GEO DataSets

Analysis of protein kinase B alpha (PKBalpha) knockout embryonic fibroblasts (MEFs) following treatment with a standard induction cocktail to induce differentiation into adipocytes. Cells examined up to 48 hours following treatment. Results provide insight into the role of PKBalpha in adipogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 5 time sets

Platform:

GPL339

Series:

GSE2746

36 Samples

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(Submitter supplied) Mouse embryonic fibroblasts (MEFs) were generated from 13.5-day-old embryos obtained from heterozygous PKBa mice intercrosses (Yang et al., 2003). Briefly, after dissection of head and visceral organs for genotyping, embryos were minced and trypsinized for 30 min at 37°C. Embryonic fibroblasts were then plated and maintained in Dulbecco’s Modified Eagle Medium (DMEM) with 10% foetal calf serum (FCS) (Life Technologies), 100 units/ml of penicillin and 100 mg/ml of streptomycin at 37°C in an atmosphere of 5% CO2. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1784

Platform:

GPL339

36 Samples

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(Submitter supplied) The differentiation of preadipocytes into adipocytes is controlled by several transcription factors, including peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα), which are known as master regulators of adipogenesis. BCL11B is a zinc finger-type transcription factor that regulates the development of the skin and central nervous and immune systems. Here, we found that BCL11B was expressed in the white adipose tissue (WAT), particularly the subcutaneous WAT and that BCL11B−/− mice had a reduced amount of subcutaneous WAT. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

2 Samples

Download data: CEL

(Submitter supplied) We used an unbiased systems biology approach to study the regulation of gene expression in human adipose tissue focusing on inflammation. We show that microRNAs play a major role as regulators of CCL2 production in obesity.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

36 Samples

Download data: CEL

(Submitter supplied) We used an unbiased systems biology approach to study the regulation of gene expression in human adipose tissue focusing on inflammation. We show that microRNAs play a major role as regulators of CCL2 production in obesity.

Organism:

Homo sapiens; synthetic construct

Type:

Non-coding RNA profiling by array

Platform:

GPL8786

56 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens; synthetic construct

Type:

Methylation profiling by array; Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL6244 GPL8786

148 Samples

Download data: CEL

(Submitter supplied) We used an unbiased systems biology approach to study the regulation of gene expression in human adipose tissue focusing on inflammation. We show that microRNAs play a major role as regulators of CCL2 production in obesity.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

56 Samples

Download data: CEL

(Submitter supplied) We have shown that C57BL/6J CCR5 knockout mice develop 30.4% ± 8.6% fewer B16 F10 lung nodules compared to wild type mice after the intravenous injection of 100,000 B16 F10 cells. We sought to understand this phenomenon by comparing gene expression in the lungs of these mice at 6, 24, and 48 hours after tumor injection.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4840

Platform:

GPL1261

6 Samples

Download data: CEL

Analysis of lungs from CCR5-deficient mutants 6 to 48 hrs after tail vein injection with B16-F10 melanoma cells. Lungs of CCR5-deficient mutants develop fewer metastatic nodules than in wild type animals. Results provide insight into the molecular mechanisms underlying CCR5-dependent metastasis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 3 time sets

Platform:

GPL1261

Series:

GSE51422

6 Samples

Download data: CEL