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Links from GEO DataSets

Items: 20

1.

Genopal: a novel platform for focused microarray analysis

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Homo sapiens
Type:
Expression profiling by array
Platforms:
GPL4133 GPL9283
27 Samples
Download data: TXT
Series
Accession:
GSE18316
ID:
200018316
2.

Focused transcription profiling of peripheral blood mononuclear cells from vasculitis patients

(Submitter supplied) Peripheral blood mononuclear cells (PBMCs) from 13 vasculitis patients expressed varying gene profiles.
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL9283
14 Samples
Download data: TXT
Series
Accession:
GSE18247
ID:
200018247
3.

Comparative transcription profiling of peripheral blood mononuclear cells from vasculitis patients

(Submitter supplied) Peripheral blood mononuclear cells (PBMCs) from 13 vasculitis patients expressed varying gene profiles.
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL4133
13 Samples
Download data: TXT
Series
Accession:
GSE16945
ID:
200016945
4.

cRNA amplification methods enhance microarray identification of transcripts expressed in the nervous system

(Submitter supplied) Background: DNA microarrays provide a powerful method for global analysis of gene expression. The application of this technology to specific cell types and tissues, however, is typically limited by small amounts of available mRNA, thereby necessitating amplification. Here we compare microarray results obtained with two different methods of RNA amplification to profile gene expression in the C. elegans larval nervous system. more...
Organism:
Caenorhabditis elegans
Type:
Expression profiling by array
Platform:
GPL200
8 Samples
Download data: CEL, CHP
Series
Accession:
GSE9485
ID:
200009485
5.

Flexible multiplatform RNA profiling at the single cell level applied to enriched cancer initiating cells

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Homo sapiens
Type:
Expression profiling by array; Expression profiling by high throughput sequencing; Expression profiling by RT-PCR
Platforms:
GPL17989 GPL570 GPL16288
64 Samples
Download data: CEL
Series
Accession:
GSE52717
ID:
200052717
6.

Flexible multiplatform RNA profiling at the single cell level applied to enriched cancer initiating cells: RNA-Seq MCF7 and MCF10A single cell data

(Submitter supplied) Accurate profiling of RNA expression of single cells is a valuable approach for broadening our understanding of cancer biology and mechanisms of dissemination, but requires the development of reliable methods for their molecular characterization. Here we evaluate a single cell methodology which generates microgram amounts of cDNA suitable for next generation sequencing (RNA-Seq), high throughput RT-qPCR and Affymetrix array analysis. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL16288
10 Samples
Download data: XLSX
Series
Accession:
GSE52716
ID:
200052716
7.

Flexible multiplatform RNA profiling at the single cell level applied to enriched cancer initiating cells: RNA-Seq CIC data

(Submitter supplied) Accurate profiling of RNA expression of single cells is a valuable approach for broadening our understanding of cancer biology and mechanisms of dissemination, but requires the development of reliable methods for their molecular characterization. Here we evaluate a single cell methodology which generates microgram amounts of cDNA suitable for next generation sequencing (RNA-Seq), high throughput RT-qPCR and Affymetrix array analysis. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL16288
12 Samples
Download data: XLSX
Series
Accession:
GSE52715
ID:
200052715
8.

Flexible multiplatform RNA profiling at the single cell level applied to enriched cancer initiating cells: RT-PCR

(Submitter supplied) Accurate profiling of RNA expression of single cells is a valuable approach for broadening our understanding of cancer biology and mechanisms of dissemination, but requires the development of reliable methods for their molecular characterization. Here we evaluate a single cell methodology which generates microgram amounts of cDNA suitable for next generation sequencing (RNA-Seq), high throughput RT-qPCR and Affymetrix array analysis. more...
Organism:
Homo sapiens
Type:
Expression profiling by RT-PCR
Platform:
GPL17989
22 Samples
Download data: TXT
Series
Accession:
GSE52714
ID:
200052714
9.

