Similar studies for GEO DataSets (Select 200002015) - GEO DataSets

Select item 2000020151.

Intestinal epithelial cells transfected with Ras and treated with TGF-beta-1

(Submitter supplied) RIE (rat intestinal epithelial) cells treated with 2 ng/ml of TGF-beta-1 for 1 hr were compared to control cells. The experiment was performed under identical conditions four times: RIE vs RIE - TGF-beta #1-#4. RIE-Ras cells were established by stable transfection of the parental cells with pSV2-H-Ras(12V) which contain human sequences encoding the constitutively active H-Ras(12V) protein. RIE-Ras cells treated with 2 ng/ml of TGF-beta-1 for 1 hr were compared to control cells. more...

Organism:: Rattus norvegicus

Type:: Expression profiling by array

Platform:: GPL890
8 Samples

Download data: TIFF

Series

Accession:: GSE2015

ID:: 200002015

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000082402.

EMT initiation in MCF10A cells subjected to ionizing radiation and treatment with transforming growth factor beta-1.

(Submitter supplied) Transforming growth factor beta-1 (TGFbeta) is a tumor suppressor during the initial stage of tumorigenesis, but it can switch to a tumor promoter during neoplastic progression. Ionizing radiation (IR), both a carcinogen and a therapeutic agent, induces TGFbeta activation in vivo. We now show that IR sensitizes human mammary epithelial cells (HMEC) to undergo TGFbeta-mediated epithelial to mesenchymal transition (EMT). more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL3921
15 Samples

Download data: CEL, CHP

Series

Accession:: GSE8240

ID:: 200008240

Select item 2000019553.

Gene expression changes induced by K-ras oncogene in the human pancreatic duct epithelial cell line (H6c7)

(Submitter supplied) k-ras oncogene was transduced into the HPV16-E6E7 immortalized normal human pancreatic duct epithelial cell line (H6c7) to generate a stable K-ras oncogene-expressing cell line (H6c7-Kr). The gene expression profile of the H6c7-Kr cells was compared to that of H6c7 cells. We have excluded (subtracted) genes that were deregulated by transduction of the construct used for K-ras oncogene expression. Keywords: parallel sample

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL96
2 Samples

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Series

Accession:: GSE1955

ID:: 200001955

Select item 2000051704.

TGFbeta- and IMR induced epithelial cell differentiation in a three-dimensional intestinal crypt-villus axis model

(Submitter supplied) The three-dimensional epithelial differentiation model, in which T84 epithelial cells are induced to differentiate either with TGFbeta1 or IMR-90 mesenchymal cell-secreted soluble factors, is previously shown to model differentiation seen in jejunal crypt-villus axis (Halttunen et al. 1996). The gene expression changes were studied in fully undifferentiated and fully differentiated three-dimensional T84 cultures with cDNA microarray method. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL3928
9 Samples

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Series

Accession:: GSE5170

ID:: 200005170

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000151265.

A gene expression signature associated with K-Ras addiction reveals regulators of EMT and tumor cell survival

(Submitter supplied) K-Ras mutations occur frequently in epithelial cancers. Using shRNAs to deplete K-Ras in lung and pancreatic cancer cell lines harboring K-Ras mutations, two classes were identified—lines that do or do not require K-Ras to maintain viability. Comparing these two classes of cancer cells revealed a gene expression signature in K-Ras-dependent cells, associated with a well-differentiated epithelial phenotype, which was also seen in primary tumors. more...

Organism:: Homo sapiens

Type:: Expression profiling by array; Genome binding/occupancy profiling by SNP array

Platforms:: GPL1352 GPL3718 GPL3720
60 Samples

Download data: CEL, TXT

Series

Accession:: GSE15126

ID:: 200015126

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000991006.

Gene expression profiels from organoids.

(Submitter supplied) Recent studies have suggested increased plasticity of differentiated cells within the intestine to act both as intestinal stem cells and tumour initiating cells. However, little is known of the processes that regulate this plasticity. Our previous work has shown that activating mutations of Kras or the NF-kB pathway can drive dedifferentiation of intestinal cells lacking Apc. To investigate this process further, we profiled organoids by gene expression analysis. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
6 Samples

Download data: CEL

Series

Accession:: GSE99100

ID:: 200099100

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000990777.

Gene Expression profiles from allograft tumours

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
6 Samples

Download data: CEL

Series

Accession:: GSE99077

ID:: 200099077

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2001820198.

Epithelial TGFβ activation engages growth-factor signalling to circumvent cell death and drive intestinal tumorigenesis with aggressive features.

(Submitter supplied) The pro-tumourigenic role of epithelial TGFβ in colorectal cancer (CRC) has been controversial. Here we identify a cohort of aggressive ‘bad acting’ early-stage (T1) disseminating tumours characterised by high cell-intrinsic TGFβ signalling emanating from the epithelium, not stroma. To address its functional significance, we activated TGFβ signalling in the murine intestinal epithelium either alone or in concert with the common tumour suppressive and oncogenic mutations found in CRC, namely Apc and Kras. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL19057
47 Samples

Download data: CSV

Series

Accession:: GSE182019

ID:: 200182019

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000949379.

Generation of KRAS signatures using immortalized isogenic lung cells.

(Submitter supplied) We generated RNAseq profiles from Small Airway Epithelial Cells (SALE) expressing either KRAS G12V or GFP control.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18573
10 Samples

Download data: TXT

Series

Accession:: GSE94937

ID:: 200094937

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 20008470610.

Experimental validation of candidate regulators of KRAS transcriptional components.

(Submitter supplied) We sequenced mRNA from HCC44 and YAPC cell lines stably expressing Cas9, and infecting with indicated gRNAs against regulators of KRAS transcriptional components.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18573
21 Samples

Download data: GCT

Series

Accession:: GSE84706

ID:: 200084706

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 20003066711.

Microarray profiling reveals Integrated Stress Response is activated by Halofuginone in mammary epithelial cells

(Submitter supplied) The small molecule Halofuginone (HF) is a potent regulator of extracellular matrix (ECM ) gene expression and is unique in its therapeutic potential. While the basis for HF effects is unknown, inhibition of TGFb signaling and activation of the AAR have been linked to HF transcriptional control of a number of ECM components and amelioration of fibrosis and alleviation of autoimmune disease by regulation of Th17 cell differentiation, respectively. more...