GEO DataSets

(Submitter supplied) Chromosomal double-strand breaks (DSBs) are resected by 5’-nucleases to form 3’ single-strand DNA (ssDNA) substrates for binding by homologous recombination and DNA damage checkpoint proteins. Two redundant pathways of extensive resection were described both in cells and in vitro, one relying on Exo1 exonuclease and the other on Sgs1 helicase and Dna2 nuclease. However, it remains unknown how resection proceeds within the context of chromatin where histones and histone-bound proteins represent barriers for resection enzymes. more...

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae

Type:

Expression profiling by array; Genome variation profiling by array

Platforms:

GPL2529 GPL1444

6 Samples

Download data: CEL, GPR

(Submitter supplied) The goal of this study was to identify yeast mutants with arsenite hypersensitivity. Keywords: dose response

Organism:

Saccharomyces cerevisiae

Type:

Genome variation profiling by array

Platform:

GPL1444

9 Samples

Download data: TIFF

(Submitter supplied) Variants of the essential genes in budding yeast S. cerevisae were cloned into a variomics library, and tested for their ability to confer resistance to three different drugs. Genes were tagged with molecular barcodes, and the relative change in abundance of the molecular barcodes are detected using a spotted Agilent synthesized microarray.

Organism:

Saccharomyces cerevisiae

Type:

Genome variation profiling by array

Platform:

GPL1444

3 Samples

Download data: GPR

(Submitter supplied) Caloric restriction extends lifespan, an effect once thought to involve attenuation of reactive oxygen species (ROS) generated by aerobic metabolism. However, recent evidence suggests that caloric restriction may in fact raise ROS levels, which in turn provides protection from acute doses of oxidant through a process called adaptation. To shed light on the molecular mechanisms of adaptation, we designed a series of genome-wide deletion fitness screens to identify genes involved in adaptation to hydrogen peroxide. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome variation profiling by array

Platform:

GPL1444

10 Samples

Download data: GPR

(Submitter supplied) Histone acetylation and deacetylation are among the principal mechanisms by which chromatin is regulated during transcription, DNA silencing, and DNA repair. We analyzed patterns of genetic interactions uncovered during comprehensive genome-wide analyses in yeast to probe how histone acetyltransferase (HAT) and histone deacetylase (HDAC) protein complexes interact. The genetic interaction data unveil an underappreciated role of HDACs in maintaining cellular viability, and led us to show that deacetylation of the histone variant Htz1p at lysine 14 is mediated by Hda1p. more...

Organism:

Saccharomyces cerevisiae

Type:

Other

Platform:

GPL1444

44 Samples

Download data: GPR, TIFF

(Submitter supplied) Obtain synthetic lethality/slow growth gene interactors of acs2-Ts8 Keywords: gene mutation

Organism:

Saccharomyces cerevisiae

Type:

Other

Platform:

GPL1444

1 Sample

Download data

(Submitter supplied) A network governing DNA integrity was identified in yeast by a global genetic analysis of synthetic fitness or lethality defect (SFL) interactions. Within this network, multiple functional modules or mini-pathways were defined according to their common patterns of global SFL interactions and available protein-protein interaction information. Modules or genes involved in DNA replication, DNA replication checkpoint signaling, and oxidative stress response were identified as the major guardians against lethal spontaneous DNA damage, efficient repair of which requires the functions of the DNA damage checkpoint signaling and multiple DNA repair pathways. more...

Organism:

Saccharomyces cerevisiae

Type:

Other

Platform:

GPL1444

121 Samples

Download data: TIFF

(Submitter supplied) Yeast knockout collection TAG microarrays are an emergent platform for rapid, genome-wide functional characterization of yeast genes. We describe a method for analyzing two-color array data to efficiently represent differential knockout strain representation across two experimental conditions. Using a fully defined spike-in pool, we show that the sensitivity and specificity of this method exceed typical current approaches. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome variation profiling by array

Platform:

GPL1444

5 Samples

Download data

(Submitter supplied) These datasets use diploid-based synthetic lethality analysis on microarrays (dSLAM) as a new technology for identifying genetic interactions of various types. These interactions include synthetic lethality (CIN8, BIM1, SGS1), synthetic haploinsufficiency (TUB1), dosage suppression (LCD1), suppression of a lethal mutation (SML1), suppression of an overexpressed conditionally lethal allele (CDC102), and gene-chemical interactions (Benomyl). more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platforms:

GPL1446 GPL1444 GPL1445

33 Samples

Download data: TIFF

(Submitter supplied) Assessment of synthetic lethality between an sml1 null mutation and Yeast Deletion mutations Keywords = SML1 Keywords = yeast Keywords = deletion Keywords = synthetic lethality Keywords = tag Keywords = barcode Keywords: repeat sample

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL1444

1 Sample

Download data: TIFF

(Submitter supplied) Assessment of growth impairment resulting from a heterozygous tub1 null mutation as compared to that from a heterozygous ura3 null mutation Keywords = TUB1 Keywords = URA3 Keywords = yeast Keywords = deletion Keywords = synthetic haploinsufficiency Keywords = tag Keywords = barcode Keywords: repeat sample

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL1444

2 Samples

Download data: TIFF

(Submitter supplied) Assessment of synthetic lethality between an overexpressed LCD1 gene and Yeast Deletion mutations Keywords = LCD1 Keywords = overexpression Keywords = yeast Keywords = deletion Keywords = synthetic lethality Keywords = tag Keywords = barcode Keywords: repeat sample

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL1444

2 Samples

Download data: TIFF

(Submitter supplied) Assessment of synthetic lethality between a temperature-sensitive allele of CDC102 and Yeast Deletion mutations Keywords: repeat sample

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL1444

4 Samples

Download data: TIFF

(Submitter supplied) Assessment of synthetic lethality between an sgs1 null mutation and Yeast Deletion mutations Keywords = SGS1 Keywords = yeast Keywords = deletion Keywords = synthetic lethality Keywords = tag Keywords = barcode Keywords: repeat sample

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platforms:

GPL1446 GPL1444 GPL1445

5 Samples

Download data: TIFF

(Submitter supplied) Assessment of synthetic lethality between a cin8 deletion mutation and Yeast Deletion mutations. Keywords = CIN8 Keywords = yeast Keywords = deletion Keywords = synthetic lethality Keywords = tag Keywords = barcode Keywords: repeat sample

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL1444

3 Samples

Download data: TIFF

(Submitter supplied) Synthetic lethality of Yeast Deletion strain mutations in haploid cells with various concentrations of Benomyl Keywords = Benomyl Keywords = yeast Keywords = deletion Keywords = tag Keywords = barcode Keywords: dose response

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL1444

10 Samples

Download data: TIFF