GEO DataSets

(Submitter supplied) The microarrays were subjected to two-color hybridization using Cy5 and Cy3 dyes incorporated in the cDNAs synthesized from total RNA samples of strains. We compared each gene’s transcription level between the wild-type (WT) strain 13 (labeled by Cy5) and the virX-mutant strain (TS186) (labeled by Cy3) on a single DNA microarray.

Organism:

Clostridium perfringens str. 13

Type:

Expression profiling by array

Platform:

GPL7332

4 Samples

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(Submitter supplied) 2723 genes and 144 intergenic regions of strain 13 were amplified by PCR with gene-specific primers and PCR products were confirmed by gel electrophoresis In total, 2867 probes were spotted onto poly-L-Lysine coated glass slides in twice (SD10011; Matsunami Glass Ind., Osaka, Japan) using SPBIO (Hitachi Software Engineering, Tokyo, Japan).

Organism:

Clostridium perfringens str. 13

2 Series

10 Samples

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