spotted on to Corning GAPS II slides.

Organism:

Bacillus subtilis

1 Series

12 Samples

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(Submitter supplied) Please read Britton et al. Genome-wide analysis of the stationary-phase sigma factor (sigma-H) regulon of Bacillus subtilis. J Bacteriol. 2002 Sep;184(17):4881-90.

Organism:

Bacillus subtilis

1 Series

24 Samples

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(Submitter supplied) The gene expression of Bacillus subtilis 168 showed 3 major patterns including early expression, transition expression and late expression We monitored Bacillus subtilis gene expression by using microarray at differernt time points

Organism:

Bacillus subtilis; Bacillus subtilis subsp. subtilis str. 168

Type:

Expression profiling by array

Platform:

GPL343

7 Samples

Download data: CEL, CHP

(Submitter supplied) Transcriptional profile of the rocG gudB double null mutant during log growth phase in LB medium.

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL7420

2 Samples

Download data: GPR

(Submitter supplied) The numerous sigma factors present in Mycobacterium tuberculosis (MTB) are indicative of adaptability to different environmental conditions. In this report we describe the sigma factor B (sigB) regulon and the phenotypes of a MTB sigB mutant strain exposed to different stresses like SDS and Diamide. This experiment set compares expression profiles between H37Rv wild type and H37Rv sigB null mutant as well as under different stress conditions. more...

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by array

Platform:

GPL8523

36 Samples

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(Submitter supplied) We have determined the time-resolved transcriptome of the model gram-positive organism B. subtilis during growth on rich medium. DNA microarrays were used to monitor gene transcription in 10-minute intervals at 40 consecutive timepoints. From the growth curve and a PCA analysis of all gene expression levels, we identified 4 distinct growth phases: a lag phase, an exponential growth phase, and very clearly separated early and late stationary growth phases

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL6031

118 Samples

Download data: TXT