GSE80636[Accession] - GEO DataSets - NCBI

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Producing enteroendocrine cells from Lgr5+ intestinal stem cells by manipulating quiescence

(Submitter supplied) Lgr5+ adult intestinal stem cells are highly proliferative throughout life. Single Lgr5+ stem cells can be cultured into 3D epithelial organoids containing all cell types at nearnormal ratios. Culture conditions to generate the main cell types have been established previously, but signals inducing the various types of enteroendocrine cells (EECs) have remained elusive. Here we generate quiescent Lgr5+ stem cells in vitro by inhibition of the EGF-receptor (EGFR) and mitogen-associated protein kinase (MAPK) signaling pathways in organoids, a state that can be readily reversed. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL19057
58 Samples

Download data: CSV

Series

Accession:: GSE80636

ID:: 200080636

PubMed Similar studies SRA Run Selector

Select item 1000190572.

Illumina NextSeq 500 (Mus musculus)

Organism:: Mus musculus

10340 Series
296909 Samples

Platform

Accession:: GPL19057

ID:: 100019057

Select item 3023590203.

Extracted RNA from bulk cells of organoids after 96 hours of Wnt, Notch and Mek inhibition, replicate 3

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359020

ID:: 302359020

SRA Run Selector

Select item 3023590194.

Extracted RNA from bulk cells of organoids after 96 hours of Wnt, Notch and Mek inhibition, replicate 2

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359019

ID:: 302359019

SRA Run Selector

Select item 3023590185.

Extracted RNA from bulk cells of organoids after 96 hours of Wnt, Notch and Mek inhibition, replicate 1

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359018

ID:: 302359018

SRA Run Selector

Select item 3023590176.

Extracted RNA from bulk cells of organoids after 96 hours of Wnt, Notch and EGFR inhibition, replicate 3

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359017

ID:: 302359017

SRA Run Selector

Select item 3023590167.

Extracted RNA from bulk cells of organoids after 96 hours of Wnt, Notch and EGFR inhibition, replicate 2

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359016

ID:: 302359016

SRA Run Selector

Select item 3023590158.

Extracted RNA from bulk cells of organoids after 96 hours of Wnt, Notch and EGFR inhibition, replicate 1

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359015

ID:: 302359015

SRA Run Selector

Select item 3023590149.

Extracted RNA from bulk cells of organoids after 96 hours of Wnt and Notch inhibition, replicate 3

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359014

ID:: 302359014

SRA Run Selector

Select item 30235901310.

Extracted RNA from bulk cells of organoids after 96 hours of Wnt and Notch inhibition, replicate 2

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359013

ID:: 302359013

SRA Run Selector

Select item 30235901211.

Extracted RNA from bulk cells of organoids after 96 hours of Wnt and Notch inhibition, replicate 1

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359012

ID:: 302359012

SRA Run Selector

Select item 30235901112.

Extracted RNA from bulk cells of organoids after 96 hours of Mek inhibition, replicate 3

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359011

ID:: 302359011

SRA Run Selector

Select item 30235901013.

Extracted RNA from bulk cells of organoids after 96 hours of Mek inhibition, replicate 2

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359010

ID:: 302359010

SRA Run Selector

Select item 30235900914.

Extracted RNA from bulk cells of organoids after 96 hours of Mek inhibition, replicate 1

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359009

ID:: 302359009

SRA Run Selector

Select item 30235900815.

Extracted RNA from bulk cells of organoids after 96 hours of EGFR inhibition, replicate 3

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359008

ID:: 302359008

SRA Run Selector

Select item 30235900716.

Extracted RNA from bulk cells of organoids after 96 hours of EGFR inhibition, replicate 2

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359007

ID:: 302359007

SRA Run Selector

Select item 30235900617.

Extracted RNA from bulk cells of organoids after 96 hours of EGFR inhibition, replicate 1

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359006

ID:: 302359006

SRA Run Selector

Select item 30235900518.

Extracted RNA from bulk cells of organoids after 96 hours, replicate 3

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359005

ID:: 302359005

SRA Run Selector

Select item 30235900419.

Extracted RNA from bulk cells of organoids after 96 hours, replicate 2

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359004

ID:: 302359004

SRA Run Selector

Select item 30235900320.

Extracted RNA from bulk cells of organoids after 96 hours, replicate 1

Organism:: Mus musculus

Source name:: Small intestine

Platform:: GPL19057
Series:: GSE80636

Download data: CSV

Sample

Accession:: GSM2359003

ID:: 302359003

SRA Run Selector

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