Similar studies for GEO DataSets (Select 200017733) - GEO DataSets

Links from GEO DataSets

Items: 20

Select item 2000177331.

Delineation of EZH2 oncogenic functions in hepatocellular carcinoma

(Submitter supplied) The goal of this study was to delineate the important EZH2 direct target genes that mediate the oncogenic properties of EZH2 in HCC. The EZH2 direct target genes in two HCC cell lines were identified by chromatin immunoprecipitation microarray (ChIP-chip) analysis and later confirmed by independent ChIP-PCR. The functions of the target genes were further examined.

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by genome tiling array

Platforms:: GPL4125 GPL4124
8 Samples

Download data: TXT

Series

Accession:: GSE17733

ID:: 200017733

Select item 2000688632.

Expression analysis of sk-hep-1(sk) cell and sk-hep-1-sh EZH2(sks) cell

(Submitter supplied) Investigation of EZH2 knocking down on whole genome gene expression level changes in sk-hep-1 compared to sk-hep-1-sh EZH2.

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL18943
2 Samples

Download data: CALLS, PAIR

Series

Accession:: GSE68863

ID:: 200068863

Select item 2000687583.

Genome-wide maps of H3K27me3 modification sites in sk-hep-1,sk-hep-1-shEZH2 and LO2 cells.

(Submitter supplied) We report the application of chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) for high-throughput profiling of H3K27me3 modifications in sk-hep-1(sk), sk-hep-1-shEZH2 (sks) and LO2 cells. Following standard ChIP procedure, 10 ng of each DNA sample was used for Illumina sequencing. Statistically significant ChIP-enriched regions (peaks) were identified by sk vs sks (p-value threshold of 10-2) or sk vs LO2 (p-value threshold of 10-3). more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL11154
3 Samples

Download data: BED, WIG, XLS

Series

Accession:: GSE68758

ID:: 200068758

PubMed Similar studies SRA Run Selector

Select item 2000035364.

Hepatoma cell lines vs. Universal control 3

(Submitter supplied) Gene expression profiles in hepatoma cells Keywords: Cell type comparison

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL2913
36 Samples

Download data

Series

Accession:: GSE3536

ID:: 200003536

Select item 2000035115.

Hepatoma cell lines vs. Universal control 2

(Submitter supplied) Gene expression profiles in hepatoma cells Keywords: Cell type comparison

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL2912
18 Samples

Download data

Series

Accession:: GSE3511

ID:: 200003511

Select item 2000034446.

Hepatoma cell lines vs. Universal control

(Submitter supplied) Gene expression profiles in hepatoma cells Keywords: Cell type comparison

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL2911
18 Samples

Download data

Series

Accession:: GSE3444

ID:: 200003444

Select item 2000541087.

A cDNA microarray analysis was performed on shRNA KDs of EZH2, SUZ12, BMI1, or CBX8 HepG2 cells.

(Submitter supplied) Polycomb group (PcG) proteins including EZH2, SUZ12 ,BMI1,CBX8 and so on, which specifically catalyze trimethylation of histone 3 lysine 27 (H3K27me3), and methylated H3K27 can be recognized by other specific binding proteins to compress chromatin structure, leading to the transcriptional repression of the target genes. To explore a potential functional implication of PcG components in HCC, we stably transfected HepG2 cells with either vectors or constructs expressing shRNA that specifically targets EZH2, SUZ12, BMI1, or CBX8. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL10332
5 Samples

Download data: TXT

Series

Accession:: GSE54108

ID:: 200054108

Select item 2000523008.

Chip-chip from HepG2 cells with EZH2, SUZ12 and H3K27me3

(Submitter supplied) Polycomb group (PcG) proteins including EZH2, SUZ12 and so on, which specifically catalyze trimethylation of histone 3 lysine 27 (H3K27me3), and methylated H3K27 can be recognized by other specific binding proteins to compress chromatin structure, leading to the transcriptional repression of the target genes. To completely understand the epigenetic profile and molecular network of PcG in HCC, we performed ChIP-on-chip screens with EZH2, SUZ12 and H3K27me3 antibodies in HepG2 cells.

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by genome tiling array

Platform:: GPL9448
3 Samples

Download data: GFF, JPG, PAIR, XLSX

Series

Accession:: GSE52300

ID:: 200052300

Select item 2000119569.

Comparison of TCF hyper-activated and control Huh7 cells

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Expression profiling by array; Expression profiling by SAGE

Platforms:: GPL4 GPL570
8 Samples

Download data: CEL, TAR

Series

Accession:: GSE11956

ID:: 200011956

Select item 20010106110.

Oncogenic Serine-Threonine Kinase Receptor Associated Protein Supports Hepatocellular Carcinoma Cell Growth by Enhancing Wnt/β-catenin Signaling

(Submitter supplied) Serine-threonine kinase receptor-associated protein (STRAP) is upregulated in breast, colorectal and lung cancers, promoting their growth. We identify the upregulation of STRAP in hepatocellular carcinomas. Elevated STRAP endows tumor cells with growth advantage by reprograming a variety of metabolic processes and signaling pathways critical for hepatocellular carcinoma progression. Especially, enhanced Wnt/β-catenin signaling is likely to be a major effector of its tumor-promoting role.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL11154
12 Samples

Download data: XLSX

Series

Accession:: GSE101061

ID:: 200101061

PubMed Similar studies SRA Run Selector

Select item 20002005411.

