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Items: 1 to 20 of 28

1.

CRISPR-mediated mutagenesis of RNA editing cis-regulatory elements

(Submitter supplied) We used CRISPR KI technology to mutagenize the cis-regulatory elements including the editing stem and the editing complementary sequence of three RNA editing substrates-NEIL1, TTYH2, AJUBA. We designed single nucleotide variants, double-nucleotide variants and other mutant isoforms to interrogate the primary sequence and the secondary structures around the endogenous RNA editing sites. Gene specific primers were used to make the NGS library for each locus. more...
Organism:
Homo sapiens; synthetic construct
Type:
Other
Platforms:
GPL27609 GPL21697
25 Samples
Download data: TXT
Series
Accession:
GSE138860
ID:
200138860
2.

NextSeq 550 (synthetic construct)

Organism:
synthetic construct
28 Series
593 Samples
Download data
Platform
Accession:
GPL27609
ID:
100027609
3.

NextSeq 550 (Homo sapiens)

Platform
Accession:
GPL21697
ID:
100021697
4.

cDNA of TTYH2 mutants from degenerate donor rep2

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120540
ID:
304120540
5.

cDNA of TTYH2 mutants from degenerate donor rep1

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120539
ID:
304120539
6.

cDNA of NEIL1 mutants from degenerate donor rep2

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120538
ID:
304120538
7.

cDNA of NEIL1 mutants from degenerate donor rep1

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120537
ID:
304120537
8.

cDNA of AJUBA mutants in editng stem rep2

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120536
ID:
304120536
9.

cDNA of AJUBA mutants in editng stem rep1

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120535
ID:
304120535
10.

cDNA of TTYH2 mutants in editing complementary sequence rep2

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120534
ID:
304120534
11.

cDNA of TTYH2 mutants in editing complementary sequence rep1

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120533
ID:
304120533
12.

cDNA of TTYH2 mutants in editing stem rep2

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120532
ID:
304120532
13.

cDNA of TTYH2 mutants in editing stem rep1

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120531
ID:
304120531
14.

cDNA of NEIL1 mutants from Crick strand donor knockin rep2

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120527
ID:
304120527
15.

cDNA of NEIL1 mutants from Crick strand donor knockin rep1

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120526
ID:
304120526
16.

cDNA of NEIL1 mutants from double strand donor knockin rep2

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120525
ID:
304120525
17.

cDNA of NEIL1 mutants from double strand donor knockin rep1

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120524
ID:
304120524
18.

cDNA of NEIL1 mutants from Watson strand donor knockin rep2

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120523
ID:
304120523
19.

cDNA of NEIL1 mutants from Watson strand donor knockin rep1

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120522
ID:
304120522
20.

DNA of NEIL1 mutants from Crick strand donor knockin rep2

Organism:
Homo sapiens
Source name:
HEK293T cells
Platform:
GPL21697
Series:
GSE138860
Download data
Sample
Accession:
GSM4120521
ID:
304120521
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