GEO DataSets

(Submitter supplied) Insect hemocytes mediate important cellular immune responses including phagocytosis and encapsulation, and also secrete immune factors such as opsonins, melanization factors, and antimicrobial peptides. In Anopheles, they contribute to the defense against malaria parasite invasion during the early sporogonic cycle. We used microarrays to identify transcripts that are specific or enriched in circulating hemocytes compared to either neuronal or to the rest of the body.

Organism:

Anopheles gambiae; Plasmodium falciparum

Type:

Expression profiling by array

Platform:

GPL1321

16 Samples

Download data: CEL, CHP

(Submitter supplied) Insect hemocytes mediate important cellular immune responses including phagocytosis and encapsulation, and also secrete immune factors such as opsonins, melanization factors, and antimicrobial peptides. In Anopheles, they contribute to the defense against malaria parasite invasion during the early sporogonic cycle. We used microarrays to identify if and to what degree circulating hemocytes have altered global expression profiles after infection with the rodent malaria parasite, Plasmodium berghei

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

6 Samples

Download data: CEL, CHP

(Submitter supplied) Background: The mosquito Anopheles gambiae is a major vector of human malaria. Increasing evidence indicates that blood cells (hemocytes) comprise an essential arm of the mosquito innate immune response against both bacteria and malaria parasites. To further characterize the role of hemocytes in mosquito immunity, we undertook the first genome-wide transcriptomic analyses of adult female An. gambiae hemocytes following infection by two species of bacteria and a malaria parasite. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by array

Platforms:

GPL8551 GPL8550 GPL8552

11 Samples

Download data

(Submitter supplied) We present a microarray study of hemocyte-enriched transcriptome in Aedes aegypti. We directed our efforts to critically assess the transcriptomic features distinctive of hemocytes, and discussed our findings in relation to infection-responsive genes and immune-relate gene families. Specifically, our analysis examined how tissue-enriched expression patterns change with the immune status of the host, and resolved patterns of transcriptional change unique to hemocytes from those that are likely shared by other immune responsive tissues.

Organism:

Aedes aegypti

Type:

Expression profiling by array

Platform:

GPL4877

40 Samples

Download data: GPR

(Submitter supplied) To test the contributions of phagocytic immune cells to mosquito immunity, we depleted mosquito phagocytes using clodronate liposomes and then challenged mosquitoes with malaria parasites. Observing a large increase in infection intensity, the purpose of the RNA-seq experiment is to determine their effects of phagocyte depletion on mosquito immunity.

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25232

8 Samples

Download data: TXT

(Submitter supplied) Anopheles gambiae, the primary African malarial mosquito, exhibits numerous behaviors that are under diel and circadian control, including locomotor activity, swarming, mating, host seeking, eclosion, egg laying and sugar feeding. However, little has been performed to elucidate the molecular basis for these daily rhythms. To study how gene expression is globally regulated by diel and circadian mechanisms, we have undertaken a DNA microarray analysis of A. more...

Organism:

Anopheles gambiae; Plasmodium falciparum

Type:

Expression profiling by array

Dataset:

GDS4261

Platform:

GPL1321

104 Samples

Download data: CEL

Analysis of heads and bodies of mated, non-blood-fed, adult females maintained under a 12hr:12hr light:dark (LD) cycle or constant dark (DD) conditions; samples were collected every 4hr for 48 hr. Results provide insight into molecular mechanisms under circadian and LD cycle control.

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array, transformed count, 2 growth protocol, 26 time, 2 tissue sets

Platform:

GPL1321

Series:

GSE22585

104 Samples

Download data: CEL

(Submitter supplied) Malaria is as one of the most debilitating mosquito-borne global health burdens. While much of the malaria and mosquito-borne disease attention have focused on Africa, South East Asia accounts for a sizable portion of the malaria global burden. Moreover, about 50% of the Asian malaria incidence and deaths have been from India. The completion of genome sequence of Anopheles stephensi, a major malaria vector in Asia, offers new opportunities for global health innovation, not to mention for progress in deciphering the vectorial ability of this mosquito species at a molecular level. more...

