GEO DataSets

(Submitter supplied) Neutrophil lysis after phagocytosis is a process potentially important in the pathogenesis of community-associated methicillin-resistant S. aureus (CA-MRSA) infection. The mechanism for this process is not currently known. Therefore, to better understand CA-MRSA virulence we used human oligonucleotide microarrays to investigate the mechanism underlying enhanced PMN lysis that occurs after phagocytosis of CA-MRSA. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

113 Samples

Download data: CEL, CHP

(Submitter supplied) To measure S. aureus gene expression during growth phase and/or S. aureus–PMN interaction. Keywords: other

Organism:

Streptococcus pyogenes; Coxiella burnetii; Staphylococcus aureus; Borreliella burgdorferi; Yersinia pestis; Chlamydia trachomatis

Type:

Expression profiling by array

Platform:

GPL2129

159 Samples

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(Submitter supplied) Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are often caused by strains encoding Panton-Valentine leukocidin (PVL). PVL can cause lysis of polymorphonuclear leukocytes (PMNs) and other myeloid cells in vitro, a function considered widely as the primary means by which PVL might contribute to disease. However, at sublytic concentrations PVL can function as a PMN agonist. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

30 Samples

Download data: CEL, CHP

(Submitter supplied) Staphylococcus aureus strain MW2 was exposed to the following neutrophil microbicides, hydrogen peroxide (H2O2), hypochlorous acid (HOCl) and azurophilic granule proteins. At the indicated time points, bacterial cultures were centrifuged and bacteria were lysed with RLT buffer (Qiagen) using a FastPrep system. Purification of MW2 RNA and subsequent preparation of labeled cDNA target was performed as described in Methods. more...

Organism:

Staphylococcus aureus; Chlamydia pneumoniae AR39; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia caviae GPIC; Staphylococcus haemolyticus JCSC1435; Staphylococcus aureus subsp. aureus MW2; Granulibacter bethesdensis; Coxiella burnetii; Staphylococcus epidermidis ATCC 12228; Chlamydia trachomatis D/UW-3/CX; Rickettsia rickettsii; Chlamydia muridarum; Staphylococcus epidermidis RP62A

Type:

Expression profiling by array

Platform:

GPL4692

48 Samples

Download data: CEL

(Submitter supplied) Polymorphonuclear leukocytes (PMNs) were obtained from healthy individuals in accordance with protocols approved by the Institutional Review Board for Human Subjects at the University of Minnesota and the National Institute of Allergy and Infectious Diseases. PMNs (107) were combined on ice with live S. aureus (108) or with live or heat-killed A. phagocytophilum (bacteria isolated from 5x106 infected HL60 cells for a ratio of 1 infected HL60 cell: 2 PMNs, ~ 5-20 A. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

67 Samples

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(Submitter supplied) Approximatively 75% of the genome of S. aureus (“core” genome) are highly conserved between strains, whereas the remaining 25% (“accessory” genome) are composed of variable regions that are mostly composed of mobile genetic elements (MGE), containing virulence and resistance genes. We have developed a composite DNA-microarray (StaphVar Array) which selectively targets 403 genes located on the accessory or core variable genome. more...

Organism:

Staphylococcus aureus

Type:

Genome variation profiling by array

Platform:

GPL7684

26 Samples

Download data: GPR

(Submitter supplied) Staphylococcus aureus is a leading cause of bloodstream infections worldwide. In the United States, many of these infections are caused by a strain known as USA300. Although progress has been made, our understanding of the S. aureus molecules that promote bacteremia and survival in human blood is incomplete. To that end, we analyzed the USA300 transcriptome during culture in human blood, human serum, and trypticase soy broth (TSB), a standard laboratory culture media. more...

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by array

Platform:

GPL8069

80 Samples

Download data: CEL, CHP

(Submitter supplied) Methicillin-resistant Staphylococcus aureus (MRSA) is problematic both in hospitals and the community. Currently, we have limited understanding of mechanisms of innate immune evasion used by S. aureus. To that end, we created an isogenic deletion mutant in strain MW2 (USA400) of the saeR/S two-component gene regulatory system and studied its role in mouse models of pathogenesis and during human neutrophil interaction. more...

Organism:

Chlamydia pneumoniae AR39; Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus subsp. aureus MW2; Granulibacter bethesdensis; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia trachomatis D/UW-3/CX; Coxiella burnetii; Rickettsia rickettsii; Staphylococcus aureus; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia caviae GPIC; Staphylococcus haemolyticus JCSC1435

Type:

Expression profiling by array

Platform:

GPL4692

12 Samples

Download data: CEL, CHP

(Submitter supplied) It is becoming increasingly apparent that Staphylococcus aureus are able to survive engulfment by macrophages, and that the intracellular environment of these cells, which is essential to innate host defenses against invading microorganisms, may in fact provide a refuge for staphylococcal survival and dissemination. Based on this, we postulated that S. aureus might induce cytoprotective mechanisms by changing gene expression profiles inside macrophages similar to obligate intracellular pathogens, such as Mycobacterium tuberculosis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4931

Platform:

GPL570

30 Samples

Download data: CEL, CHP

Analysis of monocyte-derived macrophages that phagocytosed S. aureus in vitro. Monocytes analyzed for up to 48 hours after phagocytosis. Results provide insight into the molecular mechanisms employed by S. aureus to prolong their intracellular survival.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 infection, 3 time sets

Platform:

GPL570

Series:

GSE13670

30 Samples

Download data: CEL, CHP

(Submitter supplied) Necrosis is commonly found in various solid tumors and predicts worse outcome. Chronic ischemia can initiate tumor necrosis, but how the damaged tissue further expands is unclear. Previous studies found that neutrophils associate with necrosis and could contribute to necrosis development in glioblastoma (GBM) by transferring myeloperoxidase (MPO)-containing granules into tumor cells and inducing tumor cell ferroptosis. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

4 Samples

Download data: TXT