GEO DataSets

(Submitter supplied) Transcript profiling was performed by Affymetrix microarray analysis and SAGE to characterize changes in gene expression in the bovine mammary gland in response to 4× versus 2× daily milking during the first week of lactation. These changes in gene expression may contribute to the increased milk production observed in response to increased milking frequency. Our results indicate that increased milking frequency alters expression of genes in the mammary gland related to increased extracellular matrix remodeling, neovascularization, metabolism, cell proliferation and apoptosis. more...

Organism:

Bos taurus

Type:

Expression profiling by array; Expression profiling by SAGE

Platforms:

GPL2112 GPL223

9 Samples

Download data: CEL

(Submitter supplied) Milking dairy cows four times daily (4X) instead of twice daily (2X) during early lactation stimulates an increase in milk yield that partly persists through late lactation; however, the mechanisms behind this response are unknown. We hypothesized that the acute mammary response to regular milkings would be transient and would involve different genes from those that may be specifically regulated in response to 4X. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS4009

Platform:

GPL2112

14 Samples

Download data: CEL

Analysis of mammary biopsies from lactating cows assigned to unilateral frequent milking (2X daily of left udder half, 4X daily of right udder half). Biopsies obtained at 5 days in milk, immediately after the 4X udder half had been milked, or 2.5 h after both udder halves had last been milked.

Organism:

Bos taurus

Type:

Expression profiling by array, transformed count, 2 other, 2 protocol, 7 specimen, 3 time sets

Platform:

GPL2112

Series:

GSE27380

14 Samples

Download data: CEL

(Submitter supplied) BACKGROUND: The lactating mammary gland responds to changes in milking frequency by modulating milk production. This response is locally regulated and, in dairy cows, the udder is particularly sensitive during early lactation. Relative to cows milked twice-daily throughout lactation, those milked four-times-daily for just the first 3 weeks of lactation produce more milk throughout that lactation. We hypothesized that the milk yield response would be associated with increased mammary cell turnover and changes in gene expression during frequent milking and persisting thereafter. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

36 Samples

Download data: CEL

(Submitter supplied) Once daily milking reduces milk yield and alters mammary transcriptome resulting in a decrease in milk protein synthesis as well as an induction of the apoptotic signaling networks. A local regulation due to milk stasis in the tissue could contribute to this effect but such mechanisms have not yet been described. To challenge this hypothesis, cows were milked unilaterally, once daily on one udder half and twice daily on the other one, and variations in gene expression were studied in biopsies as well as in mammary epithelial cells (MEC) shed into milk during the lactation process (milk MEC). more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6694

10 Samples

Download data: TXT

(Submitter supplied) Regulation of milk synthesis and secretion is controlled mostly through local (intra-mammary) mechanisms. To gain insight into the molecular pathways comprising this response, an analysis of mammary gene expression was conducted in 12 lactating cows shifted from twice daily to once daily milking. Tissues were sampled by biopsy from adjacent mammary quarters of these animals during the two milking frequencies, allowing changes in gene expression to be assessed within each animal. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL8618

12 Samples

Download data: TXT

(Submitter supplied) The ruminant liver has multiple roles in the dairy cow and many of these are crucial in nutrient supply during lactation. Reduced feed intake alters the expression of many genes and pathways in the liver, inducing a period of negative energy balance. Once-daily milking is a management strategy to reduce the effects of periods of negative energy balance so the objective of this study was to determine if once-daily milking altered hepatic gene transcription during a period of negative energy balance induce by caloric restriction. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL9712

45 Samples

Download data: TXT

(Submitter supplied) The objective was to evaluate the effect of nutrient restriction and intramammary lipopolysaccharide (LPS) challenge on mammary gland (MG) gene expression in early lactation cows. At 24 ± 3 d in milk, multiparous cows were either allowed to continue ad libitum intake of a lactation diet (CON, n = 6), or the ration was diluted with barley straw (48% DM) for 4 d (RES, n = 6). On d 3, one healthy rear mammary quarter was infused with 50 µg of LPS. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11648

12 Samples

Download data: TXT

(Submitter supplied) In this study, two small RNA libraries were constructed using dry period and peak lactation dairy goat mammary gland tissues and sequenced by the Illumina Solexa high-throughput sequencing system. A total of 346 conserved and 95 novel miRNAs were identified in the dairy goat. The expression of miRNAs was confirmed by qRT-PCR in nine tissues and the mammary gland during development cycles. In addition, several candidate miRNAs that may be involved in mammary gland development and lactation were found by the comparison of miRNA expression profiles among different tissue and developmental stages of the mammary gland. more...