Flexible multiplatform RNA profiling at the single cell level applied to enriched cancer initiating cells: Affymetrix array data

(Submitter supplied) Accurate profiling of RNA expression of single cells is a valuable approach for broadening our understanding of cancer biology and mechanisms of dissemination, but requires the development of reliable methods for their molecular characterization. Here we evaluate a single cell methodology which generates microgram amounts of cDNA suitable for next generation sequencing (RNA-Seq), high throughput RT-qPCR and Affymetrix array analysis. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL570
20 Samples
Download data: CEL
Series
Accession:
GSE52712
ID:
200052712
10.

Spotted long oligonucleotide arrays

(Submitter supplied) Both spotted long oligonucleotide arrays and Affymetrix GeneChips were used to measure differential gene expression in two RNA samples (K562 erythroleukemia RNA and the Stratagene Universal Human Reference RNA). For Affymetrix technology, the two RNAs were analyzed separately. There are two replicates for K562 RNA (GSM4843 and GSM4844) and three for the Stratagene Universal reference (GSM4845-GSM4847). more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Datasets:
GDS222 GDS223
Platforms:
GPL273 GPL8300 GPL274
23 Samples
Download data: CEL
Series
Accession:
GSE344
ID:
200000344
11.
Full record GDS223

Microarray technology comparison (HG-U95A)

Part of a comparison of spotted long oligonucleotide arrays with in situ synthesized 25-mer arrays. Examination of amplified and unamplified K562 erythroleukemia RNA and Stratagene Universal Human Reference RNA.
Organism:
Homo sapiens
Type:
Expression profiling by array, transformed count, 2 cell type sets
Platform:
GPL8300
Series:
GSE344
5 Samples
Download data: CEL
12.
Full record GDS222

Microarray technology comparison (3OPHs version 1)

Part of a comparison of spotted long oligonucleotide arrays with in situ synthesized 25-mer arrays. Examination of amplified and unamplified K562 erythroleukemia RNA and Stratagene Universal Human Reference RNA.
Organism:
Homo sapiens
Type:
Expression profiling by array, log ratio, 3 protocol sets
Platform:
GPL273
Series:
GSE344
14 Samples
Download data
13.

Evaluation of the performance of the Arabidopsis Pathoarray 464_001: an example of detailed analysis of mutants

(Submitter supplied) The Arabidopsis Pathoarray 464_001 (GPL3638) was used to compare response of wild-type, rps2-101C (Bent et al., 1994; Mindorinos et al., 1994) and ndr1-1 (Century et al., 1995) to Pseudomonas syringae strain expressing avrRpt2. The two mutants are compromised in RPS2-mediated resistance but distinct difference between them has not been described. The results suggested that ndr1-1 affects a defense signaling pathway(s) in addition to the RPS2-dependent pathway, and indicate that the microarray is a powerful tool for systems analysis of the Arabidopsis disease signaling network. more...
Organism:
Arabidopsis thaliana
Type:
Expression profiling by array
Platform:
GPL3638
8 Samples
Download data: GPR
Series
Accession:
GSE5308
ID:
200005308
14.

Evaluation of performance of Arabidopsis Pathoarray 464_001: comparison with ATH1

(Submitter supplied) To evaluate technical reproducibility of Arabidopsis Pathoarray 464_001 (GPL3638), two slides were hybridized with labeled amplified RNA prepared from identical RNA. The RNA was prepared from Pseudomonas syringae ES4326- and 5mM MgSO4- injected samples (Psm 24h set C and mock 24h set C. See also GSE4429, GSM99793 and GSM99794). Keywords: Evaluation of a custom microarray performance
Organism:
Arabidopsis thaliana
Type:
Expression profiling by array
Platform:
GPL3638
4 Samples
Download data: GPR
Series
Accession:
GSE4632
ID:
200004632
15.

