GEO DataSets

(Submitter supplied) The CDMC_Drosophila_7K2 array is a primarily cDNA-based Drosophila melanogaster glass microarray. The array features 5,756 Berkeley Drosophila Genome Project cDNAs, 1,078 NIH Testis cDNAs (Andrews et al. 2000) and 546 gene fragments that were amplified from genomic DNA, together representing approximately 50% of the predicted genes in D. melanogaster. The array is produced by the Canadian Drosophila Microarray Centre at the University of Toronto and is available for distribution to academic labs. more...

Organism:

Drosophila melanogaster

1 DataSet

5 Series

19 Samples

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(Submitter supplied) The expression profiles of Drosophila Kc167 and SL2 cells were compared on the CDMC_Drosophila_7k2 array platform. Three independent RNA samples from each cell line were isolated and compared in a pairwise fashion. Dye-flip experiments were also performed. See Neal et al. 2003 for results and discussion. Keywords = Kc167 Keywords = SL2 Keywords = Drosophila Keywords: other

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL311

6 Samples

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(Submitter supplied) A large batch of Drosophila Kc167 total RNA was prepared and was used for 3 homotypic hybridizations in order to evaluate the inter-slide variability using the CDMC_Drosophila_7k2 platform. See Neal et al. 2003 for results and discussion. Keywords = Kc167 Keywords = Drosophila Keywords = Platform Variability Keywords: other

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL311

3 Samples

Download data: TIFF

(Submitter supplied) Three different amounts, 20 ug (GSM6155), 40 ug (GSM6157) and 80ug (GSM6158), of total Kc167 RNA were used in homotypic labelings to determine the optimal amount of RNA necesary to produce the most useable data using the CDMC_Drosophila_7k2 array. See Neal et al. 2003 for results and discussion. Keywords = Kc167 Keywords = Drosophila Keywords = RNA amount Keywords: other

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS374

Platform:

GPL311

3 Samples

Download data: TIFF

Analysis of the effect of varying starting quantity of RNA. Three different amounts, 20 ug, 40 ug and 80 ug total Kc167 RNA used in homotypic labelings to determine optimal quantity of RNA.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, log ratio, 3 protocol sets

Platform:

GPL311

Series:

GSE417

3 Samples

Download data: TIFF

(Submitter supplied) Time course analysis series in Development of the transcriptome from Drosophila melanogaster using the Heidelberg FlyArray. All stages were hybridized against embryonic stage 0-4 h as reference control. Keywords: time-course

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL517

24 Samples

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(Submitter supplied) Identification and annotation of all the genes in the sequenced Drosophila genome is a work in progress. Wild-type testis function requires many genes and is thus of potentially high value for the identification of transcription units. We therefore undertook a survey of the repertoire of genes expressed in the Drosophila testis by computational and microarray analysis. We generated 3141 high-quality testis expressed sequence tags (ESTs). more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL5

8 Samples

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(Submitter supplied) The array was assembled using 1,681 testes ESTs, representing 1,527 Drosophila melanogaster EST sequences and 144 anonymous clones from the bs collection (CLONE_ID). An aliquot of the same DNA preparation used in the sequencing reactions (GB_ACC), diluted 2/50 in TE, provided material for the PCRs. Inserts were PCR amplified (1X PCR buffer [Life Technologies], 1.5 mM MgCl2, 0.2 mM dNTPs, 1 µM T7 22-mer primer, 1 µM T3 20-mer primer, 2 µl of plasmid DNA dilution [approximately 10 ng], 0.035 U/µl recombinant Taq DNA polymerase [Life Technologies], in a volume of 100 µl, cycled 94C 2 min, 30X[94 C 0.5 min, 55 C 0.5 min, 2.5 min 72 C], 10 min 72 C in a PTC-225 DNA Engine Tetrad [MJ Research]), and successful amplification was confirmed by agarose gel electrophoresis (PCR-QC: passed = product present, failed = no product). more...

Organism:

Drosophila melanogaster

1 Series

8 Samples

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(Submitter supplied) The in-house microarray consists of 167 RT-PCR derived DNA fragments representing 167 ORFs from bovine. The DNA fragments are printed 4 times on the surface of the microarray (four-replicate blocks). The 167 immune and endocrine genes grouped into the following categories: cytokine genes, chemoattractants or chemokines and their respective receptor genes, immune regulatory genes, T-cell associated genes, pattern recognition or innate host defense genes, immunglobin (Ig) or Ig-related genes, complement genes, antigen processing or presenting genes, and genes encoding endocrine hormones or their receptors. more...

Organism:

Arabidopsis thaliana; Bos taurus

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(Submitter supplied) Comparison of gene expression in wild type, Oregon[R], Antennapedia[73b], Apterous[56f] Drosophila melanogaster strains. Keywords: other

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL20

36 Samples

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(Submitter supplied) http://www.nature.com/ng/journal/v33/n2/abs/ng1086.html. Little is known about broad patterns of variation and evolution of gene expression during any developmental process. Here we investigate variation in genome-wide gene expression among Drosophila simulans, Drosophila yakuba and four strains of Drosophila melanogaster during a major developmental transition?the start of metamorphosis. Differences in gene activity between these lineages follow a phylogenetic pattern, and 27% of all of the genes in these genomes differ in their developmental gene expression between at least two strains or species. more...

Organism:

Drosophila simulans; Drosophila yakuba; Drosophila melanogaster

Type:

Expression profiling by array

5 related Platforms

48 Samples

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(Submitter supplied) Little is known about broad patterns of variation and evolution of gene expression during any developmental process. Here we investigate variation in genome-wide gene expression among Drosophila simulans, Drosophila yakuba and four strains of Drosophila melanogaster during a major developmental transition-the start of metamorphosis. Differences in gene activity between these lineages follow a phylogenetic pattern, and 27% of all of the genes in these genomes differ in their developmental gene expression between at least two strains or species. more...

Organism:

Drosophila simulans; Drosophila yakuba; Drosophila melanogaster

Type:

Expression profiling by array

Platforms:

GPL227 GPL228

26 Samples

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(Submitter supplied) Drosophila simulans blue gut larvae vs. white prepupae Keywords: repeat sample

Organism:

Drosophila simulans; Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL228

4 Samples

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(Submitter supplied) Drosophila melanogaster Samarkand blue gut larvae vs. white prepupae Keywords: repeat sample

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL228

6 Samples

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(Submitter supplied) Drosophila melanogaster OregonR blue gut larvae vs. white prepupae Keywords: repeat sample

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL228

4 Samples

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(Submitter supplied) Drosophila melanogaster Netherlands2 blue gut larvae vs. white prepupae Keywords: repeat sample

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL228

4 Samples

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(Submitter supplied) Drosophila melanogaster CantonS blue gut larvae vs. white prepupae Keywords: repeat sample

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL228

4 Samples

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(Submitter supplied) Drosophila yakuba white prepupae vs. blue gut larvae Keywords: repeat sample

Organism:

Drosophila melanogaster; Drosophila yakuba

Type:

Expression profiling by array

Platform:

GPL227

4 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL208

6 Samples

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(Submitter supplied) Replicate hybridizations using mouse spleen mRNA Keywords: repeat sample

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL208

3 Samples

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