GEO DataSets

(Submitter supplied) Gene expression profiles of human BM-MSC isolated form normal donor to elucidate potential molecular network for clinical application

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

4 Samples

Download data: CEL

(Submitter supplied) Expression of HIF-1a or Twist1 or Bmi1 in human hypopharyngeal cancer cell line FADU results in the drift of transcriptome profile from an epithelial cell-like signature to a mesenchymal stem cell-like signature.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

4 Samples

Download data: CEL, CHP

(Submitter supplied) Polycomb group (PcG) proteins are essential for accurate axial body patterning during embryonic development. PcG-mediated repression is conserved in metazoans and is targeted in Drosophila by Polycomb response elements (PREs). Targeting sequences in humans have not been described. While analyzing chromatin architecture in the context of human embryonic stem cell (hESC) differentiation, we discovered a 1.8kb region between HOXD11 and HOXD12 (D11.12) that is associated with PcG proteins, is nuclease hypersensitive, and shows alteration as hESCs differentiate. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL9673

10 Samples

Download data: TXT

(Submitter supplied) Duplication of chromosomal arm 20q occurs in prostate, cervical, colon, gastric, bladder, melanoma, pancreas and breast cancer, suggesting that 20q amplification may play a key causal role in tumorigenesis. According to an alternative view, chromosomal instabilities are mainly a common side effect of cancer progression. To test whether a specific genomic aberration might serve as a cancer initiating event, we established an in vitro system that models the evolutionary process of early stages of prostate tumor formation; normal prostate cells were immortalized and cultured for 650 days till several transformation hallmarks were observed. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4110

Platform:

GPL571

27 Samples

Download data: CEL, CHP

Temporal analysis of EP156T cells infected with a recombinant retrovirus encoding either p53R175H mutant (M cells), dominant-negative p53 peptide GSE56 (G cells) or control vector (C cells). Results provide insight into molecular mechanisms underlying early stages of transformation.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 7 genotype/variation, 3 infection, 27 other, 9 time sets

Platform:

GPL571

Series:

GSE23038

27 Samples

Download data: CEL, CHP

(Submitter supplied) Molecular mechanisms that regulate the generation of hematopoietic and endothelial cells from mesoderm are poorly understood. To define the underlying mechanisms, we compared gene expression profiles between embryonic stem (ES) cell-derived hemangioblasts (Blast-Colony-Forming Cells, BL-CFCs) and their differentiated progeny, Blast cells. Bioinformatic analysis indicated that BL-CFCs resembled other stem cell populations. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

4 Samples

Download data: CEL

(Submitter supplied) Direct contact with mesenchymal stromal impacts on migratory behavior and gene expression profile of CD133+ hematopoietic stem cells during ex-vivo expansion Objective: To investigate the impact of direct contact between mesenchymal stromal cells (MSCs) and CD133+ hematopoietic stem cells (HSCs) in terms of expansion potential differentiation, migratory capacity and gene expression profile. Methods: CD133+ purified HSCs were cultured for 7 days on subconfluent MSCs supplemented with growth factor containing medium. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

1 Sample

Download data: TXT

(Submitter supplied) MEF cells were sequentially infected with H-RasV12 and Twist (Twist1 or Twist2) expression retroviral constructs. Gene expression profiles of the resulting transformed cell lines were compared to the gene expression profile of primary MEF cells

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2897

6 Samples

Download data

(Submitter supplied) Lin-, CD38-, CD34+ hematopoietic stem cells. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL96 GPL97

6 Samples

Download data: CEL, EXP

(Submitter supplied) Using a TWIST1-inducible epithelial-to-mesenchymal transition (EMT) model in HMLE cells, miRNA changes were profiled at different time points during an active EMT.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL18795

12 Samples

Download data: TXT

(Submitter supplied) Epithelial-to-mesenchymal transition (EMT) is a dynamic process that relies on cellular plasticity; an EMT/MET axis is critical for metastatic colonization of carcinomas. Unlike epithelial programming, regulation of mesenchymal programming is not well understood in EMT. Here we describe the first microRNA that enhances exclusively mesenchymal programming. We demonstrate that microRNA-424 is up-regulated early during a TWIST1/SNAI1-induced EMT, and that it causes cells to express mesenchymal genes without affecting epithelial genes, resulting in a mixed/intermediate EMT. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

6 Samples

Download data: TXT

(Submitter supplied) GATA2 is essential for the endothelial-to-hematopoietic transition (EHT) and generation of hematopoietic stem cells (HSCs). It is poorly understood how GATA2 controls the development of human pluripotent stem cell (hPSC)-derived HSC-like cells. Here, using human embryonic stem cells (hESCs) in which GATA2 overexpression was induced by doxycycline (Dox), we elucidated the dual functions of GATA2 in definitive hematopoiesis before and after the emergence of CD34+CD45+CD90+CD38- HSC-like cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20795

9 Samples

Download data: CSV

(Submitter supplied) Analysis of BRMS1 KD-induced EMT in non-samll cell lung cancer at gene expression level. The hypothesis tested in the present study was that BRMS1 KD induces EMT through differential regulation of EMT genes and Twist1 KD restores the epithelial phenotype in cells with BRMS1 KD. Results provide important information of biological functions in lung cancer which BRMS1 KD involves in, such as EMT, signaling, biological adhesion, immune system process, response to stimulus, and so on.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

6 Samples

Download data: TXT

(Submitter supplied) A gene expression profiling suggested some gene candidates associated with colorectal cancer (CRC) metastasis, which were validated by qPCR investigation of the mRNA levels in clinical specimens

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1708

4 Samples

Download data: TXT

(Submitter supplied) The critical role of Bmi1 in promoting stem cell properties has been shown in different type of human cancers. Here, we established four stable clones to study Bmi-regulated miRNA expression patterns in head and neck caners.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platforms:

GPL8227 GPL10656

4 Samples

Download data: TXT

(Submitter supplied) To screen the microRNA regulated by Twist1 and Bmi1

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL10656

3 Samples

Download data: TXT

(Submitter supplied) Expression of let-7i results in transcriptome alteractions in head and neck cancer cell line, OECM1.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

2 Samples

Download data: CEL, CHP

(Submitter supplied) We generated GATA2/eGFP knockin human embryonic stem cells (H1-GATA2W/eGFP hESCs) and analyzed eGFP expression at different hematopoietic stages, paritcularly the hemogenic endothelium (HE) and hematopoietic progenitor cell (HPC) stage. Our results revealed that, at the HE stage, eGFP expression discriminates authentic HE from non-hematopoietic endothlial cells (EC) and, at the HPC stage, eGFP expression marks the HPC. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15520

4 Samples

Download data: TXT

(Submitter supplied) Bmi1 is a component of the Polycomb-repressive complexes (PRC) and essential for maintaining the pool of adult stem cells. PRC are key regulators for embryonic development by modifying chromatin architecture and maintaining gene repression. To assess the role of Bmi1 in pluripotent stem cells and upon exit from pluripotency during differentiation, we studied forced Bmi1 expression in mouse embryonic stem cells (ESC). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4211

Platform:

GPL1261

8 Samples

Download data: CEL

Analysis of Bmi1 transduced CCE embryonic stem cells (ESC) and CCE ESC differentiated by embryoid body assay. Bmi1 is essential for maintaining the pool of adult stem cells. Results provide insight into role of Bmi1 in ESC-derived hematopoietic cell transition from pluripotency to differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 5 genotype/variation sets

Platform:

GPL1261

Series:

GSE20958

8 Samples

Download data: CEL