GEO DataSets

Cells arrested in G1 for CLN3 overexpression. Included as part of a comprehensive identification of cell cycle-regulated genes.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 2 time sets

Platform:

GPL61

Series:

GSE25

2 Samples

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(Submitter supplied) Yeast cells were arrested either in G1 (for CLN3 overexpression) or in G2/M (for CLB2 overexpression). The cyclin was then induced, and samples were taken. This study is described in more detail in Spellman PT et al.(1998) Mol Biol Cell 9:3273-97 Keywords: other

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS110

Platform:

GPL61

3 Samples

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(Submitter supplied) Small G1 daughter yeast cells were isolated by centrifugal elutriation. They were then released into YEP ethanol, and followed through one cell cycle, with samples being taken every 30 minutes. This study is described in more detail in Spellman PT et al.(1998) Mol Biol Cell 9:3273-97 Keywords: time-course

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS39

Platform:

GPL59

14 Samples

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(Submitter supplied) Yeast cells were blocked in telophase using a cdc15-2 temperature senstive mutant at restrictive temperature. The culture was then shifted to permissive temperature (25oC), and released into the cell cycle. Samples were then taken during the course of almost three full cell cycles. This study is described in more detail in Spellman PT et al.(1998) Mol Biol Cell 9:3273-97 Keywords: time-course

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL62

25 Samples

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(Submitter supplied) These data are from a time series, where yeast were arrested in alpha-factor, then the alpha-factor was washed out, and the cells were release into fresh medium. Samples were taken every 7 minutes as the cells went through the cell cycle. This study is described in more detail in Spellman PT et al.(1998) Mol Biol Cell 9:3273-97 Keywords: time-course

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS38

Platforms:

GPL59 GPL60

18 Samples

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Culture synchronized by elutriation and samples removed at several time points up to 6.5 h. Included as part of a comprehensive identification of cell cycle-regulated genes.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 14 time sets

Platform:

GPL59

Series:

GSE24

14 Samples

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Culture synchronized by alpha factor arrest, then samples taken every 7 minutes as cells went through cell cycle. Included as part of a comprehensive identification of cell cycle-regulated genes.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 16 time sets

Platform:

GPL59

Series:

GSE22

16 Samples

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(Submitter supplied) The wild-type (grown on galactose or glucose) or msn2/4 mutant (grown on galactose) strains were grown aerobically. At time zero (generation 0) the sparge gas was switched from air to O2-free N2 and samples were harvested after 0 (aerobic control), 0.04, 0.08, 0.19, and 2 generations of anaerobic growth. Keywords: time-course

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Datasets:

GDS2002 GDS2003

Platform:

GPL1535

45 Samples

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Analysis of catabolite-derepressed (galactose) wildtype JM43 and isogenic msn2/4 mutant KKY8 cells shifted to short-term anaerobiosis (2 generations). Msn2 and 4 are key stress factors. Results suggest Msn2/4 involvement in metabolic remodeling during acclimatization to short-term anaerobiosis.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log2 ratio, 2 genotype/variation, 2 protocol, 5 time sets

Platform:

GPL1535

Series:

GSE1879

30 Samples

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Analysis of catabolite-repressed (glucose) or derepressed (galactose) wildtype JM43 cells shifted from aerobiosis to anaerobiosis (2 generations). Results identify metabolic remodeling that occurs during acclimatization to short-term anaerobiosis in galactose but not in glucose.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log2 ratio, 2 growth protocol, 2 protocol, 5 time sets

Platform:

GPL1535

Series:

GSE1879

30 Samples

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(Submitter supplied) DNA topoisomerase-2 and high mobility group protein Hmo1 are known to regulate chromatin architecture by regulating gene boundaries. Here we report how these proteins affect global RNA level after inactivation of Top2 and Hmo1. Our data indicate that inactivating Hmo1 has a drastic effect on transcription levels of 20% yeast genes, however, this phenomenon can slightly be rescued by inactivating Top2 functions. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Other

Platforms:

GPL17143 GPL18249

18 Samples

Download data: BED, BEDGRAPH, TXT

(Submitter supplied) DNA topoisomerases assist DNA replication & transcription events by controlling supercoiling alterations. We investigated supercoil distribution across the yeast genome and compared with the accumulation of RNA pol2 and DNA topoisomerases particularly in S-phase. Our data indicate that Top2 along with Hmo1 maintain negative supercoil at gene boundaries by stabilizing alternative DNA structures. To understand how DNA superhelical tension accumulates across the genome we have adopted previously described method [Naughton C et al., 2013] to budding yeast where a biotin molecule was attached to TMP via a linker (bTMP). more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL7250

68 Samples

Download data: BED, BEDGRAPH, CEL