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Items: 3

1.

Recognition of cellular RNAs by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids

(Submitter supplied) We showed that the majority of the signal observed in S9.6 immunofluorescence microscopy images of fixed human cells arises from RNA, not RNA:DNA hybrids. S9.6 staining is quantitatively unchanged by pre-treatment with the human RNA:DNA hybrid-specific nuclease, RNase H1, despite experimental verification that the enzyme was active in situ. S9.6 staining was, however, significantly sensitive to pre-treatments with RNase T1, and in some cases RNase III, two ribonucleases that specifically degrade single-stranded and double-stranded RNA, respectively. more...
Organism:
Homo sapiens
Type:
Other
Platforms:
GPL20301 GPL21290
18 Samples
Download data: BW
Series
Accession:
GSE141833
ID:
200141833

PubMed Full text in PMC Similar studies SRA Run Selector

2.

Illumina HiSeq 3000 (Homo sapiens)

Organism:
Homo sapiens
1138 Series
28241 Samples
Download data
Platform
Accession:
GPL21290
ID:
100021290

3.

RNase T1 (DG3G)

Organism:
Homo sapiens
Source name:
NTREA-2 D1
Platform:
GPL21290
Series:
GSE141833
Download data: BW
Sample
Accession:
GSM4213949
ID:
304213949

SRA Run Selector

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db=gds|term=GSM4213949[Accession]|query=1|qty=2|blobid=MCID_674c4f4a96d9ad040624f99e|ismultiple=true|min_list=5|max_list=20|def_tree=20|def_list=|def_view=|url=/Taxonomy/backend/subset.cgi?|trace_url=/stat?
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