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1.

Design and testing of genome-proxy microarrays to profile marine microbial communities

(Submitter supplied) Microarrays are useful tools for detecting and quantifying specific functional and phylogenetic genes in natural microbial communities. In order to track uncultivated microbial genotypes and their close relatives in an environmental context, we designed and implemented a “genome proxy” microarray that targets microbial genome fragments recovered directly from the environment. Fragments consisted of sequenced clones from large-insert genomic libraries from microbial communities in Monterey Bay, the Hawaii Ocean Time-series station ALOHA, and Antarctic coastal waters. more...
Organism:
uncultured crenarchaeote 4B7; Prochlorococcus marinus subsp. pastoris str. CCMP1986; Prochlorococcus marinus str. MIT 9312; uncultured marine gamma proteobacterium EBAC31A08; uncultured marine bacterium EB0_41B09; uncultured proteobacterium 60D04; Prochlorococcus marinus str. MIT 9313; Prochlorococcus marinus str. MIT 9515; uncultured marine bacterium 577; uncultured gamma proteobacterium eBACHOT4E07; uncultured marine group II euryarchaeote 37F11; uncultured marine bacterium 440; uncultured marine bacterium 583; marine metagenome; uncultured marine alpha proteobacterium; uncultured Pseudomonadota bacterium; uncultured crenarchaeote 74A4; uncultured proteobacterium 65D09; uncultured marine group II euryarchaeote EF100_57A08; uncultured marine bacterium EB000_55B11
Type:
Other
Platform:
GPL6012
28 Samples
Download data: GPR
Series
Accession:
GSE9384
ID:
200009384
2.

A genome proxy array for profiling marine micobial communities

(Submitter supplied) ***Design Overview*** The prototype microarray targeted thirteen BAC or fosmid genome fragments (20-160kb) from both bacteria and archaea (Table 1), recovered from a variety of marine habitats (Table 2), as well as the cyanobacterium Prochlorococcus MED4. These clones were originally sequenced because of the presence of taxonomic marker or specific functional genes. This array consisted of sets of 70-bp oligonucleotides targeting each genome or genome fragment (Fig. more...
Organism:
uncultured crenarchaeote 4B7; Prochlorococcus marinus subsp. pastoris str. CCMP1986; uncultured marine alpha proteobacterium; uncultured marine gamma proteobacterium EBAC31A08; uncultured marine group II euryarchaeote 37F11; uncultured Pseudomonadota bacterium; uncultured crenarchaeote 74A4; uncultured marine bacterium 440; uncultured marine bacterium 577; uncultured marine bacterium 583; uncultured gamma proteobacterium eBACHOT4E07; uncultured marine bacterium EB0_41B09; uncultured proteobacterium 60D04; uncultured proteobacterium 65D09; uncultured marine group II euryarchaeote EF100_57A08; uncultured marine bacterium EB000_55B11
1 Series
28 Samples
Download data
Platform
Accession:
GPL6012
ID:
100006012
3.

Environmental DNA + Prochloro Mixed, 9312 dominant

Organism:
Prochlorococcus marinus subsp. pastoris str. CCMP1986; Prochlorococcus marinus str. MIT 9312; Prochlorococcus marinus str. MIT 9515; Prochlorococcus marinus str. MIT 9313; marine metagenome
Source name:
Environmental DNA with added Prochlorococcus strains, mixed: 10^5 cell/ml 9312, and 10^3 cells/ml each strains MED4, 9515 and 9313
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238789
ID:
300238789
4.

Environmental DNA + Prochloro Mixed, 9515 dominant

Organism:
Prochlorococcus marinus subsp. pastoris str. CCMP1986; Prochlorococcus marinus str. MIT 9312; Prochlorococcus marinus str. MIT 9515; marine metagenome; Prochlorococcus marinus str. MIT 9313
Source name:
Environmental DNA with added Prochlorococcus strains, mixed: 10^5 cell/ml 9515, and 10^3 cells/ml each strains MED4, 9312 and 9313
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238788
ID:
300238788
5.

Environmental DNA + Prochloro Mixed, MED4 dominant

Organism:
Prochlorococcus marinus str. MIT 9313; Prochlorococcus marinus str. MIT 9515; Prochlorococcus marinus subsp. pastoris str. CCMP1986; Prochlorococcus marinus str. MIT 9312; marine metagenome
Source name:
Environmental DNA with added Prochlorococcus strains, mixed: 10^5 cell/ml MED4, and 10^3 cells/ml each strains 9515, 9312 and 9313
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238762
ID:
300238762
6.

