GEO DataSets

(Submitter supplied) Reticulocytes were purified from fresh blood samples obtained from 10 healthy adult volunteers (5 women and 5 men) after informed consent. The donors had normal blood cell counts, blood smears, hemoglobin electrophoresis, red cells membrane resistance tests and no biological evidence of hemolysis. Whole blood samples were centrifuged, the supernatant and the buffy coat containing white blood cells (WBC) and platelets were removed. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL6

1 Sample

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(Submitter supplied) Gene expression profiles of somatic cells of fetal and adult testis. To identify genes developmentally regulated in the somatic cells of the testis, serial analysis of gene expression (SAGE) has been used to generate gene expression profiles from these cells in the fetal and adult mouse. To avoid germ cell transcripts, a fetal SAGE library was generated from germ cell-free fetal Wv/Wv mice, and an adult SAGE library was generated from adult testes depleted of germ cells with busulfan. more...

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

2 Samples

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(Submitter supplied) Evaluation of extraocular muscle (EOM) critical period by expression profiling in dark reared rat. Data form part of publication: Investigative Ophthalmology and Visual Sciences 44: 3842-3855, 2003. Keywords: other

Organism:

Rattus norvegicus

Type:

Expression profiling by SAGE

Platform:

GPL23

2 Samples

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(Submitter supplied) SAGE analysis of genes expressed in rat brain microvessels. Keywords: Novel gene identification

Organism:

Rattus norvegicus

Type:

Expression profiling by SAGE

Platform:

GPL23

1 Sample

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(Submitter supplied) computational analysis of transcriptomic information during erythroid development Keywords: erythroid differentiation

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2431

Platform:

GPL570

18 Samples

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Analysis of adult differentiating CD34+ hematopoietic progenitor cells at various time points up to 11 days of growth in serum-free medium containing erythropoietin, interleukin-3 and stem cell factor. Results provide insight into the molecular basis of erythropoiesis.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 6 time sets

Platform:

GPL570

Series:

GSE4655

18 Samples

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(Submitter supplied) The SAGE libraries were built from total mRNA populations in specific tissues, cells, mutation-specific populations, and all developmental stages of C. elegans. Tissue- and cell-specific libraries were generated from FACS-sorted cells marked by expression of specific promoter::GFP fusions Keywords = SAGE Keywords = LongSAGE Keywords = Caenorhabditis elegans Keywords = development Keywords = dauer Keywords: other

Organism:

Caenorhabditis elegans

Type:

Expression profiling by SAGE

Platforms:

GPL1267 GPL1268

17 Samples

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(Submitter supplied) Müller cells are the principal glial cells in the retina. Alterations in Müller cell behaviour are observed in retinal tissue from patients with proliferative diabetic retinopathy. The purpose of this study was to compare gene and protein expression profile of normal human Müller cells (NHMC) with two spontaneously human Müller cell lines generated from type 1 (HMCL-I) and type 2 (HMCL-II) diabetic donors using Serial Analysis Gene Expression (SAGE). more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL2750

3 Samples

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(Submitter supplied) These results provide valuable information for understanding the mechanisms of radiation-induced changes in gene expression.

Organism:

Bombyx mori

Type:

Expression profiling by SAGE

Platform:

GPL2095

2 Samples

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(Submitter supplied) Human bone marrow is a complex, diversified and well-organized hematopoietic network changing composition with age. The purpose of this study was to analyze variations in relative precursor B cell abundance in bone marrow with age by means of global gene expression profiling. RNA was isolated from composite bone marrow from 25 healthy children, adolescents and adults age 2 months to 28 years. As reference transcript for precursor B cells we used recombination activating gene RAG1 exploring the data for other transcripts showing the same profile as RAG1 with age. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4263

Platform:

GPL570

25 Samples

Download data: CEL

Analysis of composite bone marrow (BM) from healthy children and adults. Normal BM constitutes a complex tissue of blood progenitor cells changing in composition with age. Results provide insight into molecular mechanisms associated with changes in the total precursor B-cell compartment with age.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 25 age sets

Platform:

GPL570

Series:

GSE11504

25 Samples

Download data: CEL

(Submitter supplied) Libraries generated from microdissected kidney samples from the healthy pole of cancerous kidneys. Donors were from either sex. Libraries were generated using Sau3A I as anchoring enzyme. Data are provided after removal of linker derived sequences.