H3K27 Methyltransferase PRC2 Represses Wnt Genes to Facilitate Adipogenesis

(Submitter supplied) The Wnt/b-catenin signaling inhibits adipogenesis. Genome-wide profiling studies have revealed the enrichment of histone H3K27 methyltransferase PRC2 on Wnt genes. However, the functional significance of such a direct link between the two types of developmental regulators in mammalian cells, and the role of PRC2 in adipogenesis, remain unclear. Here we show PRC2 and its H3K27 methyltransferase activity are required for adipogenesis. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Dataset:: GDS3765
Platform:: GPL1261
4 Samples

Download data: CEL

Series

Accession:: GSE20054

ID:: 200020054

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 376512.

Histone H3K27 methyltransferase Ezh2 deletion effect on primary preadipocyte cultures

Analysis of preadipocytes deficient in Ezh2, the enzymatic subunit of H3K27 methyltransferase PRC2. Deletion of Ezh2 eliminates H3K27me3 on Wnt promoters leading to activation of Wnt/b-catenin signaling and inhibition of adipogenesis. Results provide insight into the role of PRC2 in adipogenesis.

Organism:: Mus musculus

Type:: Expression profiling by array, count, 2 genotype/variation sets

Platform:: GPL1261
Series:: GSE20054
4 Samples

Download data: CEL

DataSet

Accession:: GDS3765

ID:: 3765

PubMed Full text in PMC Similar studies GEO Profiles Analyze DataSet

Select item 20004161013.

mRNA expression after siRNA-mediated knock down of Enhancer of zeste homolog 2 (Ezh2) in human umbilical vein endothelial cells

(Submitter supplied) mRNA expression after Ezh2 knock down was analyzed to identify genes regulated by Ezh2.

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL6480
3 Samples

Download data: TXT

Series

Accession:: GSE41610

ID:: 200041610

Select item 20009687214.

Supraorbital cranial mesenchyme with ectoderm upon the deletion of β-catenin

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:: GPL17021 GPL19057
10 Samples

Download data: BW

Series

Accession:: GSE96872

ID:: 200096872

PubMed Full text in PMC Similar studies

Select item 20009687115.

Gene expression changes in the supraorbital cranial mesenchyme after beta-catenin deletion in vivo

(Submitter supplied) Goal of this study was to examine gene expression changes upon conditional deletion of beta-catenin at e13.5 cranial mesenchyme.

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17021
7 Samples

Download data: TXT, XLSX

Series

Accession:: GSE96871

ID:: 200096871

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20009660416.

H3K27me3 enrichment in the supraorbital cranial mesenchyme with ectoderm upon the deletion of β-catenin

(Submitter supplied) H3K27me3 enrichment in the supraorbital cranial mesenchyme with ectoderm upon the deletion of β-catenin

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL19057
3 Samples

Download data: BW

Series

Accession:: GSE96604

ID:: 200096604

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20007594417.

Gene expression changes in dorsal dermal fibroblasts after beta-catenin deletion in vivo

(Submitter supplied) Goal of this study was to examine gene expression changes upon conditional deletion of beta-catenin at E10.5 in dermal fibroblasts. Method: Skin tissues were treated in 0/25% trypsin for 15 min at 37 degrees C to obtain single cells for FACS sorting and collected in RNA later. RNA was extracted with Karcturus pico pure kit. The RT and amplification was done using Nugen's ovation RNA-seq system V2. Libraries for sequencing were prepared with Illumina TruSeq kit. Transcriptome of FACS sorted E13.5 Engrailed1; RRYFP lineage-marked dorsal dermal fibroblasts was generated next-gen sequencing, in triplicate, using Illumina HiSeq machine. The sequence reads that passed quality filters were analyzed by TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays Results: We mapped about 33-49 million sequence reads per sample and obtained 76-79% of uniquely mapped percentage. We assembled the reads to the mouse ref genome (build mm10).

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17021
5 Samples

Download data: XLSX

Series

Accession:: GSE75944

ID:: 200075944

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20020256118.

DNMT and EZH2 inhibitors synergize for gene activation in hepatocellular carcinoma cells.

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Methylation profiling by genome tiling array; Expression profiling by high throughput sequencing

Platforms:: GPL21145 GPL18573
48 Samples

Download data: IDAT

Series

Accession:: GSE202561

ID:: 200202561

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20020256019.

DNMT and EZH2 inhibitors synergize for gene activation in hepatocellular carcinoma cells [RNA-seq]

(Submitter supplied) Hepatocellular carcinoma (HCC) is the most common primary liver malignancy with cases increasing dramatically in the USA. Developing more effective targeted therapies for HCC is an urgent need. The FDA-approved DNA methyltransferase inhibitors (DNMTis) 5-azacytidine (5-aza-CR, Vidaza) and 5-aza-2’-deoxycytidine (5-aza-CdR, Decitabine) represented the first clinical breakthrough to target aberrant cancer epigenomes. more...