GEO DataSets

(Submitter supplied) Streptococcus gordonii, an important primary colonizer of dental plaque biofilm, specifically binds to salivary amylase via the surface-associated amylase-binding protein A (AbpA). We hypothesized that amylase binding to S. gordonii modulates expression of chromosomal genes, which could influence bacterial survival and persistence in the oral cavity. Gene expression profiling by microarray analysis was performed to detect differentially expressed genes in S. more...

Organism:

Streptococcus gordonii str. Challis

Type:

Expression profiling by array

Platform:

GPL14352

4 Samples

Download data: TXT

(Submitter supplied) Multiple levels of interkingdom signaling have been implicated in maintaining the ecological balance between Candida albicans and commensal streptococci to assure a state of oral health. To better understand the molecular mechanisms involved in the initial streptococcal response to the presence of C. albicans that can initiate oral surface colonization and biofilm formation, hypha-forming cells were incubated with Streptococcus gordonii cells for 30 minutes to assess the streptococcal transcriptome response. more...

Organism:

Streptococcus gordonii

Type:

Expression profiling by array

Platform:

GPL20180

2 Samples

Download data: TXT

(Submitter supplied) Our previous work has indicated that extracellular arginine concentrations may be an important stimulus for gene regulation in S gordonii. Here cells were cultured in a chemically defined medium (CDM) to mid exponential phase . Cultures were split and resuspended in either CDM or CDM lacking arginine. After 30 mins RNA was extracted and gene expression was monitored using an Agilent microarray.

Organism:

Streptococcus gordonii

Type:

Expression profiling by array

Platform:

GPL17786

8 Samples

Download data: TXT

(Submitter supplied) All organisms throughout the tree of life sense and respond to their surface environments. To discriminate from among mucosal surface environmental cues, we grew Streptococcus gordonii biofilms over night at 37C on surfaces coated with the salivary mucin MUC5B, or a low density protein fraction derived from human saliva.

Organism:

Streptococcus gordonii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20134

12 Samples

Download data: TXT

(Submitter supplied) Recent studies have shown that the transcriptional landscape of the pleiomorphic fungus Candida albicans is highly dependent upon growth conditions. Here using a dual RNA-seq approach we identified 299 C. albicans and 72 Streptococcus gordonii genes that were either up- or down-regulated specifically as a result of co-culturing these human oral cavity microorganisms. Seventy five C. albicans genes involved in responses to chemical stimuli, regulation, homeostasis, protein modification and cell cycle were statistically (P ≤0.05) upregulated, while 36 genes mainly involved in transport and translation were down-regulated. more...

Organism:

Candida albicans; Streptococcus gordonii

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20135 GPL19036 GPL20134

15 Samples

Download data: TXT

(Submitter supplied) Oral streptococci, including Streptococcus gordonii, and Actinomyces naeslundii, are consistently found to be the most abundant bacteria in the early stages of dental plaque accumulation. These organisms interact physically (coaggregate) in vitro and in vivo. We hypothesized that coaggregation between S. gordonii and A. naeslundii leads to changes in gene expression in the partner organisms. Furthermore, we predicted that coaggregation-induced changes in phenotype contribute to the success of streptococci and actinomyces in dental plaque. more...

Organism:

Streptococcus gordonii str. Challis

Type:

Expression profiling by array

Platform:

GPL5736

3 Samples

Download data: MEV

(Submitter supplied) Streptococcus gordonii is a primary colonizer of the multispecies biofilm on tooth surfaces forming dental plaque, and a potential agent of endocarditis. The recent completion of the genome sequence of the naturally competent strain Challis allowed the design of a spotted oligonucleotide microarray to examine a genome-wide response of this organism to environmental signals. Based on temporal responses to synthetic competence signaling peptide (CSP) as indicated by transformation frequencies, the S. more...

Organism:

Streptococcus gordonii str. Challis

Type:

Expression profiling by array

Platform:

GPL5736

6 Samples

Download data: PDF, XLS