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by SAGE

Platforms:

GPL6 GPL275

9 Samples

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(Submitter supplied) 2 normal lung parenchyma SAGE libraries, generated from 2 pools of 4 individuals each Discussed in the study: IDENTIFICATION OF NOVEL LUNG GENES IN BRONCHIAL EPITHELIUM BY SERIAL ANALYSIS OF GENE EXPRESSION Kim M. Lonergan1, Raj Chari1, Ronald J. deLeeuw1, Ashleen Shadeo1, Bryan Chi1, Ming-Sound Tsao2, Steven Jones3, Marco Marra3, Victor Ling1, Raymond Ng1,4, Calum MacAulay5, Stephen Lam5 and Wan L. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

2 Samples

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(Submitter supplied) Triticum aestivum cultivars Scorpion 25 and Xi 19 were grown under both normal and hot/dry conditions. We compared the effect of these two growth conditions on these two closely related varieties.

Organism:

Triticum aestivum

Type:

Expression profiling by SAGE

Platform:

GPL7079

6 Samples

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(Submitter supplied) This library was generated from normal human thyroid tissue collected at routine autopsy. Standard SAGE protocol was used (Velculescu et al. Science 1995). Data from this library was published in: 'Pauws E, Moreno JC, Tijssen M, Baas F, de Vijlder JJ, Ris-Stalpers C. J Clin Endocrinol Metab. 2000 May;85(5):1923-7' Data was generated and analyzed at the laboratory of Pediatric Endocrinology, Academic Medial Center, Amsterdam, The Netherlands.

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

1 Sample

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(Submitter supplied) A transcriptome study in mouse hematopoietic stem cells was performed using a sensitive SAGE method, in an attempt to detect medium and low abundant transcripts expressed in these cells. Among a total of 31,380 unique transcript, 17,326 (55%) known genes were detected, 14,054 (45%) low-copy transcripts that have no matches to currently known genes. 3,899 (23%) were alternatively spliced transcripts of the known genes and 3,754 (22%) represent anti-sense transcripts from known genes.

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

1 Sample

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(Submitter supplied) SAGE libraries were prepared from mRNA of cultured bovine glomerular and aortic endothelial cells. Cells both cell lines were positive for acetylated LDL uptake and vWF expression, and were proven to be free of non-endothelial cells. Cultures were studied at passage 9-10. The cells were confluent, cultured in parallel, under identical conditions. Cells were plated on gelatin coated cell culture plastic in RPMI 1640 medium containing 10% FBS and streptomycin/penicillin. more...

Organism:

Bos taurus

Type:

Expression profiling by SAGE

Platform:

GPL223

2 Samples

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(Submitter supplied) RNA from circulating blood reticulocytes was utilized to provide a robust description of genes transcribed at the final stages of erythroblast maturation. After depletion of leukocytes and platelets, Affymetrix HG-U133Plus 2.0 arrays were hybridized with probe from total RNA isolated from blood sampled from 6 umbilical cords and 6 healthy adult humans.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3814

Platform:

GPL570

12 Samples

Download data: CEL

(Submitter supplied) MicroRNA are ~22nt-long small non-coding RNA that negatively regulate protein expression through mRNA degradation or translational repression in eukaryotic cells. Based upon their importance in regulating development and terminal differentiation in model systems, erythrocyte microRNA profiles were examined at birth and in adults to determine if changes in their abundance coincide with the developmental phenomenon of hemoglobin switching. more...

Organism:

Homo sapiens; human gammaherpesvirus 4

Type:

Non-coding RNA profiling by array

Platform:

GPL8949

8 Samples

Download data: GPR

Comparison of reticulocytes from adult peripheral blood and umbilical cord blood. Hemoglobin (Hb) composition switches around the time of birth from fetal Hb to adult Hb. Together with microRNA profiling, results provide insight into the role of microRNAs in fetal-to-adult Hb switching.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 development stage sets

Platform:

GPL570

Series:

GSE17639

12 Samples

Download data: CEL