GEO DataSets

(Submitter supplied) Oral streptococci, including Streptococcus gordonii, and Actinomyces naeslundii, are consistently found to be the most abundant bacteria in the early stages of dental plaque accumulation. These organisms interact physically (coaggregate) in vitro and in vivo. We hypothesized that coaggregation between S. gordonii and A. naeslundii leads to changes in gene expression in the partner organisms. Furthermore, we predicted that coaggregation-induced changes in phenotype contribute to the success of streptococci and actinomyces in dental plaque. more...

Organism:

Streptococcus gordonii str. Challis

Type:

Expression profiling by array

Platform:

GPL5736

3 Samples

Download data: MEV

(Submitter supplied) Our previous work has indicated that extracellular arginine concentrations may be an important stimulus for gene regulation in S gordonii. Here cells were cultured in a chemically defined medium (CDM) to mid exponential phase . Cultures were split and resuspended in either CDM or CDM lacking arginine. After 30 mins RNA was extracted and gene expression was monitored using an Agilent microarray.

Organism:

Streptococcus gordonii

Type:

Expression profiling by array

Platform:

GPL17786

8 Samples

Download data: TXT

(Submitter supplied) Recent studies have shown that the transcriptional landscape of the pleiomorphic fungus Candida albicans is highly dependent upon growth conditions. Here using a dual RNA-seq approach we identified 299 C. albicans and 72 Streptococcus gordonii genes that were either up- or down-regulated specifically as a result of co-culturing these human oral cavity microorganisms. Seventy five C. albicans genes involved in responses to chemical stimuli, regulation, homeostasis, protein modification and cell cycle were statistically (P ≤0.05) upregulated, while 36 genes mainly involved in transport and translation were down-regulated. more...

Organism:

Streptococcus gordonii; Candida albicans

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20135 GPL19036 GPL20134

15 Samples

Download data: TXT

(Submitter supplied) Interspecies coaggregation promotes transcriptional changes of oral bacteria, contributing to the development of structurally balanced biofilms as well as oral diseases such as periodontitis. Streptococcus gordonii (S. gordonii) is an early colonizer of the oral cavity, and Fusobacterium nucleatum (F. nucleatum) may act as a bridge adhering to both early and late oral colonizers. These two species were commonly detected in healthy and periodontitis-diseased oral sites and could interact with immune cells such as macrophages. more...

Organism:

Streptococcus gordonii str. Challis substr. CH1; Fusobacterium polymorphum

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29567 GPL29569 GPL29568

9 Samples

Download data: FNA, GFF, TXT

(Submitter supplied) Multiple levels of interkingdom signaling have been implicated in maintaining the ecological balance between Candida albicans and commensal streptococci to assure a state of oral health. To better understand the molecular mechanisms involved in the initial streptococcal response to the presence of C. albicans that can initiate oral surface colonization and biofilm formation, hypha-forming cells were incubated with Streptococcus gordonii cells for 30 minutes to assess the streptococcal transcriptome response. more...

Organism:

Streptococcus gordonii

Type:

Expression profiling by array

Platform:

GPL20180

2 Samples

Download data: TXT

(Submitter supplied) In the oral biofilm, the mitis streptococci are among the first group of organisms to colonize the tooth surface. Their proliferation is thought to be an important factor required for antagonizing the growth of cariogenic species such as Streptococcus mutans. In this study, we used a 3-species mixed culture to demonstrate that another ubiquitous early colonizing species, Veillonella parvula, could greatly impact the competitive outcome of a mixed culture of S. more...

Organism:

Streptococcus mutans UA159; Streptococcus mutans

Type:

Expression profiling by array

Platform:

GPL4769

8 Samples

Download data: CEL

(Submitter supplied) Streptococcus gordonii, an important primary colonizer of dental plaque biofilm, specifically binds to salivary amylase via the surface-associated amylase-binding protein A (AbpA). We hypothesized that amylase binding to S. gordonii modulates expression of chromosomal genes, which could influence bacterial survival and persistence in the oral cavity. Gene expression profiling by microarray analysis was performed to detect differentially expressed genes in S. more...

Organism:

Streptococcus gordonii str. Challis

Type:

Expression profiling by array

Platform:

GPL14352

4 Samples

Download data: TXT

(Submitter supplied) Tn-Seq was used to identify S. gordonii genes that confer fitness during cooperative growth with P. gingivalis in a murine abscess model.

Organism:

Streptococcus gordonii

Type:

Other

Platform:

GPL28646

15 Samples

Download data: TXT

(Submitter supplied) The mechanisms through which oral commensal bacteria mitigates uncontrolled inflammatory responses in the oral mucosa remain unknown. Here we evaluated the ability of S. gordonii to stimulate the expression of miRNAs in oral epithelial cells with potential to target chemokine expression. The human oral epithelial cell line (OKF6) was exposed to different MOIs of S. gordonii for 24h and expression analysis of miRNAs performed using the Affymetrix platform.

Organism:

synthetic construct; Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL19117

8 Samples

Download data: CEL