GEO DataSets

(Submitter supplied) Eukaryotic cells use numerous mechanisms to ensure that no segment of their DNA is re-replicated within a single cell cycle. Despite longstanding speculation that such tight regulation is needed to protect cells from genomic alterations, this notion has never been experimentally tested. Here we show that even just transient and limited re-replication in Saccharomyces cerevisiae can strongly induce the critical first step of gene amplification, increasing gene copy number from one to two or more. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome variation profiling by genome tiling array

Platform:

GPL3412

203 Samples

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(Submitter supplied) PCR products representing ORF and intergenic yeast genomic sequences were amplified. Products were spotted on poly-L-lysine coated glass slides using a linear servo arrayer from the DeRisi Lab at UCSF. Blast analysis of oligos sequences was used to determine the position of the final PCR product. Those oligo pairs which Blast analysis indicated could not generate a product or generated more than one product given the flag –1. more...

Organism:

Saccharomyces cerevisiae

6 Series

1083 Samples

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