GSM4626512[Accession] - GEO DataSets

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Items: 3

Select item 2001527851.

Immunological fingerprint of 4CMenB recombinant antigens via protein microarray

(Submitter supplied) In this study, we describe the development and use of an ad hoc protein microarray to study the immune response induced by the three major 4CMenB antigenic components (fHbp, NHBA and NadA) in individual sera from vaccinated infants, adolescents and adults.

Organism:: Homo sapiens; Neisseria meningitidis

Type:: Protein profiling by protein array

Platform:: GPL28712
219 Samples

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Accession:: GSE152785

ID:: 200152785

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Select item 1000287122.

4CMenB antigenic components protein microarray

(Submitter supplied) Purified recombinant protein were diluted at final concentration of 0.5 mg/ul in 40% glycerol and transferred into 384-well microtiterplates. One droplet of 0.2 nL protein solution was deposited per spot on nitrocellulose-coated slides (FAST slides, Schleicher and Schuell) using the non-contact printer Marathon Spotter (Arrayjet, Edinburgh, UK). Printing was performed in a cabinet with controlled humidity and temperature (55-60% and 12 °C respectively), resulting in spots of 90-110 μm in diameter range. more...