GEO DataSets

(Submitter supplied) The goal of this study was to assess whether low shear-modeled microgravity (LSMMG) effects yeast genomic expression patterns using the powerful tool of whole genome microarray hybridization. We determined changes in the yeast model organism, Saccharomyces cerevisisae, when grown in LSMMG using the rotating High Aspect Ratio Vessel (HARV). A significant number of genes were up- or down-regulated by at least two fold in cells that were grown for 5 generations or 25 generations in HARVs. more...

Organism:

Saccharomyces cerevisiae; Schizosaccharomyces pombe

Type:

Expression profiling by array

Dataset:

GDS1687

Platform:

GPL2529

12 Samples

Download data

Analysis of wild type BY4743 cells grown in a low-shear modeled microgravity environment (LSMMG). LSMMG environment produced using a high aspect rotating vessel. Results provide insight into the response to the microgravity environment experienced during space flight.

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae

Type:

Expression profiling by array, transformed count, 2 age, 2 stress sets

Platform:

GPL2529

Series:

GSE4136

12 Samples

Download data

(Submitter supplied) Anticipating the risk for infectious disease during space exploration and habitation is a critical factor to ensure safety, health and performance of the crewmembers. As a ubiquitous environmental organism that is occasionally part of the human flora, Pseudomonas aeruginosa could pose a health hazard for the immuno-compromised astronauts. In order to gain insights in the behavior of P. aeruginosa in spaceflight conditions, two spaceflight-analogue culture systems, i.e. more...

Organism:

Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1

Type:

Expression profiling by array

Platform:

GPL84

8 Samples

Download data: CEL

(Submitter supplied) This study explores the connection between changes in gene expression and the genes that determine strain survival during suspension culture, using the model eukaryotic organism, Saccharomyces cerevisiae. The Saccharomyces cerevisiae homozygous diploid deletion pool, and the BY4743 parental strain were grown for 18 hours in a rotating wall vessel, a suspension culture device optimized to minimize the delivered shear. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platforms:

GPL2544 GPL90

12 Samples

Download data

(Submitter supplied) Comparison of the transcriptomes of Saccharomyces cerevisiae wild type FY23 and a PDE2 deletion mutant DJ28. Keywords = PDE2 Keywords = Ras/cAMP pathway Keywords: other

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL422

10 Samples

Download data

(Submitter supplied) affymetrix gene expression data over three successive metabolic cycles Keywords: yeast, time course, metabolic cycle

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS2267

Platform:

GPL90

36 Samples

Download data

Analysis of nutrient-limited continuous-culture cells at 12 25-minute intervals for 3 cycles. Cells grown under such conditions exhibit robust, periodic cycles in the form of respiratory bursts. Results provide insight into molecular mechanisms responsible for controlling metabolic oscillations.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, transformed count, 3 protocol, 12 time sets

Platform:

GPL90

Series:

GSE3431

36 Samples

Download data

(Submitter supplied) transcriptome response of Arabidopsis cultivar Columbia etiolated seedlings and undifferentiated tissue culture cells to the spaceflight environment We address a key baseline question of whether gene expression changes are induced by the orbital environment, and then we ask whether undifferentiated cells, cells presumably lacking the typical gravity response mechanisms, perceive spaceflight. Arabidopsis seedlings and undifferentiated cultured Arabidopsis cells were launched in April, 2010, as part of the BRIC-16 flight experiment on STS-131. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL198

24 Samples

Download data: CEL, TXT

(Submitter supplied) The purpose of this study was to search for microgravity-sensitive genes, specifically for apoptotic genes influenced by the microgravity environment and other genes related to immune response. Keywords: stress response

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1806

Platform:

GPL96

6 Samples

Download data

Analysis of activated T-cells subjected to modeled microgravity. The study seeks to identify microgravity sensitive genes involved in apoptosis and the immune response. Microgravity is an environment of little net gravitational force that is experienced in spaceflight and by free-falling objects.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 protocol sets

Platform:

GPL96

Series:

GSE4209

6 Samples

Download data

(Submitter supplied) Transcriptional regulation of branched-chain amino acid metabolism in Saccharomyces cerevisiae involves two key regulator proteins, Leu3p and Gcn4p. Leu3p is a pathway-specific regulator, known to regulate six genes involved in branched-chain amino acid metabolism and one gene in nitrogen assimilation. Gcn4p is a global regulator, involved in the general response to amino acid and purine starvation. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS1103

