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Items: 12

1.

PBMC Fixation and Processing for Chromium Single-Cell RNA Sequencing

(Submitter supplied) Background: Interest in single-cell whole transcriptome analysis is growing rapidly, especially for profiling rare or heterogeneous populations of cells. In almost all reported works, investigators have used live cells which represent several inconveniences and limitations. Some recent cell fixation methods did not work with most primary cells including immune cells. Methods: The methanol-fixation and new processing method was introduced to preserve PBMCs for single-cell RNA sequencing (scRNA-Seq) analysis on 10X Chromium platform. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL18573 GPL21290
9 Samples
Download data: MTX, TSV
Series
Accession:
GSE112845
ID:
200112845
2.

Illumina HiSeq 3000 (Homo sapiens)

Platform
Accession:
GPL21290
ID:
100021290
3.

Illumina NextSeq 500 (Homo sapiens)

Platform
Accession:
GPL18573
ID:
100018573
4.

KLM1_methanol_SSC

Organism:
Homo sapiens
Source name:
Pancreatic cancer cell line
Platform:
GPL18573
Series:
GSE112845
Download data: MTX, TSV
Sample
Accession:
GSM3087634
ID:
303087634
5.

KLM1_methanol_PBS

Organism:
Homo sapiens
Source name:
Pancreatic cancer cell line
Platform:
GPL18573
Series:
GSE112845
Download data: MTX, TSV
Sample
Accession:
GSM3087633
ID:
303087633
6.

KLM1_live

Organism:
Homo sapiens
Source name:
Pancreatic cancer cell line
Platform:
GPL18573
Series:
GSE112845
Download data: MTX, TSV
Sample
Accession:
GSM3087631
ID:
303087631
7.

CD8+_methanol_SSC

Organism:
Homo sapiens
Source name:
whole blood
Platform:
GPL18573
Series:
GSE112845
Download data: MTX, TSV
Sample
Accession:
GSM3087629
ID:
303087629
8.

DTM-Y_PBMC_methanol-3W+SSC

Organism:
Homo sapiens
Source name:
whole blood
Platform:
GPL18573
Series:
GSE112845
Download data: MTX, TSV
Sample
Accession:
GSM3087626
ID:
303087626
9.

DTM-X_PBMC_methanol-3W+SSC

Organism:
Homo sapiens
Source name:
whole blood
Platform:
GPL18573
Series:
GSE112845
Download data: MTX, TSV
Sample
Accession:
GSM3087624
ID:
303087624
10.

DTM-X_PBMC_methanol-3H+SSC

Organism:
Homo sapiens
Source name:
whole blood
Platform:
GPL18573
Series:
GSE112845
Download data: MTX, TSV
Sample
Accession:
GSM3087622
ID:
303087622
11.

DTM-X_PBMC_live

Organism:
Homo sapiens
Source name:
whole blood
Platform:
GPL18573
Series:
GSE112845
Download data: MTX, TSV
Sample
Accession:
GSM3087619
ID:
303087619
12.

CD8+_live

Organism:
Homo sapiens
Source name:
whole blood
Platform:
GPL21290
Series:
GSE112845
Download data: MTX, TSV
Sample
Accession:
GSM3087628
ID:
303087628
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Supplemental Content

db=gds|term=GSE112845[Accession]|query=1|qty=2|blobid=MCID_6748cc53462e1a751e1096c0|ismultiple=true|min_list=5|max_list=20|def_tree=20|def_list=|def_view=|url=/Taxonomy/backend/subset.cgi?|trace_url=/stat?
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