GEO DataSets

(Submitter supplied) Streptococcus pyogenes is an obligate human pathobiont associated with many disease states. Here, we present a novel model of S. pyogenes infection using intact murine epithelium. From this model, we were able to perform RNA sequencing to evaluate the genetic changes undertaken by both the bacterium and host at 5 and 24 hours post infection. Analysis of these genomic data demonstrate that S. pyogenes undergoes significant genetic adaptation to successfully infect the murine epithelium, including changes to metabolism and activation of the Rgg2/Rgg3 quorum sensing (QS) system. more...

Organism:

Mus musculus; Streptococcus pyogenes NZ131

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL33562 GPL33561 GPL13112

11 Samples

Download data: CSV, XLSX

(Submitter supplied) RNAseq analysis was performed for C. trachomatis serovar L2 and S. pyogenes NZ131 in the presence and absence of tryptophan to monitor transcriptional changes.

Organism:

Streptococcus pyogenes NZ131; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18573 GPL30099 GPL30892

48 Samples

Download data: XLSX

(Submitter supplied) The speB gene of Streptococcus pyogenes has a long 5' UTR with unknown functions. Preliminary studies showed that the partial mutation of this region led to altered expression patterns of the speB gene and ropB, its upstream gene. In this study, we used microarray to examine the effect of speB 5' UTR mutation on the S. pyogenes transcriptome.

Organism:

Streptococcus pyogenes NZ131; Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL11420

12 Samples

Download data: CEL, CHP

(Submitter supplied) Differences in gene expression between the wild-typ SF370 and its rgg2 mutant was identified with samples collected from mid-exponential and post-exponential phases of growth

Organism:

Streptococcus pyogenes NZ131; Streptococcus pyogenes M1 GAS

Type:

Expression profiling by array

Platform:

GPL11420

12 Samples

Download data

(Submitter supplied) Streptococcus pyogenes (Group A streptococcus, GAS) is an important human pathogen that causes a variety of infectious diseases and sequelae. Recent studies showed virulence factor expression was controlled at multiple levels, including the post-transcriptional regulation. In this study, we examined the global half-lives of S. pyogenes mRNAs and explored the role RNase Y played in mRNA metabolism with microarray analysis. more...

Organism:

Streptococcus pyogenes NZ131; Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL11420

20 Samples

Download data: CEL

(Submitter supplied) Whole genome expression profile comparing MGAS315 treated with XIP pheromone versus vehicle-treated cells Interpretations are described further in the manuscript to be submitted: authors Mashburn-Warren, Morrison, and Federle. Title: The Cryptic Competence Pathway in Streptococcus pyogenes is Controlled by a Peptide Pheromone.

Organism:

Streptococcus pyogenes MGAS315; Streptococcus pyogenes NZ131

Type:

Expression profiling by genome tiling array

Platform:

GPL15576

2 Samples

Download data: CALLS, PAIR, TXT

(Submitter supplied) Small non-coding RNAs (sRNAs) have attracted attention as a new class of gene regulators in eukaryotes and in bacteria. In this study, we identified novel sRNAs of the human pathogen Streptococcus pyogenes M49 (GAS M49). A temporal expression profile of potential sRNAs was determined for (GAS M49). Cells were grown in chemical defined medium (CDM), Todd-Hewitt broth (Invitrogen) supplemented with 0.5% yeast extract (THY) or Brain-Heart-Infusion (BHI) complex medium using tiling arrays representing the intergenic regions of the GAS M49 genome. more...

Organism:

Streptococcus pyogenes NZ131; Streptococcus pyogenes M49 591

Type:

Non-coding RNA profiling by genome tiling array

Platform:

GPL14116

16 Samples

Download data: TXT

(Submitter supplied) GAS is a highly virulent Gram-positive bacterium. For the successful infection GAS express many virulence factors, which are clustered together with transcriptional regulators in distinct genomic regions. Ralp3 is a central regulator of the ERES region. In this study, we investigated the role of Ralp3 in GAS pathogenesis. To characterize the Ralp3 regulatory function on the whole genome level, GAS M49 wild type and Äralp3 mutant strains were comprehensively compared by two colour microarray analysis. more...

Organism:

Streptococcus pyogenes M49 591; Streptococcus pyogenes NZ131

Type:

Expression profiling by array

Platform:

GPL13816

16 Samples

Download data: TXT

(Submitter supplied) Streptococcus pyogenes (group A Streptococcus, GAS) responds to environmental changes in a manner that results in an adaptive regulation of the transcriptome. Global transcriptional regulators are able to integrate important extracellular and intracellular information and are responsible for modulation of the transcriptional network. The roles of several global transcriptional regulators in adaptation and virulence gene expression have been described. more...

Organism:

Streptococcus pyogenes NZ131

Type:

Expression profiling by array

Platform:

GPL11420

40 Samples

Download data: CEL

(Submitter supplied) Whole genone expression profile comparing wild-type NZ131 to serR deletion mutant, grown in C-medium Mutants and interpretation are described further in the manuscript to be submitted: LaSarre and Federle, 2010. Title: Regulation and Consequence of Serine Catabolism in Streptococcus pyogenes.

Organism:

Streptococcus pyogenes NZ131

Type:

Expression profiling by genome tiling array

Platform:

GPL15576

2 Samples

Download data: CALLS, PAIR, TXT

(Submitter supplied) Expression array including select intergenic region tiles of S. pyogenes NZ131 Protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. See manufacturer's website at http://www.nimblegen.com/.

Organism:

Streptococcus pyogenes NZ131

2 Series

1 Related Platform

4 Samples

Download data: NDF, NGD

(Submitter supplied) in situ oligonucleotides, custom-made tiling array of the intergenic regions (50 nucleotides probes length with a 15 nucleotides overlap) for both strands. Synthesis performed by Geniom Microarray Facility (Febit biomed GmbH, Heidelberg, Germany)

Organism:

Streptococcus pyogenes NZ131

1 Series

16 Samples

Download data

(Submitter supplied) Custom Array GE 8x15K; Agilent [025553_D_GEO_20090929.txt] Protocol: see manufacturer's web site at http://www.agilent.com/

Organism:

Streptococcus pyogenes NZ131

1 Series

1 Related Platform

16 Samples

Download data

(Submitter supplied) Custom Array GE 8x15K; Agilent [025553_D_GEO_20090929.txt] Arrays of this design have barcodes that begin with 16025553 or 2525553. Orientation: Features are numbered numbered Left-to-Right, Top-to-Bottom as scanned by an Agilent scanner (barcode on the left, DNA on the back surface, scanned through the glass), matching the FeatureNum output from Agilent's Feature Extraction software. The ID column represents the Agilent Feature Extraction feature number. more...

Organism:

Streptococcus pyogenes NZ131

1 Related Platform

Download data

(Submitter supplied) Standard Affymetrix procedures

Organism:

Streptococcus pyogenes NZ131

4 Series

84 Samples

Download data: CDF

(Submitter supplied) Expression array including select intergenic region tiles of S. pyogenes NZ131 Protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. See manufacturer's website at http://www.nimblegen.com/.

Organism:

Streptococcus pyogenes NZ131

1 Related Platform

Download data: NDF, NGD