GSM4783998[Accession] - GEO DataSets

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Items: 4

Select item 2001580041.

HyPR-seq: Single-cell quantification of chosen RNAs via hybridization and sequencing of DNA probes

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens; Mus musculus

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL21626 GPL21697 GPL24247
79 Samples

Download data: H5, TXT

Series

Accession:: GSE158004

ID:: 200158004

PubMed Full text in PMC Similar studies

Select item 2001580032.

HyPR-seq: Single-cell quantification of chosen RNAs via hybridization and sequencing of DNA probes (HyPR-seq dataset)

(Submitter supplied) Single-cell quantification of RNAs is important for understanding cellular heterogeneity and gene regulation, yet current approaches suffer from low sensitivity for individual transcripts, limiting their utility for many applications. Here we present HyPR-seq, a method to sensitively quantify the expression of 10 to 100 chosen genes in single cells. HyPR-seq involves hybridizing DNA probes to RNA, distributing cells into nanoliter droplets, amplifying the probes with PCR, and sequencing the amplicons to quantify the expression of chosen genes. more...