GEO DataSets

(Submitter supplied) RNA from vastus lateralis of healthy young (21-31 year old) and older (62-77 year old) men. Signal data normalized to mean intensity of 500 over all probes sets. Analysis done with Affymetrix Microarray Suite 5.0 software. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS156

Platform:

GPL91

12 Samples

Download data: CEL

Comparison of gene expression in vastus lateralis skeletal muscle biopsies in healthy young (21-31 year old) and older (62-77 year old) men. Sample RNAs isolated from individuals and pooled into groups of 8.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 age sets

Platform:

GPL91

Series:

GSE80

12 Samples

Download data: CEL

(Submitter supplied) vastus lateralis biopsies were obtained from healthy subjects, either young adults (20-29 yr old) or older (65-75 yr old) Keywords = muscle Keywords = aging Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS287 GDS288

Platforms:

GPL96 GPL97

30 Samples

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Comparison of gene expression in vastus lateralis skeletal muscle biopsies in healthy young (21-27 year old) and older (67-75 year old) men.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 age sets

Platform:

GPL97

Series:

GSE362

15 Samples

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Comparison of gene expression in vastus lateralis skeletal muscle biopsies in healthy young (21-27 year old) and older (67-75 year old) men

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 age sets

Platform:

GPL96

Series:

GSE362

15 Samples

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(Submitter supplied) Both spotted long oligonucleotide arrays and Affymetrix GeneChips were used to measure differential gene expression in two RNA samples (K562 erythroleukemia RNA and the Stratagene Universal Human Reference RNA). For Affymetrix technology, the two RNAs were analyzed separately. There are two replicates for K562 RNA (GSM4843 and GSM4844) and three for the Stratagene Universal reference (GSM4845-GSM4847). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS222 GDS223

Platforms:

GPL273 GPL8300 GPL274

23 Samples

Download data: CEL

Part of a comparison of spotted long oligonucleotide arrays with in situ synthesized 25-mer arrays. Examination of amplified and unamplified K562 erythroleukemia RNA and Stratagene Universal Human Reference RNA.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 cell type sets

Platform:

GPL8300

Series:

GSE344

5 Samples

Download data: CEL

Part of a comparison of spotted long oligonucleotide arrays with in situ synthesized 25-mer arrays. Examination of amplified and unamplified K562 erythroleukemia RNA and Stratagene Universal Human Reference RNA.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 3 protocol sets

Platform:

GPL273

Series:

GSE344

14 Samples

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(Submitter supplied) We analysed the transcriptional effects of the TOR2-controlled signaling function using a genome-wide microarray approach in yeast. In S. cerevisiae, TOR2 has two essential signaling functions. One, shared with TOR1, is required for translation initiation, transcription, and cell growth in response to the presence of nutrients. The second is unique to TOR2, and functions in cell-cycle-dependent actin polarization and possibly in transcription. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS922

Platform:

GPL90

12 Samples

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Comparison of total transcription profiles for temperature-sensitive TOR2 mutant strain SH121 to its isogenic wild type counterpart SH100. Results indicate that TOR2 inactivation leads to enhanced transcription of Gcn4-controlled target genes.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 2 genotype/variation, 3 time sets

Platform:

GPL90

Series:

GSE1814

12 Samples

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(Submitter supplied) Background:Alternative splicing and isoform level expression profiling is an emerging field of interest within genomics. Splicing sensitive microarrays, with probes targeted to individual exons or exon-junctions, are becoming increasingly popular as a tool capable of both expression profiling and finer scale isoform detection. Despite their intuitive appeal, relatively little is known about the performance of such tools, particularly in comparison with more traditional 3’ targeted microarrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL5175 GPL5188

40 Samples

Download data: CEL

(Submitter supplied) Background:Alternative splicing and isoform level expression profiling is an emerging field of interest within genomics. Splicing sensitive microarrays, with probes targeted to individual exons or exon-junctions, are becoming increasingly popular as a tool capable of both expression profiling and finer scale isoform detection. Despite their intuitive appeal, relatively little is known about the performance of such tools, particularly in comparison with more traditional 3’ targeted microarrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5188

