GEO DataSets

(Submitter supplied) To elucidate transcriptional differences between naïve, tolerant and memory CD8+ T cells, genome-wide gene expression profiling was performed. The indicated cell populations were purified from immunized or naïve Rag-/- TCR mice by cell sorting and RNA was extracted labeled and hybridized to an Affymetrix custom mouse array GNF1M GeneChip. RNA from sorted samples was prepared, split into two aliquots prior to making labeled cDNA, so that two arrays were used for each cell population.Alterations in transcript levels were determined through pair wise comparisons of naïve cells to either memory or tolerant CD8+ T cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2327

Platform:

GPL1073

6 Samples

Download data: CEL

Analysis of tolerant, naïve, and memory CD8+ T cells purified from TCRxGag transgenics. T cell tolerance to self-proteins prevents autoimmunity but impedes the response to tumor-associated antigens. Results provide insight into the feasibility of rescuing tolerant cells for tumor immunotherapy.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 cell type sets

Platform:

GPL1073

Series:

GSE3800

6 Samples

Download data: CEL

(Submitter supplied) GNF1M We identified a non-redundant set of target sequences for the mouse using the following sources: RefSeq (12,029 sequences), Celera (29,331 sequences), and RIKEN (46,299 sequences). First, all sequences were RepeatMasked to remove repetitive elements. Next, sequence identity between individual sequences was established using pairwise BLAT or BLAST and sim4. The results from single-linkage clustering were further triaged to produce a final target set of 36,182 targets with the highest degree of confidence of computational prediction (biasing toward sequences containing Interpro domains and away from non-coding RNAs). more...

Organism:

Mus musculus

2 DataSets

9 Series

310 Samples

Download data: CDF, CIF, GIN, PROBE, PSI, SIF, TAB