Platform:

GPL90

12 Samples

Download data

Analysis of leu3 mutant grown in either limited ethanol or limited ammonium media. Leu3p regulates a gene involved in nitrogen assimilation and six genes involved in branched chain amino acid metabolism. Results provide insight into the role of Leu3p in gene regulation.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 2 genotype/variation, 2 growth protocol sets

Platform:

GPL90

Series:

GSE2076

12 Samples

Download data

(Submitter supplied) Cells must adjust their gene expression in order to compete in a constantly changing environment. Two alternative strategies could in principle ensure optimal coordination of gene expression with physiological requirement. First, the internal physiological state itself could feedback to regulated gene expression. Second, the expected physiological state could be inferred from the external environment, using evolutionary-tuned signaling pathways. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL4567

95 Samples

Download data: XLS

(Submitter supplied) We used RNA-seq to monitor mRNA levels of all genes in response to hypoxia of wild-type yeast, S. cerevisiae (strain yMH914 with wildtype HAP1). To gain insights into how gene expression changes over time, cells were subjected to 100% nitrogen gas and collected after 0,5,10,30,60,120,180, and 240 minutes. Total RNA was extracted and mRNAs were enriched by polyA selection. The cDNA was prepared into a sequencing library, multiplexed and single-end sequenced by an Illumina HiSeq 2500 sequencer. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17342

24 Samples

Download data: TAB

(Submitter supplied) A total cellular RNA analysis was performed to determine differential gene expression between wild-type and kap108∆ S. cerevisiae cells under both normal and oxidative stress conditions. Goal was to determine which genes are differentially expressed in the absence of the Kap108 importin, and to see how that differential expression changes with the addition of oxidative stress. This information can then be used to help elucidate the function and transport cargoes of Kap108, which are currently unknown.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL9294

12 Samples

Download data: TXT

(Submitter supplied) Ccw12p is a cell surface mannoprotein required for cell wall stability. To investigate the compensation mechanism after CCW12 deletion we analysed the global gene expression in ccw12 mutant cells.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL90

4 Samples

Download data: CEL

(Submitter supplied) DLD-1 and MOLT-4 cell lines were cultured in a Rotating cell culture system to simulate microgravity and mRNA expression profile was observed in comparison to Static controls Simulated microgravity affected the solid tumor cell line DLD-1 markedly which showed a higher percentage of dysregulated genes compared to the hematological tumor cell line, MOLT-4. Microgravity affects the cell cycle of DLD-1 cells and disturbs expression of cell cycle regulatory gene networks. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL15207

8 Samples

Download data: CEL

(Submitter supplied) Physical forces associated with spaceflight and spaceflight analogue culture regulate a wide range of physiological responses by both bacterial and mammalian cells that can impact infection. However, our mechanistic understanding of how these environments regulate host-pathogen interactions in humans is poorly understood. Using a spaceflight analogue low fluid shear culture system, we investigated the effect of Low Shear Modeled Microgravity (LSMMG) culture on the colonization of Salmonella Typhimurium in a 3-D biomimetic model of human colonic epithelium containing macrophages. more...

Organism:

Homo sapiens; Salmonella enterica subsp. enterica serovar Typhimurium

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL28222 GPL28223 GPL21697

72 Samples

Download data: TXT

(Submitter supplied) The number and type of synthetic chemicals that are being produced worldwide continues to increase significantly. While these industrial chemicals provide numerous benefits, there is no doubt that some have potential to damage the environment and health. Toxicity must be evaluated and use must be carefully controlled and monitored in order to minimize potential damage. DNA microarray technology has become an important new technique in toxicology. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

4 related Platforms

27 Samples

Download data: GPR

(Submitter supplied) Pyrimethanil (PYR) is a world-wide used fungicide approved for use in plant protection products in Agriculture, and with some (eco)toxicological concerns.We aimed at finding molecular biomarkers in the model yeast Saccharomyces cerevisiae that may be used to predict potential cytotoxic effects of this xenobiotic while providing mechanistic clues possibly relevant for experimentally less accessible non-target eukaryotes. more...

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL2529

9 Samples

Download data: CEL