GEO DataSets

Analysis of monocytes infected with adenovirus expressing c-Myb or its oncogenic variant v-Myb, a mutated (11 AA substitutions) and truncated (N- and C-termini) version of c-Myb. Results identify dramatic, qualitative changes in the transcriptional acitivity of c-Myb caused by oncogenic mutations.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 infection sets

Platform:

GPL570

Series:

GSE2816

6 Samples

Download data: CEL

(Submitter supplied) The transcriptional activities of c-Myb and its oncogenic variant v-Myb were compared by expressing them in primary human monocytes using recombinant adenovirus vectors. All the samples were compared to cells infected with a control adenovirus expressing only GFP. The results showed that v-Myb, which differs from c-Myb only by N- and C-terminal deletions and eleven amino acid substitutions, has a qualitatively different transcriptional activity. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1427

Platform:

GPL570

6 Samples

Download data: CEL

(Submitter supplied) The transcriptional activities of c-Myb and its oncogenic variant v-Myb were compared by expressing them in human MCF7 cells using recombinant adenovirus vectors. A hybrid construct, 3Mutc, which is a variant of c-Myb harboring three v-Myb-derived DNA binding domain mutations was also analyzed. All the samples were compared to cells infected with a control adenovirus. The results showed that v-Myb, which differs from c-Myb only by N- and C-terminal deletions and eleven amino acid substitutions, has a qualitatively different transcriptional activity. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1384

Platform:

GPL96

8 Samples

Download data: CEL

Analysis of transcriptional activity in MCF7 cells infected with control adenovirus versus adenovirus expressing c-Myb, oncogenic variant v-Myb, or a 3MutC hybrid construct. Results indicate that v-Myb is a distinct transcriptional regulator with a unique set of activities.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 agent sets

Platform:

GPL96

Series:

GSE2815

8 Samples

Download data: CEL

(Submitter supplied) Recombinant adenovirus vectors were used to express wild type or domain swap mutants of A-Myb and c-Myb transcription factors in MCF-7 cells or pimary lung epithelial cells or fibroblasts. The results show that Myb proteins have extreme context specificity and identify sub-domains responsible for the activation of specific sets of target genes. Keywords = Myb proteins Keywords = oncogenes Keywords = transcription Keywords = gene activation Keywords: ordered

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL96 GPL8300

25 Samples

Download data: CEL