GEO DataSets

(Submitter supplied) Microarrray experiments were performed in order to identify genes whose expression is altered under hypoxic conditions and to determine whether these changes are dependant on the p53 and HIF tanscription factors. In order to determine HIF dependance, isogenic RCC4 cell lines were used that were either wildtype of null for vhl, the gene responsible for degrading HIF under normoxic conditions. In order to determine p53 dependance, isogenic HCT116 and H1299 cell lines that were wildtype or null for p53 were used. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1772

Platform:

GPL3423

30 Samples

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Analysis of cell lines lacking VHL or p53 following exposure to hypoxia. VHL is the gene responsible for degrading transcription factor HIF-1. p53 null mutants examined to identify genes whose expression under hypoxia depends on p53. Results identify genes regulated by HIF-1.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 cell line, 4 genotype/variation, 4 stress, 8 time sets

Platform:

GPL3423

Series:

GSE4186

30 Samples

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(Submitter supplied) Activation of glycolytic genes by HIF-1 is considered critical for metabolic adaptation to hypoxia. We found that HIF-1 also actively suppresses glucose metabolism through the tricarboxylic acid cycle (TCA) by directly trans-activating the gene encoding pyruvate dehydrogenase kinase 1 (PDK1). PDK1 inactivates the TCA cycle enzyme, pyruvate dehydrogenase (PDH), which converts pyruvate to acetyl-CoA. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1807

Platform:

GPL570

4 Samples

Download data: CEL, EXP

Expression profiling of B lymphocyte P493-6 cells subjected to hypoxia. Cells incubated in 0.1% oxygen for 29 hours, the point of highest expression of hypoxia-inducible factor 1 (HIF-1). Results identify hypoxia responsive genes in P493-6 cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 stress sets

Platform:

GPL570

Series:

GSE4086

4 Samples

Download data: CEL, EXP

(Submitter supplied) To identify new primary HIF target genes we cultured HeLa cells under short period (6 hours) of mild hypoxia (1% oxygen)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

4 Samples

Download data: TXT

(Submitter supplied) The overall aim of the present work was to identify MTG16 functions in leukemia cells. Alterations in quantity of the MTG16 co-repressor might affect gene regulation and cell metabolism in malignant cells. Differentiated cells secure energy for cellular homeostasis largely by mitochondrial oxidation. Whereas, mature cells, proliferating tumour cells including leukemia cells depend on glycolysis and mitochondrial respiration may be low even in oxygen–rich environments.The same signal transduction pathways that govern cell proliferation give instructions for nutrient uptake and co-regulate metabolic processes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

6 Samples

Download data: CEL, TXT