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Items: 3

1.

CRISPR-mediated mutagenesis of RNA editing cis-regulatory elements

(Submitter supplied) We used CRISPR KI technology to mutagenize the cis-regulatory elements including the editing stem and the editing complementary sequence of three RNA editing substrates-NEIL1, TTYH2, AJUBA. We designed single nucleotide variants, double-nucleotide variants and other mutant isoforms to interrogate the primary sequence and the secondary structures around the endogenous RNA editing sites. Gene specific primers were used to make the NGS library for each locus. more...
Organism:
Homo sapiens; synthetic construct
Type:
Other
Platforms:
GPL27609 GPL21697
25 Samples
Download data: TXT
Series
Accession:
GSE138860
ID:
200138860
2.

NextSeq 550 (Homo sapiens)

Platform
Accession:
GPL21697
ID:
100021697
3.

cDNA of NEIL1 mutants from double strand donor knockin rep2

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120525
ID:
304120525
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Supplemental Content

db=gds|term=GSM4120525[Accession]|query=1|qty=3|blobid=MCID_6748e0bb43705129cf4bba64|ismultiple=true|min_list=5|max_list=20|def_tree=20|def_list=|def_view=|url=/Taxonomy/backend/subset.cgi?|trace_url=/stat?
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