GEO DataSets

(Submitter supplied) Replication-deficient adenovirus (Ad) type 5 capsids induce potent host innate immune responses. One system pivotal in host innate immune defense is the complement system. Intriguingly, excessive or inappropriate complement activation can result in extreme tissue damage and systemic inflammatory responses similar to those noted immediately after high dose systemic Ad vector injections. To determine if the complement system has a significant role in intensifying Ad-associated acute toxicities, we compared complement deficient (C3-knockout (KO)) mice responses to those of wild type (WT) mice following systemic challenge with Ad vectors. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1826

Platform:

GPL3222

29 Samples

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Analysis of livers from complement-deficient C3 knockout mutants injected with a first generation LacZ adenovirus (Ad) vector. Ad vectors are used in gene delivery. Results provide insight into the role of the complement system in elaborating multiple Ad-associated acute inflammatory responses.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 2 genotype/variation, 2 infection sets

Platform:

GPL3222

Series:

GSE4128

29 Samples

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(Submitter supplied) Protocol for Preparing Poly-Lysine Slides for Microarrays page 1 Basics: -The following protocol is for coating 180 slides with poly-l-lysine. -Be sure slide racks are bent slightly inwards in the middle to hold slides more securely. -DO NOT use powdered gloves at any time during this protocol. -To avoid dust, try to keep slides covered or submerged in solution at all times. -It is recommended that at least two batches of poly-lysine slides are prepared per printing. more...

Organism:

Mus musculus

1 DataSet

4 Series

86 Samples

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