GEO DataSets

(Submitter supplied) Fatty acid-amino acid conjugates (FACs) in the oral secretion (OS) of the Manduca sexta larvae are necessary and sufficient to elicit the herbivory-specific responses in Nicotiana attenuata, an annual wild tobacco species. We used SuperSAGE combined with 454 sequencing to quantify the early transcriptional changes elicited by the FAC N-linolenoyl-glutamic acid (18:3-Glu) in N. attenuata. The second fully expanded leaf of rosette stage plants were wounded by rolling a fabric pattern wheel three times on each side of the midvein and the wounds were immediately supplied with either 20 uL of 0.02% (v/v) Tween-20/water (solvent control) or 10 uL of synthetic N-linolenoyl-glutamic acid (18:3-Glu; 0.03 nmol/µL in 0.02% (v/v) Tween-20/water). more...

Organism:

Nicotiana attenuata

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9556

2 Samples

Download data: TXT

(Submitter supplied) Idenfication and characterization of oscillating transcripts after elicitation with oral secretions from the sepcialist herbivore, Manduca sexta larvae

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL13527

134 Samples

Download data: TXT

(Submitter supplied) Identification of transcripts that change expression in Nicotiana attenuata plants with reduced expression of lectin receptor kinase 1 after elicitation with oral secretions of Manduca sexta larvae.

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL13527

6 Samples

Download data: TXT

(Submitter supplied) Oviposition by lepidopteran herbivores on Nicotiana attenuata primes plant defence responses that are induced by the feeding larvae. While oviposition by both the generalist Spodoptera exigua and the specialist Manduca sexta primes the production of defensive phenylpropanoids, their larvae are differentially affected. We investigate here the impact of prior oviposition on the transcriptome and phytohormone levels of plants that were later attacked by larvae to find regulatory signals of this priming. more...

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL25245

27 Samples

Download data: TXT

(Submitter supplied) Identification of significantly altered transcripts in Nicotiana attenuata plants with reduced MYC2 expression after induction with oral secretions of Manduca sexta larvae.

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL13527

6 Samples

Download data: TXT

(Submitter supplied) Identification of transcripts that change expression in roots of Nicotiana attenuata plants with reduced expression of HSPRO and in association with Piriformospora indica.

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL13527

6 Samples

Download data: TXT

(Submitter supplied) DICER-like proteins produce small RNAs that silence genes involved in development and defenses against viruses and pathogens. Which DCLs participate in plant-herbivore interactions remains unstudied. We identified four distinct DCL genes and stably silenced their expression by RNAi in Nicotiana attenuata, a model system for the study of plant-herbivore interactions. Silencing DCL1 expression was lethal to the plants. more...

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL13527

9 Samples

Download data: TXT

(Submitter supplied) Summary: Multi-toxins Bt-crops carrying insecticidal toxins with similar host spectrum and different mode of action e.g. Cry and Vip, are expected to improve resistance management in target pests. Control failure has been informed for Cry toxins but not for Vip3A, of which no mechanism of resistance has yet been identified. Here we applied HT-SuperSAGE to analyze the transcriptome profiling in the midgut tissue of a tobacco budworm Heliothis virescens (F.) strain laboratory-selected for Vip3A resistance. more...

Organism:

Heliothis virescens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20832

2 Samples

Download data: TXT

(Submitter supplied) We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined “High-Throughput (HT-) SuperSAGE”. SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. In the present protocol, index (barcode) sequences are employed to discriminate tags from different samples. Such barcodes permit to enable researchers to analyze digital tags from many transcriptomes of many samples in a single sequencing run by simply pooling the libraries. more...

Organism:

Arabidopsis thaliana; Oryza sativa; Danio rerio

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL9316 GPL9319 GPL9302

31 Samples

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