Analysis of Arabidopsis thaliana gene expression in response to the bacterial pathogen Pseudomonas syringae ES4326

(Submitter supplied) In order to protect themselves from pathogens, plants activate a battery of defense pathways, many of which involve changes in gene expression. We are interested in identifying plant genes that are differentially expressed in response to pathogen exposure, with the ultimate goal of studying the roles of these genes in plant defense. Keywords: disease response
Organism:
Arabidopsis thaliana
Type:
Expression profiling by array
Platform:
GPL198
2 Samples
Download data
Series
Accession:
GSE4429
ID:
200004429
16.

Gene Expression Profiling of Whole Blood: Comparison of target preparation methods

(Submitter supplied) Peripheral blood is an accessible and informative source of transcriptomal information for many human disease and pharmacogenomic studies. While there can be significant advantages to analyzing RNA isolated from whole blood, particularly in clinical studies, the preparation of samples for microarray analysis is complicated by the need to minimize artifacts associated with highly abundant globin RNA transcripts. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL201
24 Samples
Download data: CEL, CHP
Series
Accession:
GSE13292
ID:
200013292
17.

Universal Reference RNA as a standard for microarray experiments

(Submitter supplied) Abstract: BACKGROUND: Obtaining reliable and reproducible two-color microarray gene expression data is critically important for understanding the biological significance of perturbations made on a cellular system. Microarray design, RNA preparation and labeling, hybridization conditions and data acquisition and analysis are variables difficult to simultaneously control. A useful tool for monitoring and controlling intra- and inter-experimental variation is Universal Reference RNA (URR), developed with the goal of providing hybridization signal at each microarray probe location (spot). more...
Organism:
Homo sapiens; Mus musculus
Type:
Expression profiling by array
6 related Platforms
31 Samples
Download data
Series
Accession:
GSE4389
ID:
200004389
18.

Contribution of unique genes by individual cell lines to Universal Mouse Reference RNA

(Submitter supplied) RNA from 11 individual mouse cell lines were reverse-transcribed to cDNA, labeled with Cy5 and cohybridized with Cy3-labeled UMRR onto 7,500-spot mouse oligo microarrays (UNC). The data was analyzed using GeneTraffic software. 300-1000 spots out of 8,000 were flagged on each microarray and excluded from further analysis. Spots with hybridization signals in Cy5 channel higher than 1000 and with Cy5/Cy3 ratio greater than 2 were collected and the number of spots with these characteristics on only one microarray was determined. more...
Organism:
Mus musculus
Type:
Expression profiling by array
Platform:
GPL3304
14 Samples
Download data
Series
Accession:
GSE3974
ID:
200003974
19.

Microarray Coverage of Universal Human Reference RNA on microarrays with yeast controls

(Submitter supplied) Microarray coverage of UHRR was determined by counting the number of spots with intensities exceeding the 1% and 5% false positive rates defined using the yeast negative control spots. Seven microarrays from different print runs were used. In four experiments, the UHRR was labeled with Cy5 and in three experiments with Cy3. Labeled UHRR was co-hybridized with Common Reference RNA (CRF) developed at Stanford University (8) labeled with the alternate dye. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
4 related Platforms
7 Samples
Download data
Series
Accession:
GSE3973
ID:
200003973
20.

Contribution of unique genes by individual cell lines to Universal Human Reference RNA

(Submitter supplied) Total RNA isolated from 10 individual human cell lines were reverse-transcribed to cDNA, labeled with Cy5 and co-hybridized with Cy3-labeled UHRR onto 43,000-spot cDNA microarrays (Stanford University). The data was analyzed using GeneTraffic software. Approximately 6000-8000 spots out of 43,000 (14-18 %) were flagged on each microarray and excluded from further analysis. Spots with hybridization signals in Cy5 channel higher than 1000 and with Cy5/Cy3 ratio greater than 2 were collected and the number of spots with these characteristics in only one cell line was determined. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Dataset:
GDS1835
Platform:
GPL3305
10 Samples
Download data
Series
Accession:
GSE3972
ID:
200003972
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