20 Samples

Download data: CEL

(Submitter supplied) Background:Alternative splicing and isoform level expression profiling is an emerging field of interest within genomics. Splicing sensitive microarrays, with probes targeted to individual exons or exon-junctions, are becoming increasingly popular as a tool capable of both expression profiling and finer scale isoform detection. Despite their intuitive appeal, relatively little is known about the performance of such tools, particularly in comparison with more traditional 3’ targeted microarrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5175

20 Samples

Download data: CEL

(Submitter supplied) #G017 Burn/Trauma Study. This is a Glue Grant Study comparison between gene expression analyses performed in eight trauma/burn subjects using either a buffy coat isolation of whole blood or using the PAXgene system Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS917

Platform:

GPL96

16 Samples

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Comparison of RNA isolation from whole blood using the PAXgene system to that from leukocytes using the buffy coat procedure. Blood of healthy subjects and trauma patients examined. Results indicate that RNA isolated from whole blood produced more noise than that from leukocytes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 protocol, 2 tissue sets

Platform:

GPL96

Series:

GSE1343

16 Samples

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(Submitter supplied) There is an increasing interest on the role of Alternative splicing (AS) in different pathologies. The Affymetrix Human Transcriptome Array (HTA 2.0) can be used to explore AS very efficiently. However, the interpretation software provided by its vendor (TAC 3.0) does not fully exploit its potential and can only be applied to case-control studies. EventPointer is an R package to identify Alternative Splicing events using HTA 2.0 arrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL21339

27 Samples

Download data: CEL

(Submitter supplied) Microarray technology provides a powerful tool for defining gene expression profiles of airway epithelium that lend insight into the pathogenesis of human airway disorders. The focus of this study was to establish rigorous quality control parameters to ensure that microarray assessment of the airway epithelium is not confounded by experimental artifact. Samples (total n=223) of trachea, large and small airway epithelium were collected by fiberoptic bronchoscopy of 144 individuals (42 healthy non-smokers, 49 healthy smokers, 11 symptomatic smokers, 22 smokers with lone emphysema with normal spirometry, and 20 smokers with COPD) were processed and hybridized to Affymetrix HG-U133 2.0 Plus microarrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

223 Samples

Download data: CEL, CHP

(Submitter supplied) Purified DNA of some 4600 overlapping shotgun clones in vector pCR-BluntII-TOPO (Invitrogen, Groningen, The Netherlands) was used as template in standard PCR amplification with the vector-specific primer pair d(TCGGATC CACTAGTAACG) and d(GGCCGCCAGTGTGATG). Shotgun cloning had been performed according to the procedures described by the vector's manufacturer. Each PCR reaction was individually checked by agarose gel electrophoresis. more...

Organism:

Pseudomonas putida

1 Series

6 Samples

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(Submitter supplied) Knowledge of the biological and technical variation for fermentor-grown Aspergillus niger cultures is needed to design DNA microarray experiments properly. We cultured A. niger in batch-operated fermentor vessels and induced with D-xylose. Transcript profiles were followed in detail by qPCR for 8 genes. A variance components analysis was performed on these data to determine the origin and magnitude of variation within each process step for this experiment. more...

Organism:

Aspergillus niger; Aspergillus niger CBS 513.88

Type:

Expression profiling by array

Platform:

GPL6758

7 Samples

Download data: CEL

(Submitter supplied) Variance in microarray studies has been widely discussed as a critical topic of the identification of differentially expressed gene; however, few studies have addressed the influence of estimating variance. To break intra- and inter-individual variance in clinical studies down to three levels: technical, anatomic, and individual, we designed experiments and algorithms to investigate three forms of variances. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5355

24 Samples

Download data: GPR