Organism:

Capra hircus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15473

2 Samples

Download data: TXT

(Submitter supplied) Animal nutrition considerably affects milk composition that influences its nutritional quality. Milk component synthesis and secretion by the mammary gland involve expression of a large number of genes whose nutritional regulation remains poorly defined. In this study, we examined the effect of food deprivation (FD) on the expression of 8379 genes in caprine mammary gland using a bovine oligonucleotide microarray. more...

Organism:

Bos taurus; Capra hircus

Type:

Expression profiling by array

Platform:

GPL4594

12 Samples

Download data: TXT

(Submitter supplied) Bovine mammary stem cells (MaSC) are a source of ductal and lobulo-alveolar tissue during development of mammary gland and its remodeling in repeating lactation cycles. We hypothesize that the number of MaSC, their molecular properties and interactions with their niche may be essential to determine the mammogenic potential in heifers. To verify this hypothesis we compared the number of MaSC and transcriptomic profile in mammary tissue of 2-year-old, non-pregnant dairy (Holstein-Friesian) and beef (Limousin) heifers. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11649

4 Samples

Download data: TXT

(Submitter supplied) Liver and mammary gland are among the most prominent organs during lactation in dairy cows. With the purpose to understand 1) the adaptation of liver and mammary to different levels of forage/concentrate ration (60:40 vs. 40:60) and 2) the crosstalk between the two organs during lactation, a transcriptome analysis was performed on liver and mammary tissues of 10 primiparous dairy cows in mid-lactation. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11649

20 Samples

Download data: TXT

(Submitter supplied) Our overall objective is to identify key differences in gene expression signaling pathways in the epithelial and intralobular stromal compartments during prepartum mammary remodeling and development in the dry cow.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

12 Samples

Download data: CEL

(Submitter supplied) We report the application of Solexa high-throuthput sequencing technology for miRNA differential expression profiles. Using an optimized data analysis workflow, we mapped about 30 million sequence reads per sample to Ovis aries genome. The results were validated by qRT-PCR. Our study represents the first detailed analysis of miRNA expression profiles in goat mammary gland tissues, with biologic replicates generated by RNA-seq technology.

Organism:

Capra hircus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15473

2 Samples

Download data: FA, TXT

(Submitter supplied) The role of chromatin in mammary gland development and differentiation has not been defined. Here we have studied the changes in chromatin conformation in the mammary gland during the lactation cycle at milk protein gene loci, whose gene expression marks functional differentiation of the mammary gland.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9250

12 Samples

Download data: TXT

(Submitter supplied) The role of chromatin in mammary gland development and differentiation has not been defined. Here we have studied the changes in chromatin conformation in the mammary gland during the lactation cycle at milk protein gene loci, whose gene expression marks functional differentiation of the mammary gland

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9250

10 Samples

Download data: TXT

(Submitter supplied) The effects of a negative energy balance induced by a nutritional restriction on mammary gland were evaluated using the 4x44K bovine microarray from Agilent

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11648

12 Samples

Download data: TXT

(Submitter supplied) Effect of feed restriction in mid lactation Holstein (H) and Montbéliarde (M) cows on mammary glande miRNA profil.

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL23295

24 Samples

Download data: TXT

(Submitter supplied) Here, we show that functional loss of a single gene is sufficient to confer constitutive milk protein production and protection against mammary tumor formation. Caveolin-3 (Cav-3), a muscle-specific caveolin-related gene, is highly expressed in striated and smooth muscle cells. We demonstrate that Cav-3 is also expressed in myoepithelial cells within the mammary gland. To determine if genetic ablation of Cav-3 expression affects adult mammary gland development, we next studied the phenotype(s) of Cav-3 (-/-) null mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3533

Platform:

GPL1261

6 Samples

Download data: CEL

Analysis of caveolin-3 (Cav-3) deficient mammary glands from virgin females. Cav-3 deficient virgin mammary glands develop lobulo-alveolar hyperplasia, akin to the changes normally observed during pregnancy and lactation. Results provide insight into the molecular basis of this Cav-3 null phenotype.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE12881

6 Samples

Download data: CEL