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Items: 4

1.

Differential gene expression profiles are dependent upon method of peripheral blood RNA isolation

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL570
48 Samples
Download data: CEL
Series
Accession:
GSE12711
ID:
200012711
2.

Differential gene expression profiles are dependent upon method of peripheral blood RNA isolation (PHA)

(Submitter supplied) RNA isolation and purification steps greatly influence the results of gene expression profiling. There are two commercially available products for whole blood RNA collection, PAXgene and Tempus blood collection tubes, and each comes with their own RNA purification method. We examined the impact of RNA isolation methods on gene expression profiles. We demonstrated that peripheral blood RNA isoaltion can critically impact differential expression results, particularly in the clinical setting where fold-change differences are typically small and there is inherent variability within biological corhorts Keywords: micorarray quality assurance, phytohemagglutinin (PHA)
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL570
28 Samples
Download data: CEL
Series
Accession:
GSE12709
ID:
200012709
3.

[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

(Submitter supplied) Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://www.ncbi.nlm.nih.gov/projects/geo/info/geo_affy.html June 03, 2009: annotation table updated with netaffx build 28 June 06, 2012: annotation table updated with netaffx build 32 June 23, 2016: annotation table updated with netaffx build 35 Protocol: see manufacturer's web site Complete coverage of the Human Genome U133 Set plus 6,500 additional genes for analysis of over 47,000 transcripts All probe sets represented on the GeneChip Human Genome U133 Set are identically replicated on the GeneChip Human Genome U133 Plus 2.0 Array. more...
Organism:
Homo sapiens
602 DataSets
5670 Series
61 Related Platforms
175576 Samples
Download data
Platform
Accession:
GPL570
ID:
100000570
4.

PHA stimulated, Tempus, replicate Y (Blood_PHA25_ABI_Y)

Organism:
Homo sapiens
Source name:
Peripheral blood
Platform:
GPL570
Series:
GSE12709 GSE12711
Download data: CEL
Sample
Accession:
GSM318887
ID:
300318887
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