Organism:

Anopheles stephensi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23543

4 Samples

Download data: GTF, TXT

(Submitter supplied) Wolbachia, an endosymbiotic bacterium, is being investigated as a vector control agent in several insect species. Along with the well known classical reproductive parasitism Wolbachia employs against its host to spread within the population, it is emerging that the bacteria can protect the host against pathogens and reduced pathogen transmission. Anopheles mosquitoes, which transmit malaria, have never been found to harbour Wolbachia in nature, and despite numerous transinfection attempts, no stable line has been developed. more...

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

9 Samples

Download data: CEL

(Submitter supplied) We provide a broad overview of sequence diversity in An. gambiae mature microRNAs, including annotation of novel microRNAs identified in this study.

Organism:

Anopheles gambiae

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL15693 GPL17644

8 Samples

Download data: TXT

(Submitter supplied) Anopheles gambiae mosquitoes play an important role in malaria transmission. In sub-Saharan Africa, the dry season can last several months. The mechanisms for mosquito population to survive through the dry season are poorly understood. One possible mechanism is that adults increase their desiccation tolerance over the dry season. Genetic analyses have shown that inversions 2La, 2Rb, 2Rc, 2Rd and 2Ru are associated with aridity resistance, however little is known about the transcriptional response of genes in response to desiccation. more...

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Dataset:

GDS4552

Platform:

GPL1321

19 Samples

Download data: CEL

Analysis of adult females exposed to 30% (desiccated) relative humidity for up to 36 hrs. The An. gambiae malaria vector populations are observed year round but their densities are low during the dry season. Results provide insight into molecular basis of the malaria vector's desiccation response.

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array, count, 3 stress, 3 time sets

Platform:

GPL1321

Series:

GSE25433

19 Samples

Download data: CEL

(Submitter supplied) Background. The mosquito, Anopheles gambiae, is the primary vector of human malaria, a disease responsible for millions of deaths each year. To improve strategies for controlling transmission of the causative parasite, Plasmodium falciparum, we require a thorough understanding of the developmental mechanisms, physiological processes and evolutionary pressures affecting life-history traits in the mosquito. more...

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

60 Samples

Download data: CEL

(Submitter supplied) We conducted a genome-wide survey of genes in Ae. aegypti females that are transcriptionally responsive upon challenge with dengue virus (serotype-2). The array was designed with 60-mer oligos specific to 16,092 gene transcripts of gene build AaegL1.1 (www. vectorbase.org). The hybridizations were performed at NimbleGen. We provided total RNA purified from the infected and control samples to NimbleGen. more...

Organism:

Aedes aegypti

Type:

Expression profiling by array

Platform:

GPL8705

15 Samples

Download data: PAIR

(Submitter supplied) Malaria is caused by Plasmodium parasites, which are transmitted via the bites of infected Anopheline mosquitoes. Midgut invasion is a major bottleneck for Plasmodium development inside the mosquito vectors as a rapidly responding immune system recognizes ookinetes and recruits killing factors from the midgut and surrounding tissues, dramatically reducing the population of invading ookinetes before they can successfully traverse the midgut epithelium. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15693

18 Samples

Download data: TXT

(Submitter supplied) Drosophila hemocytes are the primary defense system required for the animals to thrive and overcome harmful threats and are often compared to the innate immune system of vertebrates. However, their homology has been described using only a limited number of genes and a systematic analysis using transcriptomic data would provide novel insights about the evolution of immune systems. In this study, we performed cross-species comparative analysis using single-cell RNA-sequencing data of Drosophila hemocytes, zebrafish, mouse, and human immune cells in both supervised and unsupervised manner. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17275

3 Samples

Download data: MTX, RDS, TSV, TXT