Environmental DNA + Prochloro Mixed, all strains equal

Organism:
Prochlorococcus marinus subsp. pastoris str. CCMP1986; marine metagenome; Prochlorococcus marinus str. MIT 9312; Prochlorococcus marinus str. MIT 9313; Prochlorococcus marinus str. MIT 9515
Source name:
Environmental DNA with added Prochlorococcus strains MED4, 9515, 9312, and 9313
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238761
ID:
300238761
7.

Environmental DNA + Prochloro MED4, 10^6 cells/ml

Organism:
marine metagenome; Prochlorococcus marinus subsp. pastoris str. CCMP1986
Source name:
Environmental DNA with added Prochlorococcus strain MED4, 10^6 cells/ml
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238760
ID:
300238760
8.

Environmental DNA + Prochloro MED4, 10^5 cells/ml

Organism:
Prochlorococcus marinus subsp. pastoris str. CCMP1986; marine metagenome
Source name:
Environmental DNA with added Prochlorococcus strain MED4, 10^5 cells/ml
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238759
ID:
300238759
9.

Environmental DNA + Prochloro MED4, 10^4 cells/ml

Organism:
Prochlorococcus marinus subsp. pastoris str. CCMP1986; marine metagenome
Source name:
Environmental DNA with added Prochlorococcus strain MED4, 10^4 cells/ml
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238756
ID:
300238756
10.

Environmental DNA + Prochloro MED4, 10^3 cells/ml

Organism:
Prochlorococcus marinus subsp. pastoris str. CCMP1986; marine metagenome
Source name:
Environmental DNA with added Prochlorococcus strain MED4, 10^3 cells/ml
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238754
ID:
300238754
11.

Environmental DNA + Prochloro MED4, 10^2 cells/ml

Organism:
Prochlorococcus marinus subsp. pastoris str. CCMP1986; marine metagenome
Source name:
Environmental DNA with added Prochlorococcus strain MED4, 10^2 cells/ml
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238753
ID:
300238753
12.

Environmental DNA + Prochloro MED4, 10^1 cells/ml

Organism:
marine metagenome; Prochlorococcus marinus subsp. pastoris str. CCMP1986
Source name:
Environmental DNA with added Prochlorococcus strain MED4, 10^1 cells/ml
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238752
ID:
300238752
13.

Environmental DNA + Prochloro 9515, 10^6 cells/ml

Organism:
marine metagenome; Prochlorococcus marinus str. MIT 9515
Source name:
Environmental DNA with added Prochlorococcus strain 9515, 10^6 cells/ml
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238751
ID:
300238751
14.

Environmental DNA + Prochloro 9515, 10^5 cells/ml

Organism:
marine metagenome; Prochlorococcus marinus str. MIT 9515
Source name:
Environmental DNA with added Prochlorococcus strain 9515, 10^5 cells/ml
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238750
ID:
300238750
15.

Environmental DNA + Prochloro 9515, 10^4 cells/ml

Organism:
marine metagenome; Prochlorococcus marinus str. MIT 9515
Source name:
Environmental DNA with added Prochlorococcus strain 9515, 10^4 cells/ml
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238749
ID:
300238749
16.

Environmental DNA + Prochloro 9515, 10^3 cells/ml

Organism:
marine metagenome; Prochlorococcus marinus str. MIT 9515
Source name:
Environmental DNA with added Prochlorococcus strain 9515, 10^3 cells/ml
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238747
ID:
300238747
17.

Environmental DNA + Prochloro 9515, 10^2 cells/ml

Organism:
Prochlorococcus marinus str. MIT 9515; marine metagenome
Source name:
Environmental DNA with added Prochlorococcus strain 9515, 10^2 cells/ml
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238746
ID:
300238746
18.

Environmental DNA + Prochloro 9515, 10^1 cells/ml

Organism:
Prochlorococcus marinus str. MIT 9515; marine metagenome
Source name:
Environmental DNA with added Prochlorococcus strain 9515, 10^1 cells/ml
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238742
ID:
300238742
19.

Environmental DNA + Prochloro 9313, 10^6 cells/ml

Organism:
Prochlorococcus marinus str. MIT 9313; marine metagenome
Source name:
Environmental DNA with added Prochlorococcus strain 9313, 10^6 cells/ml
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238741
ID:
300238741
20.

Environmental DNA + Prochloro 9313, 10^5 cells/ml

Organism:
Prochlorococcus marinus str. MIT 9313; marine metagenome
Source name:
Environmental DNA with added Prochlorococcus strain 9313, 10^5 cells/ml
Platform:
GPL6012
Series:
GSE9384
Download data: GPR
Sample
Accession:
GSM238740
ID:
300238740
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