Restriction endonuclease EcoO109I and related endonucleases
EcoO109I is a type II restriction endonuclease that recognizes ds DNA with a seven-base pair motif of both degenerate and discontinuous sequence, RG|GNCCY (R = A or G, Y = T or C, with | designating the cleavage site), and generates 5'-overhangs; it belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.
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